Lettuce virus X

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3CJG4|B3CJG4_9VIRU Triple-gene-block-protein 2 OS=Lettuce virus X OX=447171 GN=TGBp2 PE=4 SV=1
MM1 pKa = 7.65AVPLLHH7 pKa = 7.29KK8 pKa = 10.64LLLEE12 pKa = 4.33NEE14 pKa = 4.57FQRR17 pKa = 11.84TATPLSYY24 pKa = 10.36PIIIFGTAGSGKK36 pKa = 6.1TTIIRR41 pKa = 11.84LLAAAYY47 pKa = 8.09PDD49 pKa = 3.62LHH51 pKa = 6.42FSSFRR56 pKa = 11.84PCLLLPNTRR65 pKa = 11.84RR66 pKa = 11.84SQVAANPLTPTDD78 pKa = 3.66VLDD81 pKa = 4.22EE82 pKa = 4.45FLAGPNPAVSLAKK95 pKa = 10.52FCDD98 pKa = 3.64PLQYY102 pKa = 11.22NCTDD106 pKa = 3.79LPLPHH111 pKa = 6.21YY112 pKa = 8.73TSNHH116 pKa = 5.0TYY118 pKa = 9.65RR119 pKa = 11.84FCPATCDD126 pKa = 4.18LLNQLFSTTLSSRR139 pKa = 11.84LPTAASITRR148 pKa = 11.84PDD150 pKa = 4.89PYY152 pKa = 11.09SQDD155 pKa = 3.23PSGTVVALEE164 pKa = 3.86QDD166 pKa = 3.54LLDD169 pKa = 3.64ILKK172 pKa = 9.58QHH174 pKa = 6.34GAFPKK179 pKa = 10.49KK180 pKa = 9.63PAEE183 pKa = 4.13LTGQTLPAVAFYY195 pKa = 10.78CSSLQDD201 pKa = 4.5AYY203 pKa = 10.99DD204 pKa = 4.47ADD206 pKa = 4.04PAATFIALTRR216 pKa = 11.84HH217 pKa = 4.4TTKK220 pKa = 11.09LEE222 pKa = 3.67IFEE225 pKa = 4.95LDD227 pKa = 3.42ARR229 pKa = 11.84PDD231 pKa = 3.43PTAA234 pKa = 4.44

Molecular weight: 25.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3CJG5|B3CJG5_9VIRU Movement protein TGBp3 OS=Lettuce virus X OX=447171 GN=TGBp3 PE=3 SV=1
MM1 pKa = 7.83PGLTPPPNHH10 pKa = 6.06EE11 pKa = 4.05HH12 pKa = 6.12TFQIIAIGFLCCGIIYY28 pKa = 10.38ALRR31 pKa = 11.84TNHH34 pKa = 6.59APHH37 pKa = 6.87TGDD40 pKa = 3.49QQHH43 pKa = 6.28SLPFGGHH50 pKa = 4.91YY51 pKa = 9.95QDD53 pKa = 3.09GTKK56 pKa = 10.02RR57 pKa = 11.84VIYY60 pKa = 10.01NSPSYY65 pKa = 11.06PNVRR69 pKa = 11.84CSKK72 pKa = 10.35LLALAAILGLSAFILGNQWYY92 pKa = 9.55SRR94 pKa = 11.84RR95 pKa = 11.84SVTRR99 pKa = 11.84IHH101 pKa = 6.15TCVHH105 pKa = 6.78CSNVPPSLL113 pKa = 3.68

Molecular weight: 12.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2381

84

1708

476.2

53.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.03 ± 1.134

1.428 ± 0.649

5.124 ± 0.337

4.704 ± 1.172

4.746 ± 0.343

4.662 ± 0.445

3.36 ± 0.558

4.284 ± 0.501

4.746 ± 1.196

9.366 ± 1.122

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.016 ± 0.437

4.62 ± 0.357

8.19 ± 0.835

4.116 ± 0.265

5.124 ± 0.192

6.888 ± 0.681

7.686 ± 0.771

5.67 ± 0.639

1.134 ± 0.356

3.108 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski