Amino acid dipepetide frequency for Mycobacterium phage Zavala

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.909AlaAla: 20.909 ± 1.786
1.117AlaCys: 1.117 ± 0.195
7.714AlaAsp: 7.714 ± 0.631
9.204AlaGlu: 9.204 ± 0.876
3.245AlaPhe: 3.245 ± 0.658
9.31AlaGly: 9.31 ± 0.943
3.033AlaHis: 3.033 ± 0.398
5.959AlaIle: 5.959 ± 0.483
4.416AlaLys: 4.416 ± 0.58
11.119AlaLeu: 11.119 ± 0.835
3.458AlaMet: 3.458 ± 0.437
3.352AlaAsn: 3.352 ± 0.414
7.236AlaPro: 7.236 ± 0.776
5.161AlaGln: 5.161 ± 0.579
8.832AlaArg: 8.832 ± 0.631
4.788AlaSer: 4.788 ± 0.539
7.555AlaThr: 7.555 ± 0.781
9.151AlaVal: 9.151 ± 0.687
2.022AlaTrp: 2.022 ± 0.317
2.288AlaTyr: 2.288 ± 0.319
0.0AlaXaa: 0.0 ± 0.0
Cys
0.904CysAla: 0.904 ± 0.242
0.16CysCys: 0.16 ± 0.088
1.011CysAsp: 1.011 ± 0.347
0.851CysGlu: 0.851 ± 0.249
0.213CysPhe: 0.213 ± 0.103
1.49CysGly: 1.49 ± 0.336
0.16CysHis: 0.16 ± 0.086
0.319CysIle: 0.319 ± 0.136
0.16CysLys: 0.16 ± 0.085
0.745CysLeu: 0.745 ± 0.19
0.266CysMet: 0.266 ± 0.136
0.266CysAsn: 0.266 ± 0.108
0.851CysPro: 0.851 ± 0.221
0.266CysGln: 0.266 ± 0.125
0.692CysArg: 0.692 ± 0.186
0.692CysSer: 0.692 ± 0.209
0.798CysThr: 0.798 ± 0.196
0.745CysVal: 0.745 ± 0.205
0.426CysTrp: 0.426 ± 0.132
0.213CysTyr: 0.213 ± 0.105
0.0CysXaa: 0.0 ± 0.0
Asp
7.768AspAla: 7.768 ± 0.733
0.692AspCys: 0.692 ± 0.281
5.799AspAsp: 5.799 ± 0.718
5.693AspGlu: 5.693 ± 0.606
1.596AspPhe: 1.596 ± 0.297
6.438AspGly: 6.438 ± 0.567
0.904AspHis: 0.904 ± 0.222
1.649AspIle: 1.649 ± 0.337
2.022AspLys: 2.022 ± 0.294
5.959AspLeu: 5.959 ± 0.584
1.809AspMet: 1.809 ± 0.321
2.022AspAsn: 2.022 ± 0.316
4.15AspPro: 4.15 ± 0.51
2.235AspGln: 2.235 ± 0.435
3.724AspArg: 3.724 ± 0.503
2.66AspSer: 2.66 ± 0.295
3.245AspThr: 3.245 ± 0.332
4.735AspVal: 4.735 ± 0.472
1.383AspTrp: 1.383 ± 0.294
1.596AspTyr: 1.596 ± 0.326
0.0AspXaa: 0.0 ± 0.0
Glu
8.3GluAla: 8.3 ± 0.743
1.117GluCys: 1.117 ± 0.283
2.447GluAsp: 2.447 ± 0.366
1.596GluGlu: 1.596 ± 0.309
2.128GluPhe: 2.128 ± 0.355
4.203GluGly: 4.203 ± 0.484
1.596GluHis: 1.596 ± 0.308
2.341GluIle: 2.341 ± 0.292
1.49GluLys: 1.49 ± 0.349
6.81GluLeu: 6.81 ± 0.614
1.436GluMet: 1.436 ± 0.226
0.958GluAsn: 0.958 ± 0.239
2.926GluPro: 2.926 ± 0.394
2.66GluGln: 2.66 ± 0.346
5.054GluArg: 5.054 ± 0.631
2.554GluSer: 2.554 ± 0.371
2.979GluThr: 2.979 ± 0.348
5.267GluVal: 5.267 ± 0.694
