Desulfosporosinus sp. OT

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfosporosinus; unclassified Desulfosporosinus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6205 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2FQI4|G2FQI4_9FIRM Stage 0 sporulation protein A homolog OS=Desulfosporosinus sp. OT OX=913865 GN=DOT_1991 PE=4 SV=1
MM1 pKa = 7.6RR2 pKa = 11.84VSKK5 pKa = 10.74LLATIVMTLLISTNINVSTVLAATVIDD32 pKa = 4.56GGPATVFVHH41 pKa = 6.68SSTSEE46 pKa = 3.87VEE48 pKa = 3.97SANRR52 pKa = 11.84IVADD56 pKa = 3.65TANPGLGKK64 pKa = 8.7ITSITLKK71 pKa = 10.84GYY73 pKa = 9.78AAQSNLKK80 pKa = 10.2VMYY83 pKa = 9.72DD84 pKa = 3.15SWAEE88 pKa = 3.77PVMWYY93 pKa = 9.97YY94 pKa = 11.55AEE96 pKa = 4.61TIINTTITPSGSPSLAYY113 pKa = 10.51LKK115 pKa = 10.04IYY117 pKa = 10.18PNGEE121 pKa = 3.73DD122 pKa = 2.94NYY124 pKa = 10.08IYY126 pKa = 10.79VSSFSATNAVGDD138 pKa = 3.86HH139 pKa = 5.73FVVNFTSPTNYY150 pKa = 9.68VPPDD154 pKa = 4.42PDD156 pKa = 4.95PDD158 pKa = 4.82PDD160 pKa = 5.08PDD162 pKa = 5.1PDD164 pKa = 4.93PDD166 pKa = 4.72PNPDD170 pKa = 4.46PDD172 pKa = 5.11PDD174 pKa = 4.72PDD176 pKa = 4.6PEE178 pKa = 5.67PEE180 pKa = 4.19LCEE183 pKa = 4.14STLL186 pKa = 3.61

Molecular weight: 19.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2FU94|G2FU94_9FIRM 6-carboxyhexanoate--CoA ligase OS=Desulfosporosinus sp. OT OX=913865 GN=bioW PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 9.19QPKK8 pKa = 8.78NRR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 5.44KK13 pKa = 10.09RR14 pKa = 11.84VHH16 pKa = 5.93GFLSRR21 pKa = 11.84MSTPTGRR28 pKa = 11.84NVIKK32 pKa = 10.5RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.52GRR39 pKa = 11.84KK40 pKa = 8.8KK41 pKa = 10.81LSVV44 pKa = 3.15

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6205

0

6205

1563146

37

2894

251.9

28.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.467 ± 0.037

1.151 ± 0.018

4.942 ± 0.027

6.691 ± 0.039

4.137 ± 0.025

7.222 ± 0.033

1.86 ± 0.014

7.568 ± 0.031

6.217 ± 0.028

10.397 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.674 ± 0.015

4.274 ± 0.024

3.84 ± 0.021

3.805 ± 0.024

4.617 ± 0.024

6.214 ± 0.027

5.446 ± 0.026

7.151 ± 0.029

1.072 ± 0.012

3.255 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski