Corynebacterium marinum DSM 44953
Average proteome isoelectric point is 5.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2547 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B6TQJ8|A0A0B6TQJ8_9CORY Uncharacterized protein OS=Corynebacterium marinum DSM 44953 OX=1224162 GN=B840_04600 PE=4 SV=1
MM1 pKa = 7.42 KK2 pKa = 9.59 RR3 pKa = 11.84 TIALAALALTSSLALAACTDD23 pKa = 4.12 DD24 pKa = 3.91 TASDD28 pKa = 3.8 APEE31 pKa = 4.16 TTTTTTSEE39 pKa = 4.13 TATQSSEE46 pKa = 3.85 EE47 pKa = 4.1 TGMNDD52 pKa = 2.87 QMGAEE57 pKa = 4.39 GHH59 pKa = 6.37 DD60 pKa = 3.65 HH61 pKa = 6.84 PADD64 pKa = 4.23 GGQPPAGIAAEE75 pKa = 4.23 EE76 pKa = 4.32 NPTYY80 pKa = 10.41 PVDD83 pKa = 3.76 TEE85 pKa = 4.44 VVLTADD91 pKa = 4.25 HH92 pKa = 6.13 MPGMEE97 pKa = 4.12 GAEE100 pKa = 3.97 ATIAGAFDD108 pKa = 3.33 TTTYY112 pKa = 10.32 SVSYY116 pKa = 9.57 TPTDD120 pKa = 3.32 GGDD123 pKa = 3.15 PVTDD127 pKa = 3.61 HH128 pKa = 6.6 RR129 pKa = 11.84 WVVHH133 pKa = 6.18 EE134 pKa = 4.48 EE135 pKa = 3.95 LVDD138 pKa = 4.02 PGQAPLPEE146 pKa = 5.0 GSEE149 pKa = 4.15 VVLDD153 pKa = 3.97 AEE155 pKa = 5.02 HH156 pKa = 6.2 MSGMKK161 pKa = 10.16 GVEE164 pKa = 3.67 ATIDD168 pKa = 3.56 YY169 pKa = 8.7 STDD172 pKa = 2.91 EE173 pKa = 4.18 TVYY176 pKa = 10.42 MVDD179 pKa = 3.47 LTVDD183 pKa = 3.48 GMTMTNHH190 pKa = 5.38 KK191 pKa = 9.32 WVTEE195 pKa = 4.08 SEE197 pKa = 4.25 IAPAEE202 pKa = 3.99
Molecular weight: 21.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.808
IPC_protein 3.795
Toseland 3.592
ProMoST 3.923
Dawson 3.77
Bjellqvist 3.948
Wikipedia 3.681
Rodwell 3.617
Grimsley 3.503
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.062
Thurlkill 3.63
EMBOSS 3.694
Sillero 3.91
Patrickios 1.863
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.827
Protein with the highest isoelectric point:
>tr|A0A0B6TT99|A0A0B6TT99_9CORY Cytidylate kinase OS=Corynebacterium marinum DSM 44953 OX=1224162 GN=cmk PE=3 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.63 KK15 pKa = 9.39 KK16 pKa = 9.04 HH17 pKa = 5.39 RR18 pKa = 11.84 KK19 pKa = 5.37 MLRR22 pKa = 11.84 RR23 pKa = 11.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 10.11 LGKK33 pKa = 9.87
Molecular weight: 4.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2547
0
2547
816021
29
3036
320.4
34.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.143 ± 0.069
0.601 ± 0.012
6.149 ± 0.041
6.266 ± 0.05
3.181 ± 0.027
8.926 ± 0.037
2.223 ± 0.028
4.592 ± 0.038
2.402 ± 0.036
10.128 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.157 ± 0.023
2.38 ± 0.03
5.349 ± 0.04
2.96 ± 0.027
6.972 ± 0.057
5.372 ± 0.029
6.067 ± 0.033
8.622 ± 0.046
1.452 ± 0.022
2.058 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here