Amino acid dipepetide frequency for Nostoc sp. Peltigera malacea cyanobiont DB3992

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.208AlaAla: 7.208 ± 0.09	0.806AlaCys: 0.806 ± 0.021	3.932AlaAsp: 3.932 ± 0.044	5.063AlaGlu: 5.063 ± 0.061	2.965AlaPhe: 2.965 ± 0.047	5.275AlaGly: 5.275 ± 0.065	1.271AlaHis: 1.271 ± 0.027	6.85AlaIle: 6.85 ± 0.072	4.196AlaLys: 4.196 ± 0.061	8.278AlaLeu: 8.278 ± 0.076	1.59AlaMet: 1.59 ± 0.031	3.578AlaAsn: 3.578 ± 0.059	2.727AlaPro: 2.727 ± 0.043	3.967AlaGln: 3.967 ± 0.056	3.456AlaArg: 3.456 ± 0.051	4.869AlaSer: 4.869 ± 0.056	4.709AlaThr: 4.709 ± 0.053	5.507AlaVal: 5.507 ± 0.054	1.017AlaTrp: 1.017 ± 0.024	2.393AlaTyr: 2.393 ± 0.041	0.001AlaXaa: 0.001 ± 0.001
Cys	0.608CysAla: 0.608 ± 0.02	0.16CysCys: 0.16 ± 0.01	0.554CysAsp: 0.554 ± 0.019	0.527CysGlu: 0.527 ± 0.019	0.425CysPhe: 0.425 ± 0.016	0.781CysGly: 0.781 ± 0.023	0.274CysHis: 0.274 ± 0.012	0.613CysIle: 0.613 ± 0.021	0.382CysLys: 0.382 ± 0.016	1.117CysLeu: 1.117 ± 0.029	0.168CysMet: 0.168 ± 0.011	0.422CysAsn: 0.422 ± 0.017	0.493CysPro: 0.493 ± 0.018	0.548CysGln: 0.548 ± 0.018	0.498CysArg: 0.498 ± 0.019	0.618CysSer: 0.618 ± 0.02	0.479CysThr: 0.479 ± 0.018	0.58CysVal: 0.58 ± 0.016	0.153CysTrp: 0.153 ± 0.011	0.356CysTyr: 0.356 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.635AspAla: 3.635 ± 0.052	0.492AspCys: 0.492 ± 0.018	2.274AspAsp: 2.274 ± 0.043	3.089AspGlu: 3.089 ± 0.046	2.418AspPhe: 2.418 ± 0.043	3.282AspGly: 3.282 ± 0.058	0.649AspHis: 0.649 ± 0.022	3.612AspIle: 3.612 ± 0.053	2.39AspLys: 2.39 ± 0.046	5.525AspLeu: 5.525 ± 0.059	0.743AspMet: 0.743 ± 0.022	2.064AspAsn: 2.064 ± 0.036	2.189AspPro: 2.189 ± 0.038	1.56AspGln: 1.56 ± 0.027	3.243AspArg: 3.243 ± 0.052	3.084AspSer: 3.084 ± 0.041	2.546AspThr: 2.546 ± 0.041	3.05AspVal: 3.05 ± 0.042	0.897AspTrp: 0.897 ± 0.024	1.928AspTyr: 1.928 ± 0.035	0.001AspXaa: 0.001 ± 0.001
Glu	5.326GluAla: 5.326 ± 0.06	0.513GluCys: 0.513 ± 0.019	2.8GluAsp: 2.8 ± 0.047	4.138GluGlu: 4.138 ± 0.062	2.51GluPhe: 2.51 ± 0.038	3.202GluGly: 3.202 ± 0.05	1.078GluHis: 1.078 ± 0.027	5.032GluIle: 5.032 ± 0.06	3.559GluLys: 3.559 ± 0.05	7.091GluLeu: 7.091 ± 0.076	1.286GluMet: 1.286 ± 0.03	2.873GluAsn: 2.873 ± 0.047	2.296GluPro: 2.296 ± 0.039	3.721GluGln: 3.721 ± 0.047	3.425GluArg: 3.425 ± 0.05	3.502GluSer: 3.502 ± 0.045	3.447GluThr: 3.447 ± 0.052	4.431GluVal: 4.431 ± 0.055	0.795GluTrp: 0.795 ± 0.021	2.008GluTyr: 2.008 ± 0.037	0.001GluXaa: 0.001 ± 0.001