0.904GluTrp: 0.904 ± 0.215
1.809GluTyr: 1.809 ± 0.274
0.0GluXaa: 0.0 ± 0.0
Phe
2.341PheAla: 2.341 ± 0.388
0.319PheCys: 0.319 ± 0.124
3.139PheAsp: 3.139 ± 0.406
1.436PheGlu: 1.436 ± 0.283
0.479PhePhe: 0.479 ± 0.19
2.501PheGly: 2.501 ± 0.397
0.213PheHis: 0.213 ± 0.098
1.117PheIle: 1.117 ± 0.237
1.383PheLys: 1.383 ± 0.283
2.128PheLeu: 2.128 ± 0.37
0.692PheMet: 0.692 ± 0.203
1.277PheAsn: 1.277 ± 0.317
0.958PhePro: 0.958 ± 0.207
0.638PheGln: 0.638 ± 0.169
1.649PheArg: 1.649 ± 0.357
0.958PheSer: 0.958 ± 0.179
2.022PheThr: 2.022 ± 0.362
1.702PheVal: 1.702 ± 0.267
0.372PheTrp: 0.372 ± 0.127
0.532PheTyr: 0.532 ± 0.197
0.0PheXaa: 0.0 ± 0.0
Gly
10.853GlyAla: 10.853 ± 0.989
1.17GlyCys: 1.17 ± 0.273
5.214GlyAsp: 5.214 ± 0.51
5.214GlyGlu: 5.214 ± 0.501
1.809GlyPhe: 1.809 ± 0.307
10.587GlyGly: 10.587 ± 1.435
1.756GlyHis: 1.756 ± 0.278
2.979GlyIle: 2.979 ± 0.71
3.671GlyLys: 3.671 ± 0.444
6.491GlyLeu: 6.491 ± 0.757
1.702GlyMet: 1.702 ± 0.31
2.554GlyAsn: 2.554 ± 0.365
4.043GlyPro: 4.043 ± 0.698
2.288GlyGln: 2.288 ± 0.359
6.65GlyArg: 6.65 ± 0.5
5.373GlySer: 5.373 ± 0.516
4.948GlyThr: 4.948 ± 0.511
6.863GlyVal: 6.863 ± 0.567
2.288GlyTrp: 2.288 ± 0.264
2.394GlyTyr: 2.394 ± 0.496
0.0GlyXaa: 0.0 ± 0.0
His
2.075HisAla: 2.075 ± 0.319
0.266HisCys: 0.266 ± 0.117
1.383HisAsp: 1.383 ± 0.272
1.436HisGlu: 1.436 ± 0.32
0.319HisPhe: 0.319 ± 0.142
2.447HisGly: 2.447 ± 0.388
0.585HisHis: 0.585 ± 0.188
0.692HisIle: 0.692 ± 0.192
0.319HisLys: 0.319 ± 0.125
2.128HisLeu: 2.128 ± 0.347
0.213HisMet: 0.213 ± 0.12
0.532HisAsn: 0.532 ± 0.137
1.33HisPro: 1.33 ± 0.269
0.585HisGln: 0.585 ± 0.15
1.49HisArg: 1.49 ± 0.305
0.638HisSer: 0.638 ± 0.173
1.649HisThr: 1.649 ± 0.242
2.022HisVal: 2.022 ± 0.354
0.585HisTrp: 0.585 ± 0.205
0.745HisTyr: 0.745 ± 0.217
0.0HisXaa: 0.0 ± 0.0
Ile
5.799IleAla: 5.799 ± 0.555
0.16IleCys: 0.16 ± 0.097
3.299IleAsp: 3.299 ± 0.439
2.82IleGlu: 2.82 ± 0.376
0.851IlePhe: 0.851 ± 0.225
3.884IleGly: 3.884 ± 0.576
0.532IleHis: 0.532 ± 0.182
0.585IleIle: 0.585 ± 0.18
1.49IleLys: 1.49 ± 0.255
2.713IleLeu: 2.713 ± 0.34
0.266IleMet: 0.266 ± 0.124
1.383IleAsn: 1.383 ± 0.247
2.022IlePro: 2.022 ± 0.417
0.851IleGln: 0.851 ± 0.203
2.394IleArg: 2.394 ± 0.363
1.862IleSer: 1.862 ± 0.304
2.181IleThr: 2.181 ± 0.299
3.511IleVal: 3.511 ± 0.467
0.532IleTrp: 0.532 ± 0.184
0.479IleTyr: 0.479 ± 0.159
0.0IleXaa: 0.0 ± 0.0
Lys
3.937LysAla: 3.937 ± 0.531
0.426LysCys: 0.426 ± 0.157
1.277LysAsp: 1.277 ± 0.215
0.904LysGlu: 0.904 ± 0.189
1.383LysPhe: 1.383 ± 0.281
3.245LysGly: 3.245 ± 0.437
0.638LysHis: 0.638 ± 0.188
1.49LysIle: 1.49 ± 0.28
0.798LysLys: 0.798 ± 0.244
3.777LysLeu: 3.777 ± 0.429
0.638LysMet: 0.638 ± 0.188
0.532LysAsn: 0.532 ± 0.177
2.873LysPro: 2.873 ± 0.363
0.745LysGln: 0.745 ± 0.209
3.192LysArg: 3.192 ± 0.461
1.383LysSer: 1.383 ± 0.256
2.075LysThr: 2.075 ± 0.297
3.352LysVal: 3.352 ± 0.494
0.585LysTrp: 0.585 ± 0.166
0.638LysTyr: 0.638 ± 0.224
0.0LysXaa: 0.0 ± 0.0
Leu
12.29LeuAla: 12.29 ± 0.951
0.692LeuCys: 0.692 ± 0.218
7.608LeuAsp: 7.608 ± 0.581
2.767LeuGlu: 2.767 ± 0.432
2.075LeuPhe: 2.075 ± 0.349
7.236LeuGly: 7.236 ± 0.663
2.075LeuHis: 2.075 ± 0.359
3.511LeuIle: 3.511 ± 0.415
2.501LeuLys: 2.501 ± 0.39
5.373LeuLeu: 5.373 ± 0.521
1.809LeuMet: 1.809 ± 0.299
2.181LeuAsn: 2.181 ± 0.33
4.15LeuPro: 4.15 ± 0.477
2.82LeuGln: 2.82 ± 0.524
6.65LeuArg: 6.65 ± 0.792
5.267LeuSer: 5.267 ± 0.359
4.575LeuThr: 4.575 ± 0.572
6.384LeuVal: 6.384 ± 0.523
1.702LeuTrp: 1.702 ± 0.323
1.702LeuTyr: 1.702 ± 0.338
0.0LeuXaa: 0.0 ± 0.0
Met
2.607MetAla: 2.607 ± 0.357
0.106MetCys: 0.106 ± 0.081
0.745MetAsp: 0.745 ± 0.183
0.745MetGlu: 0.745 ± 0.196
0.745MetPhe: 0.745 ± 0.205
1.49MetGly: 1.49 ± 0.264
0.585MetHis: 0.585 ± 0.181
1.17MetIle: 1.17 ± 0.241
0.692MetLys: 0.692 ± 0.218
1.969MetLeu: 1.969 ± 0.333
0.372MetMet: 0.372 ± 0.178
0.585MetAsn: 0.585 ± 0.17
1.277MetPro: 1.277 ± 0.274
0.904MetGln: 0.904 ± 0.202
1.064MetArg: 1.064 ± 0.289
2.873MetSer: 2.873 ± 0.292
1.809MetThr: 1.809 ± 0.342
1.277MetVal: 1.277 ± 0.243
0.372MetTrp: 0.372 ± 0.133
0.532MetTyr: 0.532 ± 0.188
0.0MetXaa: 0.0 ± 0.0
Asn
3.618AsnAla: 3.618 ± 0.555
0.319AsnCys: 0.319 ± 0.131
1.33AsnAsp: 1.33 ± 0.274
1.649AsnGlu: 1.649 ± 0.318
0.585AsnPhe: 0.585 ± 0.156
3.245AsnGly: 3.245 ± 0.482
0.426AsnHis: 0.426 ± 0.157
0.958AsnIle: 0.958 ± 0.24
0.851AsnLys: 0.851 ± 0.203
2.022AsnLeu: 2.022 ± 0.337
0.585AsnMet: 0.585 ± 0.154
0.426AsnAsn: 0.426 ± 0.16
2.66AsnPro: 2.66 ± 0.318
0.638AsnGln: 0.638 ± 0.17
1.49AsnArg: 1.49 ± 0.301
1.117AsnSer: 1.117 ± 0.284
1.596AsnThr: 1.596 ± 0.275
2.181AsnVal: 2.181 ± 0.278
0.479AsnTrp: 0.479 ± 0.162
0.638AsnTyr: 0.638 ± 0.183
0.0AsnXaa: 0.0 ± 0.0
Pro
7.98ProAla: 7.98 ± 0.623
