Arboreal ant associated circular virus 1
Average proteome isoelectric point is 8.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BP90|A0A346BP90_9CIRC ATP-dependent helicase Rep OS=Arboreal ant associated circular virus 1 OX=2293273 PE=3 SV=1
MM1 pKa = 7.25 NATVRR6 pKa = 11.84 RR7 pKa = 11.84 FVFTLNNYY15 pKa = 9.12 SEE17 pKa = 4.41 EE18 pKa = 4.19 DD19 pKa = 3.36 VTKK22 pKa = 10.58 IKK24 pKa = 11.0 LFINTYY30 pKa = 9.02 CKK32 pKa = 10.57 YY33 pKa = 10.74 GIFGKK38 pKa = 10.35 EE39 pKa = 3.71 LAPTTNTPYY48 pKa = 10.98 LQGFCNLEE56 pKa = 3.5 KK57 pKa = 10.54 PMRR60 pKa = 11.84 FNTVKK65 pKa = 10.5 KK66 pKa = 9.82 HH67 pKa = 5.79 FDD69 pKa = 3.4 NAVHH73 pKa = 6.48 IEE75 pKa = 3.98 KK76 pKa = 10.96 AMGTDD81 pKa = 3.44 EE82 pKa = 5.01 QNQKK86 pKa = 10.19 YY87 pKa = 9.47 CSKK90 pKa = 10.87 SGDD93 pKa = 3.37 IFEE96 pKa = 5.23 KK97 pKa = 9.83 GTPVRR102 pKa = 11.84 QGQRR106 pKa = 11.84 SDD108 pKa = 3.84 LEE110 pKa = 4.54 SMVAAIKK117 pKa = 10.79 DD118 pKa = 3.7 GEE120 pKa = 4.34 TDD122 pKa = 3.27 EE123 pKa = 4.51 KK124 pKa = 10.64 TLAEE128 pKa = 4.96 LNPSCYY134 pKa = 9.32 IRR136 pKa = 11.84 YY137 pKa = 8.87 FRR139 pKa = 11.84 GIRR142 pKa = 11.84 EE143 pKa = 5.06 FIRR146 pKa = 11.84 TIHH149 pKa = 6.85 PIAPRR154 pKa = 11.84 DD155 pKa = 3.62 FKK157 pKa = 11.27 TEE159 pKa = 3.52 VFYY162 pKa = 11.48 YY163 pKa = 9.55 WGPPGTGKK171 pKa = 10.0 SRR173 pKa = 11.84 RR174 pKa = 11.84 ALEE177 pKa = 3.89 EE178 pKa = 4.47 ANTHH182 pKa = 6.76 DD183 pKa = 4.41 SLSVYY188 pKa = 9.63 YY189 pKa = 10.26 KK190 pKa = 10.61 PRR192 pKa = 11.84 GLWWDD197 pKa = 3.99 GYY199 pKa = 10.09 KK200 pKa = 9.59 QQKK203 pKa = 9.44 CVIIDD208 pKa = 4.22 DD209 pKa = 4.5 FYY211 pKa = 11.76 GWIKK215 pKa = 10.75 YY216 pKa = 10.28 DD217 pKa = 4.1 EE218 pKa = 4.44 LLKK221 pKa = 10.71 ICDD224 pKa = 3.53 RR225 pKa = 11.84 YY226 pKa = 9.77 PYY228 pKa = 9.88 KK229 pKa = 11.0 VQVKK233 pKa = 10.42 GGFEE237 pKa = 3.7 EE238 pKa = 5.27 FKK240 pKa = 11.08 AEE242 pKa = 4.54 KK243 pKa = 9.83 IWITSNVDD251 pKa = 2.54 IDD253 pKa = 3.98 EE254 pKa = 4.62 LYY256 pKa = 11.05 KK257 pKa = 10.96 FIGYY261 pKa = 9.56 KK262 pKa = 9.87 PDD264 pKa = 3.3 ALLRR268 pKa = 11.84 RR269 pKa = 11.84 ITNKK273 pKa = 9.85 VYY275 pKa = 10.3 IAA277 pKa = 4.18
Molecular weight: 32.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.803
IPC2_protein 8.009
IPC_protein 7.907
Toseland 7.922
ProMoST 8.404
Dawson 8.668
Bjellqvist 8.799
Wikipedia 8.682
Rodwell 8.726
Grimsley 7.849
Solomon 8.785
Lehninger 8.756
Nozaki 8.887
DTASelect 8.609
Thurlkill 8.682
EMBOSS 8.843
Sillero 8.931
Patrickios 4.202
IPC_peptide 8.77
IPC2_peptide 7.571
IPC2.peptide.svr19 7.784
Protein with the highest isoelectric point:
>tr|A0A346BP90|A0A346BP90_9CIRC ATP-dependent helicase Rep OS=Arboreal ant associated circular virus 1 OX=2293273 PE=3 SV=1
MM1 pKa = 6.84 AWYY4 pKa = 9.94 RR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 APMRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 FRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 8.82 RR20 pKa = 11.84 IIRR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 FVRR29 pKa = 11.84 RR30 pKa = 11.84 NNKK33 pKa = 9.56 GSMFCKK39 pKa = 8.57 FTKK42 pKa = 10.4 VSTVDD47 pKa = 3.23 VPNNQTSIWNASFLPTDD64 pKa = 3.73 FDD66 pKa = 4.28 EE67 pKa = 4.86 FTKK70 pKa = 10.7 LAPNFEE76 pKa = 4.48 YY77 pKa = 11.2 VQFLKK82 pKa = 11.09 VKK84 pKa = 10.08 VRR86 pKa = 11.84 VLPMQSISNNSTSKK100 pKa = 10.4 IPSYY104 pKa = 11.48 CMLPWHH110 pKa = 7.22 RR111 pKa = 11.84 DD112 pKa = 3.51 DD113 pKa = 5.76 PPTKK117 pKa = 9.39 TLSFNSFISQDD128 pKa = 2.69 RR129 pKa = 11.84 CKK131 pKa = 10.18 IYY133 pKa = 10.62 RR134 pKa = 11.84 QTEE137 pKa = 3.96 RR138 pKa = 11.84 GKK140 pKa = 10.85 QMYY143 pKa = 9.49 VPNTLIEE150 pKa = 4.29 ARR152 pKa = 11.84 ASTATDD158 pKa = 3.19 SGAHH162 pKa = 4.08 TVYY165 pKa = 9.99 WKK167 pKa = 10.35 PRR169 pKa = 11.84 VEE171 pKa = 4.13 RR172 pKa = 11.84 TTQTQEE178 pKa = 3.65 LPRR181 pKa = 11.84 IFCGLIAFQGDD192 pKa = 3.28 AALTLQNGTFDD203 pKa = 4.39 IITDD207 pKa = 3.55 VWVKK211 pKa = 10.36 CANQNTLIPP220 pKa = 4.27
Molecular weight: 25.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.78
IPC_protein 10.716
Toseland 10.877
ProMoST 10.657
Dawson 10.95
Bjellqvist 10.701
Wikipedia 11.199
Rodwell 11.125
Grimsley 10.994
Solomon 11.125
Lehninger 11.082
Nozaki 10.877
DTASelect 10.701
Thurlkill 10.877
EMBOSS 11.301
Sillero 10.891
Patrickios 10.833
IPC_peptide 11.125
IPC2_peptide 9.867
IPC2.peptide.svr19 8.548
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
497
220
277
248.5
29.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.03 ± 0.28
2.213 ± 0.039
5.433 ± 0.585
5.231 ± 1.652
6.036 ± 0.216
4.829 ± 1.386
1.207 ± 0.197
6.841 ± 0.615
8.048 ± 1.411
5.634 ± 0.118
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.012 ± 0.472
5.231 ± 0.447
5.03 ± 0.58
3.823 ± 0.777
8.451 ± 2.222
4.829 ± 1.012
7.646 ± 0.953
5.433 ± 0.314
2.012 ± 0.172
5.03 ± 1.519
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here