Amino acid dipepetide frequency for Plasmopara halstedii (Downy mildew of sunflower)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.032AlaAla: 7.032 ± 0.051	1.273AlaCys: 1.273 ± 0.017	3.683AlaAsp: 3.683 ± 0.024	4.45AlaGlu: 4.45 ± 0.028	3.091AlaPhe: 3.091 ± 0.022	3.861AlaGly: 3.861 ± 0.032	1.7AlaHis: 1.7 ± 0.017	4.241AlaIle: 4.241 ± 0.029	4.336AlaLys: 4.336 ± 0.032	8.037AlaLeu: 8.037 ± 0.057	2.061AlaMet: 2.061 ± 0.019	2.999AlaAsn: 2.999 ± 0.025	2.962AlaPro: 2.962 ± 0.029	3.221AlaGln: 3.221 ± 0.027	4.252AlaArg: 4.252 ± 0.034	6.594AlaSer: 6.594 ± 0.041	4.841AlaThr: 4.841 ± 0.037	5.206AlaVal: 5.206 ± 0.035	0.763AlaTrp: 0.763 ± 0.011	1.873AlaTyr: 1.873 ± 0.017	0.005AlaXaa: 0.005 ± 0.001
Cys	1.315CysAla: 1.315 ± 0.018	0.417CysCys: 0.417 ± 0.011	0.989CysAsp: 0.989 ± 0.014	1.02CysGlu: 1.02 ± 0.014	0.773CysPhe: 0.773 ± 0.012	1.221CysGly: 1.221 ± 0.017	0.484CysHis: 0.484 ± 0.01	0.992CysIle: 0.992 ± 0.013	0.868CysLys: 0.868 ± 0.013	1.618CysLeu: 1.618 ± 0.018	0.442CysMet: 0.442 ± 0.008	0.709CysAsn: 0.709 ± 0.011	0.781CysPro: 0.781 ± 0.025	0.679CysGln: 0.679 ± 0.011	1.079CysArg: 1.079 ± 0.017	1.53CysSer: 1.53 ± 0.02	1.055CysThr: 1.055 ± 0.013	1.3CysVal: 1.3 ± 0.015	0.226CysTrp: 0.226 ± 0.006	0.501CysTyr: 0.501 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	4.496AspAla: 4.496 ± 0.028	0.909AspCys: 0.909 ± 0.013	4.108AspAsp: 4.108 ± 0.038	4.608AspGlu: 4.608 ± 0.038	2.3AspPhe: 2.3 ± 0.022	3.375AspGly: 3.375 ± 0.026	1.252AspHis: 1.252 ± 0.015	2.983AspIle: 2.983 ± 0.026	2.822AspLys: 2.822 ± 0.023	5.036AspLeu: 5.036 ± 0.03	1.378AspMet: 1.378 ± 0.014	2.177AspAsn: 2.177 ± 0.021	2.237AspPro: 2.237 ± 0.02	2.018AspGln: 2.018 ± 0.019	2.841AspArg: 2.841 ± 0.023	4.186AspSer: 4.186 ± 0.026	3.017AspThr: 3.017 ± 0.024	4.297AspVal: 4.297 ± 0.032	0.631AspTrp: 0.631 ± 0.01	1.441AspTyr: 1.441 ± 0.014	0.002AspXaa: 0.002 ± 0.001
Glu	4.945GluAla: 4.945 ± 0.031	1.013GluCys: 1.013 ± 0.016	3.786GluAsp: 3.786 ± 0.034	5.035GluGlu: 5.035 ± 0.053	2.308GluPhe: 2.308 ± 0.02	2.732GluGly: 2.732 ± 0.024	1.569GluHis: 1.569 ± 0.019	3.567GluIle: 3.567 ± 0.025	4.238GluLys: 4.238 ± 0.037	6.543GluLeu: 6.543 ± 0.044	1.908GluMet: 1.908 ± 0.02	3.007GluAsn: 3.007 ± 0.027	1.881GluPro: 1.881 ± 0.019	2.83GluGln: 2.83 ± 0.029	3.994GluArg: 3.994 ± 0.032	4.641GluSer: 4.641 ± 0.031	3.562GluThr: 3.562 ± 0.028	4.04GluVal: 4.04 ± 0.03	0.723GluTrp: 0.723 ± 0.012	1.723GluTyr: 1.723 ± 0.016	0.003GluXaa: 0.003 ± 0.001