0.426ProCys: 0.426 ± 0.168
3.618ProAsp: 3.618 ± 0.497
4.15ProGlu: 4.15 ± 0.524
1.33ProPhe: 1.33 ± 0.244
5.959ProGly: 5.959 ± 0.597
1.011ProHis: 1.011 ± 0.275
1.756ProIle: 1.756 ± 0.205
2.447ProLys: 2.447 ± 0.365
3.937ProLeu: 3.937 ± 0.563
1.436ProMet: 1.436 ± 0.292
1.33ProAsn: 1.33 ± 0.364
2.873ProPro: 2.873 ± 0.392
1.543ProGln: 1.543 ± 0.304
3.405ProArg: 3.405 ± 0.524
3.511ProSer: 3.511 ± 0.555
3.352ProThr: 3.352 ± 0.454
4.363ProVal: 4.363 ± 0.447
1.224ProTrp: 1.224 ± 0.251
1.383ProTyr: 1.383 ± 0.289
0.0ProXaa: 0.0 ± 0.0
Gln
5.48GlnAla: 5.48 ± 0.642
0.372GlnCys: 0.372 ± 0.154
1.011GlnAsp: 1.011 ± 0.17
1.649GlnGlu: 1.649 ± 0.269
0.798GlnPhe: 0.798 ± 0.203
1.969GlnGly: 1.969 ± 0.3
1.17GlnHis: 1.17 ± 0.223
1.862GlnIle: 1.862 ± 0.227
1.17GlnLys: 1.17 ± 0.244
3.033GlnLeu: 3.033 ± 0.419
1.277GlnMet: 1.277 ± 0.253
0.638GlnAsn: 0.638 ± 0.209
2.022GlnPro: 2.022 ± 0.343
1.383GlnGln: 1.383 ± 0.238
2.713GlnArg: 2.713 ± 0.39
1.011GlnSer: 1.011 ± 0.294
1.702GlnThr: 1.702 ± 0.277
3.033GlnVal: 3.033 ± 0.414
0.958GlnTrp: 0.958 ± 0.234
0.958GlnTyr: 0.958 ± 0.203
0.0GlnXaa: 0.0 ± 0.0
Arg
6.916ArgAla: 6.916 ± 0.694
1.011ArgCys: 1.011 ± 0.262
4.469ArgAsp: 4.469 ± 0.651
4.522ArgGlu: 4.522 ± 0.543
1.862ArgPhe: 1.862 ± 0.376
5.054ArgGly: 5.054 ± 0.572
1.596ArgHis: 1.596 ± 0.412
2.979ArgIle: 2.979 ± 0.477
3.299ArgLys: 3.299 ± 0.452
6.491ArgLeu: 6.491 ± 0.578
1.809ArgMet: 1.809 ± 0.314
2.501ArgAsn: 2.501 ± 0.427
3.192ArgPro: 3.192 ± 0.405
3.831ArgGln: 3.831 ± 0.539
6.65ArgArg: 6.65 ± 0.834
3.352ArgSer: 3.352 ± 0.434
3.884ArgThr: 3.884 ± 0.583
4.309ArgVal: 4.309 ± 0.483
2.022ArgTrp: 2.022 ± 0.304
2.075ArgTyr: 2.075 ± 0.387
0.0ArgXaa: 0.0 ± 0.0
Ser
6.916SerAla: 6.916 ± 0.736
0.479SerCys: 0.479 ± 0.203
2.926SerAsp: 2.926 ± 0.338
3.086SerGlu: 3.086 ± 0.378
1.809SerPhe: 1.809 ± 0.333
4.203SerGly: 4.203 ± 0.497
0.851SerHis: 0.851 ± 0.288
1.915SerIle: 1.915 ± 0.388
1.277SerLys: 1.277 ± 0.242
4.15SerLeu: 4.15 ± 0.397
1.33SerMet: 1.33 ± 0.242
1.649SerAsn: 1.649 ± 0.258
3.618SerPro: 3.618 ± 0.437
1.543SerGln: 1.543 ± 0.242
3.033SerArg: 3.033 ± 0.445
2.767SerSer: 2.767 ± 0.477
2.82SerThr: 2.82 ± 0.478
4.15SerVal: 4.15 ± 0.486
1.277SerTrp: 1.277 ± 0.266
1.064SerTyr: 1.064 ± 0.248
0.0SerXaa: 0.0 ± 0.0
Thr
6.916ThrAla: 6.916 ± 0.99
0.692ThrCys: 0.692 ± 0.195
3.884ThrAsp: 3.884 ± 0.515
3.458ThrGlu: 3.458 ± 0.36
1.809ThrPhe: 1.809 ± 0.267
5.161ThrGly: 5.161 ± 0.631
1.277ThrHis: 1.277 ± 0.296
2.66ThrIle: 2.66 ± 0.439
2.288ThrLys: 2.288 ± 0.322
4.097ThrLeu: 4.097 ± 0.491
0.532ThrMet: 0.532 ± 0.162
1.277ThrAsn: 1.277 ± 0.348
3.777ThrPro: 3.777 ± 0.374
1.702ThrGln: 1.702 ± 0.321
3.618ThrArg: 3.618 ± 0.51
3.458ThrSer: 3.458 ± 0.574
3.086ThrThr: 3.086 ± 0.404
5.373ThrVal: 5.373 ± 0.495
1.17ThrTrp: 1.17 ± 0.225
1.436ThrTyr: 1.436 ± 0.304
0.0ThrXaa: 0.0 ± 0.0
Val
9.683ValAla: 9.683 ± 0.775
1.17ValCys: 1.17 ± 0.278
6.225ValAsp: 6.225 ± 0.586
5.746ValGlu: 5.746 ± 0.691
1.543ValPhe: 1.543 ± 0.315
6.544ValGly: 6.544 ± 0.694
2.288ValHis: 2.288 ± 0.499
2.501ValIle: 2.501 ± 0.368
2.607ValLys: 2.607 ± 0.413
5.48ValLeu: 5.48 ± 0.619
1.49ValMet: 1.49 ± 0.272
2.022ValAsn: 2.022 ± 0.347
5.48ValPro: 5.48 ± 0.6
2.075ValGln: 2.075 ± 0.339
5.267ValArg: 5.267 ± 0.57
4.043ValSer: 4.043 ± 0.413
4.416ValThr: 4.416 ± 0.481
6.065ValVal: 6.065 ± 0.648
2.447ValTrp: 2.447 ± 0.381
1.383ValTyr: 1.383 ± 0.329
0.0ValXaa: 0.0 ± 0.0
Trp
2.181TrpAla: 2.181 ± 0.352
0.319TrpCys: 0.319 ± 0.126
1.064TrpAsp: 1.064 ± 0.293
0.638TrpGlu: 0.638 ± 0.168
0.479TrpPhe: 0.479 ± 0.155
1.17TrpGly: 1.17 ± 0.209
0.532TrpHis: 0.532 ± 0.166
0.745TrpIle: 0.745 ± 0.215
0.372TrpLys: 0.372 ± 0.122
2.979TrpLeu: 2.979 ± 0.413
0.479TrpMet: 0.479 ± 0.149
0.532TrpAsn: 0.532 ± 0.146
1.011TrpPro: 1.011 ± 0.228
1.117TrpGln: 1.117 ± 0.201
2.713TrpArg: 2.713 ± 0.389
1.277TrpSer: 1.277 ± 0.248
1.383TrpThr: 1.383 ± 0.239
1.756TrpVal: 1.756 ± 0.308
0.426TrpTrp: 0.426 ± 0.144
0.426TrpTyr: 0.426 ± 0.11
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.394TyrAla: 2.394 ± 0.287
0.213TyrCys: 0.213 ± 0.099
1.915TyrAsp: 1.915 ± 0.365
1.011TyrGlu: 1.011 ± 0.24
0.798TyrPhe: 0.798 ± 0.207
2.713TyrGly: 2.713 ± 0.435
0.213TyrHis: 0.213 ± 0.106
0.426TyrIle: 0.426 ± 0.148
0.638TyrLys: 0.638 ± 0.201
1.969TyrLeu: 1.969 ± 0.373
0.266TyrMet: 0.266 ± 0.107
0.798TyrAsn: 0.798 ± 0.169
0.638TyrPro: 0.638 ± 0.156
1.224TyrGln: 1.224 ± 0.24
1.543TyrArg: 1.543 ± 0.278
1.277TyrSer: 1.277 ± 0.269
1.596TyrThr: 1.596 ± 0.255
2.235TyrVal: 2.235 ± 0.386
0.426TyrTrp: 0.426 ± 0.196
0.532TyrTyr: 0.532 ± 0.164
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 97 proteins (18797 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski