Corchorus golden mosaic virus
Average proteome isoelectric point is 8.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A5H144|A5H144_9GEMI AC4 protein OS=Corchorus golden mosaic virus OX=390436 GN=AC4 PE=3 SV=1
MM1 pKa = 7.56 SGRR4 pKa = 11.84 FKK6 pKa = 10.91 KK7 pKa = 10.43 QGVSFFLTWPKK18 pKa = 10.76 CPVTKK23 pKa = 10.45 EE24 pKa = 3.98 SALDD28 pKa = 3.55 QIQALTLPTNIVYY41 pKa = 10.07 IRR43 pKa = 11.84 VCEE46 pKa = 4.17 EE47 pKa = 3.49 KK48 pKa = 10.64 HH49 pKa = 6.14 QDD51 pKa = 3.54 GSPHH55 pKa = 5.97 LHH57 pKa = 6.9 ALVQFQKK64 pKa = 11.17 KK65 pKa = 9.05 FICTNCRR72 pKa = 11.84 LFDD75 pKa = 4.13 LSHH78 pKa = 6.67 PQNSRR83 pKa = 11.84 QFHH86 pKa = 5.18 CHH88 pKa = 5.9 IEE90 pKa = 4.25 TARR93 pKa = 11.84 SSSDD97 pKa = 2.64 AKK99 pKa = 11.09 SYY101 pKa = 10.6 IEE103 pKa = 4.15 KK104 pKa = 10.8 DD105 pKa = 3.55 GVFCEE110 pKa = 4.16 WGTFQVDD117 pKa = 3.02 GRR119 pKa = 11.84 SARR122 pKa = 11.84 GGQQTVNEE130 pKa = 4.71 AYY132 pKa = 10.35 AKK134 pKa = 10.48 ALNSGSKK141 pKa = 10.68 DD142 pKa = 3.19 EE143 pKa = 4.63 ALNIIKK149 pKa = 10.23 EE150 pKa = 4.48 LVPKK154 pKa = 10.49 DD155 pKa = 3.6 YY156 pKa = 11.31 VLQFHH161 pKa = 6.89 NLNQNLEE168 pKa = 4.45 RR169 pKa = 11.84 IFAPPVNVFEE179 pKa = 4.87 PPFPLSSFNNVPAVINQWVNDD200 pKa = 4.13 NIMDD204 pKa = 4.59 AAARR208 pKa = 11.84 PFRR211 pKa = 11.84 PISIIIEE218 pKa = 4.42 GPSRR222 pKa = 11.84 TGKK225 pKa = 7.64 TLWARR230 pKa = 11.84 SLGRR234 pKa = 11.84 HH235 pKa = 5.8 NYY237 pKa = 10.07 LCGHH241 pKa = 7.41 LDD243 pKa = 4.18 LSPKK247 pKa = 10.02 VYY249 pKa = 10.89 SNEE252 pKa = 3.02 AWYY255 pKa = 10.82 NVIDD259 pKa = 5.29 DD260 pKa = 4.41 VDD262 pKa = 3.64 PHH264 pKa = 6.13 YY265 pKa = 10.52 LKK267 pKa = 10.88 HH268 pKa = 5.92 MKK270 pKa = 10.16 EE271 pKa = 4.06 FMGAQRR277 pKa = 11.84 DD278 pKa = 3.71 WQSNCKK284 pKa = 9.1 YY285 pKa = 10.44 GKK287 pKa = 9.33 PIQINGGIPTIFLCNPGPTSSYY309 pKa = 10.97 KK310 pKa = 10.65 EE311 pKa = 3.81 FFEE314 pKa = 4.36 EE315 pKa = 4.53 EE316 pKa = 3.9 KK317 pKa = 11.18 NKK319 pKa = 10.76 AINDD323 pKa = 3.83 WAKK326 pKa = 11.0 KK327 pKa = 8.69 NVIYY331 pKa = 9.24 VTIEE335 pKa = 3.68 EE336 pKa = 4.69 PFFNTTNQEE345 pKa = 3.9 STSALEE351 pKa = 4.14 EE352 pKa = 4.68 SNSSEE357 pKa = 4.36 TNN359 pKa = 3.08
Molecular weight: 40.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.168
IPC2_protein 6.173
IPC_protein 6.262
Toseland 6.491
ProMoST 6.664
Dawson 6.634
Bjellqvist 6.605
Wikipedia 6.649
Rodwell 6.62
Grimsley 6.605
Solomon 6.634
Lehninger 6.634
Nozaki 6.912
DTASelect 7.059
Thurlkill 7.088
EMBOSS 7.073
Sillero 7.029
Patrickios 4.139
IPC_peptide 6.649
IPC2_peptide 6.839
IPC2.peptide.svr19 6.799
Protein with the highest isoelectric point:
>tr|A5H141|A5H141_9GEMI Replication enhancer OS=Corchorus golden mosaic virus OX=390436 GN=AC3 PE=3 SV=1
MM1 pKa = 7.19 KK2 pKa = 10.37 RR3 pKa = 11.84 EE4 pKa = 4.22 APWRR8 pKa = 11.84 TNAGTSKK15 pKa = 10.08 VRR17 pKa = 11.84 RR18 pKa = 11.84 ALNFSPRR25 pKa = 11.84 SGLGPKK31 pKa = 10.13 ASAWVNRR38 pKa = 11.84 PMYY41 pKa = 9.63 RR42 pKa = 11.84 KK43 pKa = 8.55 PRR45 pKa = 11.84 IYY47 pKa = 9.41 RR48 pKa = 11.84 TYY50 pKa = 10.72 RR51 pKa = 11.84 SPDD54 pKa = 3.24 VPKK57 pKa = 10.81 GCEE60 pKa = 4.39 GPCKK64 pKa = 10.23 VQSFEE69 pKa = 3.82 QRR71 pKa = 11.84 HH72 pKa = 6.26 DD73 pKa = 3.16 ISHH76 pKa = 6.13 VGKK79 pKa = 10.55 VMCISDD85 pKa = 3.56 VTRR88 pKa = 11.84 GNGITHH94 pKa = 6.78 RR95 pKa = 11.84 VGKK98 pKa = 9.62 RR99 pKa = 11.84 FCIKK103 pKa = 10.12 SVYY106 pKa = 10.1 ILGKK110 pKa = 10.11 VWMDD114 pKa = 4.51 DD115 pKa = 3.58 NIKK118 pKa = 10.64 LKK120 pKa = 10.74 NHH122 pKa = 5.99 TNSVMFWLVRR132 pKa = 11.84 DD133 pKa = 3.7 RR134 pKa = 11.84 RR135 pKa = 11.84 PYY137 pKa = 8.05 GTPMDD142 pKa = 4.6 FGQVFNMYY150 pKa = 10.66 DD151 pKa = 3.86 NEE153 pKa = 4.36 PSTATIKK160 pKa = 10.89 NDD162 pKa = 3.0 LRR164 pKa = 11.84 DD165 pKa = 3.73 RR166 pKa = 11.84 YY167 pKa = 9.95 QVLHH171 pKa = 6.65 RR172 pKa = 11.84 FASKK176 pKa = 9.07 VTGGQYY182 pKa = 11.09 ASNEE186 pKa = 3.76 QSLVRR191 pKa = 11.84 RR192 pKa = 11.84 FWKK195 pKa = 10.44 VNNHH199 pKa = 4.48 VVYY202 pKa = 10.66 NHH204 pKa = 5.91 QEE206 pKa = 3.39 AAKK209 pKa = 10.14 YY210 pKa = 10.5 DD211 pKa = 3.75 NHH213 pKa = 6.5 TEE215 pKa = 4.02 NALLLYY221 pKa = 7.29 MACTHH226 pKa = 7.07 ASNPVYY232 pKa = 9.86 ATLKK236 pKa = 9.47 IRR238 pKa = 11.84 IYY240 pKa = 10.59 FYY242 pKa = 11.32 DD243 pKa = 4.06 SISNN247 pKa = 3.66
Molecular weight: 28.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.268
IPC2_protein 9.531
IPC_protein 9.736
Toseland 10.028
ProMoST 9.794
Dawson 10.262
Bjellqvist 9.984
Wikipedia 10.467
Rodwell 10.584
Grimsley 10.35
Solomon 10.292
Lehninger 10.248
Nozaki 10.072
DTASelect 9.97
Thurlkill 10.116
EMBOSS 10.452
Sillero 10.189
Patrickios 9.911
IPC_peptide 10.292
IPC2_peptide 8.873
IPC2.peptide.svr19 8.487
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1554
100
359
222.0
25.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.084 ± 0.595
2.124 ± 0.208
4.762 ± 0.274
4.44 ± 0.734
4.376 ± 0.492
4.955 ± 0.648
3.475 ± 0.377
5.92 ± 0.492
5.792 ± 0.392
7.014 ± 0.556
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.252 ± 0.415
6.306 ± 0.579
5.084 ± 0.387
3.925 ± 0.404
7.658 ± 1.066
10.039 ± 1.022
5.212 ± 0.499
6.306 ± 0.633
1.351 ± 0.247
3.925 ± 0.668
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here