Phe	3.02PheAla: 3.02 ± 0.026	0.849PheCys: 0.849 ± 0.014	2.505PheAsp: 2.505 ± 0.021	2.536PheGlu: 2.536 ± 0.024	1.71PhePhe: 1.71 ± 0.02	2.623PheGly: 2.623 ± 0.031	1.051PheHis: 1.051 ± 0.014	1.785PheIle: 1.785 ± 0.019	1.77PheLys: 1.77 ± 0.018	3.787PheLeu: 3.787 ± 0.03	0.924PheMet: 0.924 ± 0.013	1.457PheAsn: 1.457 ± 0.016	1.611PhePro: 1.611 ± 0.019	1.729PheGln: 1.729 ± 0.02	2.188PheArg: 2.188 ± 0.02	3.243PheSer: 3.243 ± 0.027	2.141PheThr: 2.141 ± 0.023	3.003PheVal: 3.003 ± 0.026	0.455PheTrp: 0.455 ± 0.008	1.246PheTyr: 1.246 ± 0.014	0.002PheXaa: 0.002 ± 0.001
Gly	3.791GlyAla: 3.791 ± 0.037	1.006GlyCys: 1.006 ± 0.016	3.144GlyAsp: 3.144 ± 0.031	3.007GlyGlu: 3.007 ± 0.028	2.247GlyPhe: 2.247 ± 0.022	3.771GlyGly: 3.771 ± 0.066	1.381GlyHis: 1.381 ± 0.016	3.006GlyIle: 3.006 ± 0.028	3.089GlyLys: 3.089 ± 0.024	4.7GlyLeu: 4.7 ± 0.035	1.376GlyMet: 1.376 ± 0.019	2.427GlyAsn: 2.427 ± 0.028	1.73GlyPro: 1.73 ± 0.019	1.853GlyGln: 1.853 ± 0.022	2.992GlyArg: 2.992 ± 0.025	4.656GlySer: 4.656 ± 0.051	3.055GlyThr: 3.055 ± 0.037	3.852GlyVal: 3.852 ± 0.033	0.63GlyTrp: 0.63 ± 0.012	1.653GlyTyr: 1.653 ± 0.019	0.003GlyXaa: 0.003 ± 0.001
His	1.79HisAla: 1.79 ± 0.017	0.498HisCys: 0.498 ± 0.009	1.432HisAsp: 1.432 ± 0.015	1.678HisGlu: 1.678 ± 0.018	1.171HisPhe: 1.171 ± 0.016	1.509HisGly: 1.509 ± 0.018	0.825HisHis: 0.825 ± 0.013	1.209HisIle: 1.209 ± 0.016	1.153HisLys: 1.153 ± 0.016	2.563HisLeu: 2.563 ± 0.022	0.592HisMet: 0.592 ± 0.011	0.93HisAsn: 0.93 ± 0.013	1.175HisPro: 1.175 ± 0.015	1.168HisGln: 1.168 ± 0.014	1.61HisArg: 1.61 ± 0.017	1.956HisSer: 1.956 ± 0.02	1.272HisThr: 1.272 ± 0.015	1.867HisVal: 1.867 ± 0.019	0.3HisTrp: 0.3 ± 0.007	0.726HisTyr: 0.726 ± 0.011	0.001HisXaa: 0.001 ± 0.0
Ile	4.165IleAla: 4.165 ± 0.028	1.029IleCys: 1.029 ± 0.017	3.158IleAsp: 3.158 ± 0.027	3.447IleGlu: 3.447 ± 0.025	2.037IlePhe: 2.037 ± 0.021	2.785IleGly: 2.785 ± 0.025	1.203IleHis: 1.203 ± 0.017	2.392IleIle: 2.392 ± 0.025	2.785IleLys: 2.785 ± 0.024	4.678IleLeu: 4.678 ± 0.032	1.198IleMet: 1.198 ± 0.014	2.083IleAsn: 2.083 ± 0.021	2.32IlePro: 2.32 ± 0.022	2.189IleGln: 2.189 ± 0.021	2.854IleArg: 2.854 ± 0.02	4.248IleSer: 4.248 ± 0.032	2.76IleThr: 2.76 ± 0.027	3.75IleVal: 3.75 ± 0.028	0.569IleTrp: 0.569 ± 0.011	1.39IleTyr: 1.39 ± 0.016	0.002IleXaa: 0.002 ± 0.001
Lys	4.12LysAla: 4.12 ± 0.033	0.98LysCys: 0.98 ± 0.014	2.812LysAsp: 2.812 ± 0.025	3.729LysGlu: 3.729 ± 0.037	1.993LysPhe: 1.993 ± 0.019	2.385LysGly: 2.385 ± 0.022	1.443LysHis: 1.443 ± 0.018	2.817LysIle: 2.817 ± 0.024	4.239LysLys: 4.239 ± 0.046	5.734LysLeu: 5.734 ± 0.032	1.532LysMet: 1.532 ± 0.017	2.457LysAsn: 2.457 ± 0.023	2.028LysPro: 2.028 ± 0.019	2.617LysGln: 2.617 ± 0.031	4.03LysArg: 4.03 ± 0.036	4.36LysSer: 4.36 ± 0.027	3.303LysThr: 3.303 ± 0.025	3.407LysVal: 3.407 ± 0.025	0.724LysTrp: 0.724 ± 0.012	1.626LysTyr: 1.626 ± 0.016	0.002LysXaa: 0.002 ± 0.0
Leu	7.497LeuAla: 7.497 ± 0.046	1.817LeuCys: 1.817 ± 0.019	5.319LeuAsp: 5.319 ± 0.035	6.446LeuGlu: 6.446 ± 0.046	3.618LeuPhe: 3.618 ± 0.036	4.654LeuGly: 4.654 ± 0.031	2.772LeuHis: 2.772 ± 0.023	4.26LeuIle: 4.26 ± 0.031	5.521LeuLys: 5.521 ± 0.036	10.315LeuLeu: 10.315 ± 0.076	2.318LeuMet: 2.318 ± 0.02	3.986LeuAsn: 3.986 ± 0.027	4.428LeuPro: 4.428 ± 0.034	5.084LeuGln: 5.084 ± 0.035	6.395LeuArg: 6.395 ± 0.044	8.242LeuSer: 8.242 ± 0.041	5.395LeuThr: 5.395 ± 0.034	6.347LeuVal: 6.347 ± 0.041	1.033LeuTrp: 1.033 ± 0.014	2.642LeuTyr: 2.642 ± 0.025	0.003LeuXaa: 0.003 ± 0.001
Met	1.988MetAla: 1.988 ± 0.02	0.414MetCys: 0.414 ± 0.009	1.402MetAsp: 1.402 ± 0.016	1.724MetGlu: 1.724 ± 0.017	0.845MetPhe: 0.845 ± 0.013	1.263MetGly: 1.263 ± 0.015	0.664MetHis: 0.664 ± 0.011	1.189MetIle: 1.189 ± 0.015	1.572MetLys: 1.572 ± 0.019	2.587MetLeu: 2.587 ± 0.022	0.72MetMet: 0.72 ± 0.012	1.097MetAsn: 1.097 ± 0.014	1.065MetPro: 1.065 ± 0.015	1.187MetGln: 1.187 ± 0.016	1.535MetArg: 1.535 ± 0.019	2.041MetSer: 2.041 ± 0.019	1.666MetThr: 1.666 ± 0.016	1.49MetVal: 1.49 ± 0.017	0.301MetTrp: 0.301 ± 0.008	0.681MetTyr: 0.681 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	3.349AsnAla: 3.349 ± 0.026	0.766AsnCys: 0.766 ± 0.012	2.659AsnAsp: 2.659 ± 0.024	3.011AsnGlu: 3.011 ± 0.024	1.697AsnPhe: 1.697 ± 0.02	2.767AsnGly: 2.767 ± 0.026	0.998AsnHis: 0.998 ± 0.013	2.154AsnIle: 2.154 ± 0.019	2.051AsnLys: 2.051 ± 0.019	3.73AsnLeu: 3.73 ± 0.03	1.026AsnMet: 1.026 ± 0.015	1.72AsnAsn: 1.72 ± 0.023	1.746AsnPro: 1.746 ± 0.018	1.624AsnGln: 1.624 ± 0.017	2.164AsnArg: 2.164 ± 0.022	3.377AsnSer: 3.377 ± 0.026	2.263AsnThr: 2.263 ± 0.022	3.143AsnVal: 3.143 ± 0.022	0.453AsnTrp: 0.453 ± 0.008	1.128AsnTyr: 1.128 ± 0.014	0.002AsnXaa: 0.002 ± 0.001
Pro	2.891ProAla: 2.891 ± 0.025	0.594ProCys: 0.594 ± 0.012	2.112ProAsp: 2.112 ± 0.02	2.5ProGlu: 2.5 ± 0.024	1.66ProPhe: 1.66 ± 0.019	2.014ProGly: 2.014 ± 0.024	1.054ProHis: 1.054 ± 0.014	2.123ProIle: 2.123 ± 0.018	2.039ProLys: 2.039 ± 0.021	3.974ProLeu: 3.974 ± 0.029	0.965ProMet: 0.965 ± 0.013	1.673ProAsn: 1.673 ± 0.02	2.51ProPro: 2.51 ± 0.034	1.632ProGln: 1.632 ± 0.018	2.276ProArg: 2.276 ± 0.021	4.078ProSer: 4.078 ± 0.029	2.854ProThr: 2.854 ± 0.029	2.85ProVal: 2.85 ± 0.027	0.434ProTrp: 0.434 ± 0.01	1.049ProTyr: 1.049 ± 0.012	0.002ProXaa: 0.002 ± 0.001
Gln	3.01GlnAla: 3.01 ± 0.028	0.727GlnCys: 0.727 ± 0.012	2.065GlnAsp: 2.065 ± 0.019	2.747GlnGlu: 2.747 ± 0.026	1.531GlnPhe: 1.531 ± 0.016	1.677GlnGly: 1.677 ± 0.016	1.346GlnHis: 1.346 ± 0.015	2.236GlnIle: 2.236 ± 0.019	2.504GlnLys: 2.504 ± 0.023	4.818GlnLeu: 4.818 ± 0.035	1.172GlnMet: 1.172 ± 0.013	1.859GlnAsn: 1.859 ± 0.019	1.586GlnPro: 1.586 ± 0.017	2.815GlnGln: 2.815 ± 0.037	3.096GlnArg: 3.096 ± 0.029	3.335GlnSer: 3.335 ± 0.03	2.41GlnThr: 2.41 ± 0.023	2.705GlnVal: 2.705 ± 0.024	0.537GlnTrp: 0.537 ± 0.01	1.171GlnTyr: 1.171 ± 0.013	0.001GlnXaa: 0.001 ± 0.0
Arg	4.209ArgAla: 4.209 ± 0.029	1.102ArgCys: 1.102 ± 0.015	3.084ArgAsp: 3.084 ± 0.024	3.714ArgGlu: 3.714 ± 0.034	2.439ArgPhe: 2.439 ± 0.022	3.05ArgGly: 3.05 ± 0.024	1.715ArgHis: 1.715 ± 0.018	3.048ArgIle: 3.048 ± 0.025	3.726ArgLys: 3.726 ± 0.035	6.035ArgLeu: 6.035 ± 0.038	1.559ArgMet: 1.559 ± 0.017	2.534ArgAsn: 2.534 ± 0.025	2.159ArgPro: 2.159 ± 0.019	2.891ArgGln: 2.891 ± 0.03	4.698ArgArg: 4.698 ± 0.042	4.859ArgSer: 4.859 ± 0.037	3.007ArgThr: 3.007 ± 0.026	3.878ArgVal: 3.878 ± 0.028	0.745ArgTrp: 0.745 ± 0.012	1.672ArgTyr: 1.672 ± 0.019	0.002ArgXaa: 0.002 ± 0.001
Ser	6.151SerAla: 6.151 ± 0.04	1.506SerCys: 1.506 ± 0.019	4.699SerAsp: 4.699 ± 0.033	4.56SerGlu: 4.56 ± 0.033	3.386SerPhe: 3.386 ± 0.029	4.793SerGly: 4.793 ± 0.068	1.937SerHis: 1.937 ± 0.019	4.518SerIle: 4.518 ± 0.032	4.574SerLys: 4.574 ± 0.034	7.598SerLeu: 7.598 ± 0.038	2.072SerMet: 2.072 ± 0.019	3.768SerAsn: 3.768 ± 0.031	3.789SerPro: 3.789 ± 0.038	3.051SerGln: 3.051 ± 0.024	4.799SerArg: 4.799 ± 0.036	9.311SerSer: 9.311 ± 0.071	5.707SerThr: 5.707 ± 0.046	5.46SerVal: 5.46 ± 0.036	0.908SerTrp: 0.908 ± 0.013	2.051SerTyr: 2.051 ± 0.019	0.004SerXaa: 0.004 ± 0.001
Thr	4.358ThrAla: 4.358 ± 0.03	1.043ThrCys: 1.043 ± 0.018	2.859ThrAsp: 2.859 ± 0.025	3.17ThrGlu: 3.17 ± 0.027	2.453ThrPhe: 2.453 ± 0.024	3.151ThrGly: 3.151 ± 0.04	1.306ThrHis: 1.306 ± 0.015	3.226ThrIle: 3.226 ± 0.03	3.266ThrLys: 3.266 ± 0.025	5.831ThrLeu: 5.831 ± 0.039	1.448ThrMet: 1.448 ± 0.018	2.474ThrAsn: 2.474 ± 0.022	2.993ThrPro: 2.993 ± 0.03	2.327ThrGln: 2.327 ± 0.022	3.17ThrArg: 3.17 ± 0.022	5.733ThrSer: 5.733 ± 0.046	3.997ThrThr: 3.997 ± 0.041	3.548ThrVal: 3.548 ± 0.031	0.651ThrTrp: 0.651 ± 0.011	1.455ThrTyr: 1.455 ± 0.016	0.002ThrXaa: 0.002 ± 0.001
Val	5.722ValAla: 5.722 ± 0.037	1.267ValCys: 1.267 ± 0.017	4.043ValAsp: 4.043 ± 0.027	4.383ValGlu: 4.383 ± 0.029	2.684ValPhe: 2.684 ± 0.025	3.511ValGly: 3.511 ± 0.029	1.641ValHis: 1.641 ± 0.018	3.31ValIle: 3.31 ± 0.026	3.688ValLys: 3.688 ± 0.027	6.685ValLeu: 6.685 ± 0.041	1.674ValMet: 1.674 ± 0.017	2.748ValAsn: 2.748 ± 0.029	2.861ValPro: 2.861 ± 0.02	2.799ValGln: 2.799 ± 0.022	3.681ValArg: 3.681 ± 0.027	5.381ValSer: 5.381 ± 0.04	3.982ValThr: 3.982 ± 0.039	5.039ValVal: 5.039 ± 0.032	0.743ValTrp: 0.743 ± 0.013	1.895ValTyr: 1.895 ± 0.022	0.003ValXaa: 0.003 ± 0.001
Trp	0.619TrpAla: 0.619 ± 0.012	0.206TrpCys: 0.206 ± 0.005	0.613TrpAsp: 0.613 ± 0.012	0.603TrpGlu: 0.603 ± 0.01	0.441TrpPhe: 0.441 ± 0.01	0.54TrpGly: 0.54 ± 0.011	0.308TrpHis: 0.308 ± 0.008	0.662TrpIle: 0.662 ± 0.011	0.821TrpLys: 0.821 ± 0.013	1.188TrpLeu: 1.188 ± 0.015	0.356TrpMet: 0.356 ± 0.007	0.592TrpAsn: 0.592 ± 0.011	0.347TrpPro: 0.347 ± 0.009	0.5TrpGln: 0.5 ± 0.01	0.785TrpArg: 0.785 ± 0.014	0.883TrpSer: 0.883 ± 0.014	0.7TrpThr: 0.7 ± 0.011	0.666TrpVal: 0.666 ± 0.011	0.171TrpTrp: 0.171 ± 0.006	0.348TrpTyr: 0.348 ± 0.007	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.013TyrAla: 2.013 ± 0.02	0.582TyrCys: 0.582 ± 0.014	1.64TyrAsp: 1.64 ± 0.017	1.681TyrGlu: 1.681 ± 0.017	1.249TyrPhe: 1.249 ± 0.018	1.685TyrGly: 1.685 ± 0.016	0.774TyrHis: 0.774 ± 0.012	1.323TyrIle: 1.323 ± 0.015	1.272TyrLys: 1.272 ± 0.015	2.631TyrLeu: 2.631 ± 0.026	0.704TyrMet: 0.704 ± 0.011	1.128TyrAsn: 1.128 ± 0.015	1.072TyrPro: 1.072 ± 0.014	1.136TyrGln: 1.136 ± 0.014	1.683TyrArg: 1.683 ± 0.017	1.982TyrSer: 1.982 ± 0.018	1.476TyrThr: 1.476 ± 0.019	1.89TyrVal: 1.89 ± 0.021	0.353TyrTrp: 0.353 ± 0.009	0.836TyrTyr: 0.836 ± 0.013	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.001XaaCys: 0.001 ± 0.0	0.002XaaAsp: 0.002 ± 0.001	0.001XaaGlu: 0.001 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.003XaaGly: 0.003 ± 0.001	0.002XaaHis: 0.002 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.002XaaLys: 0.002 ± 0.001	0.004XaaLeu: 0.004 ± 0.001	0.006XaaMet: 0.006 ± 0.001	0.001XaaAsn: 0.001 ± 0.0	0.002XaaPro: 0.002 ± 0.001	0.001XaaGln: 0.001 ± 0.0	0.003XaaArg: 0.003 ± 0.001	0.004XaaSer: 0.004 ± 0.001	0.001XaaThr: 0.001 ± 0.0	0.002XaaVal: 0.002 ± 0.0	0.001XaaTrp: 0.001 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.1XaaXaa: 0.1 ± 0.007

Statistics based on 15449 proteins (5873550 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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