Phe	3.114PheAla: 3.114 ± 0.05	0.51PheCys: 0.51 ± 0.016	2.258PheAsp: 2.258 ± 0.038	2.291PheGlu: 2.291 ± 0.039	1.749PhePhe: 1.749 ± 0.039	2.919PheGly: 2.919 ± 0.041	0.739PheHis: 0.739 ± 0.021	2.49PheIle: 2.49 ± 0.04	1.659PheLys: 1.659 ± 0.034	4.214PheLeu: 4.214 ± 0.054	0.693PheMet: 0.693 ± 0.018	1.879PheAsn: 1.879 ± 0.037	1.813PhePro: 1.813 ± 0.027	1.887PheGln: 1.887 ± 0.037	1.768PheArg: 1.768 ± 0.027	3.067PheSer: 3.067 ± 0.039	2.436PheThr: 2.436 ± 0.036	2.604PheVal: 2.604 ± 0.042	0.66PheTrp: 0.66 ± 0.022	1.453PheTyr: 1.453 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	4.828GlyAla: 4.828 ± 0.073	0.759GlyCys: 0.759 ± 0.022	3.288GlyAsp: 3.288 ± 0.045	4.03GlyGlu: 4.03 ± 0.042	2.904GlyPhe: 2.904 ± 0.04	4.817GlyGly: 4.817 ± 0.066	1.187GlyHis: 1.187 ± 0.028	5.235GlyIle: 5.235 ± 0.061	4.133GlyLys: 4.133 ± 0.053	6.802GlyLeu: 6.802 ± 0.064	1.414GlyMet: 1.414 ± 0.033	3.258GlyAsn: 3.258 ± 0.062	1.24GlyPro: 1.24 ± 0.031	2.84GlyGln: 2.84 ± 0.045	3.147GlyArg: 3.147 ± 0.053	4.145GlySer: 4.145 ± 0.062	3.829GlyThr: 3.829 ± 0.052	4.738GlyVal: 4.738 ± 0.063	1.093GlyTrp: 1.093 ± 0.025	2.319GlyTyr: 2.319 ± 0.039	0.001GlyXaa: 0.001 ± 0.001
His	1.02HisAla: 1.02 ± 0.023	0.264HisCys: 0.264 ± 0.013	0.741HisAsp: 0.741 ± 0.02	0.998HisGlu: 0.998 ± 0.024	0.812HisPhe: 0.812 ± 0.023	1.043HisGly: 1.043 ± 0.024	0.578HisHis: 0.578 ± 0.024	1.2HisIle: 1.2 ± 0.025	0.775HisLys: 0.775 ± 0.022	2.262HisLeu: 2.262 ± 0.038	0.221HisMet: 0.221 ± 0.011	0.757HisAsn: 0.757 ± 0.025	1.257HisPro: 1.257 ± 0.028	1.114HisGln: 1.114 ± 0.027	1.097HisArg: 1.097 ± 0.027	1.17HisSer: 1.17 ± 0.028	0.949HisThr: 0.949 ± 0.026	0.789HisVal: 0.789 ± 0.024	0.369HisTrp: 0.369 ± 0.015	0.691HisTyr: 0.691 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.129IleAla: 7.129 ± 0.068	0.748IleCys: 0.748 ± 0.023	3.769IleAsp: 3.769 ± 0.055	4.417IleGlu: 4.417 ± 0.055	2.713IlePhe: 2.713 ± 0.044	4.546IleGly: 4.546 ± 0.051	1.273IleHis: 1.273 ± 0.03	3.932IleIle: 3.932 ± 0.052	3.224IleLys: 3.224 ± 0.047	7.077IleLeu: 7.077 ± 0.071	0.885IleMet: 0.885 ± 0.022	3.212IleAsn: 3.212 ± 0.054	3.617IlePro: 3.617 ± 0.045	3.178IleGln: 3.178 ± 0.042	3.152IleArg: 3.152 ± 0.046	4.694IleSer: 4.694 ± 0.062	3.89IleThr: 3.89 ± 0.054	4.408IleVal: 4.408 ± 0.047	0.905IleTrp: 0.905 ± 0.021	2.081IleTyr: 2.081 ± 0.037	0.0IleXaa: 0.0 ± 0.0
Lys	4.017LysAla: 4.017 ± 0.049	0.347LysCys: 0.347 ± 0.016	2.301LysAsp: 2.301 ± 0.041	2.844LysGlu: 2.844 ± 0.041	1.924LysPhe: 1.924 ± 0.04	2.806LysGly: 2.806 ± 0.048	0.875LysHis: 0.875 ± 0.025	3.726LysIle: 3.726 ± 0.054	2.381LysLys: 2.381 ± 0.044	5.703LysLeu: 5.703 ± 0.057	0.978LysMet: 0.978 ± 0.024	2.256LysAsn: 2.256 ± 0.046	2.49LysPro: 2.49 ± 0.043	2.755LysGln: 2.755 ± 0.046	2.385LysArg: 2.385 ± 0.036	3.212LysSer: 3.212 ± 0.048	3.026LysThr: 3.026 ± 0.047	3.304LysVal: 3.304 ± 0.045	0.554LysTrp: 0.554 ± 0.017	1.555LysTyr: 1.555 ± 0.034	0.001LysXaa: 0.001 ± 0.001
Leu	9.434LeuAla: 9.434 ± 0.081	1.028LeuCys: 1.028 ± 0.028	5.237LeuAsp: 5.237 ± 0.061	7.396LeuGlu: 7.396 ± 0.079	3.874LeuPhe: 3.874 ± 0.051	7.607LeuGly: 7.607 ± 0.076	1.928LeuHis: 1.928 ± 0.034	6.915LeuIle: 6.915 ± 0.068	5.775LeuLys: 5.775 ± 0.065	11.649LeuLeu: 11.649 ± 0.111	2.046LeuMet: 2.046 ± 0.037	4.818LeuAsn: 4.818 ± 0.057	5.636LeuPro: 5.636 ± 0.063	5.8LeuGln: 5.8 ± 0.074	5.56LeuArg: 5.56 ± 0.06	7.483LeuSer: 7.483 ± 0.076	6.447LeuThr: 6.447 ± 0.068	7.295LeuVal: 7.295 ± 0.074	1.372LeuTrp: 1.372 ± 0.03	2.857LeuTyr: 2.857 ± 0.041	0.001LeuXaa: 0.001 ± 0.001
Met	1.567MetAla: 1.567 ± 0.037	0.114MetCys: 0.114 ± 0.009	0.705MetAsp: 0.705 ± 0.018	0.961MetGlu: 0.961 ± 0.024	0.562MetPhe: 0.562 ± 0.018	1.224MetGly: 1.224 ± 0.03	0.328MetHis: 0.328 ± 0.013	1.085MetIle: 1.085 ± 0.025	0.982MetLys: 0.982 ± 0.024	1.91MetLeu: 1.91 ± 0.035	0.395MetMet: 0.395 ± 0.015	0.88MetAsn: 0.88 ± 0.026	0.952MetPro: 0.952 ± 0.025	0.917MetGln: 0.917 ± 0.023	0.956MetArg: 0.956 ± 0.023	1.352MetSer: 1.352 ± 0.027	1.336MetThr: 1.336 ± 0.027	1.185MetVal: 1.185 ± 0.028	0.158MetTrp: 0.158 ± 0.009	0.357MetTyr: 0.357 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.063AsnAla: 3.063 ± 0.051	0.472AsnCys: 0.472 ± 0.019	1.928AsnAsp: 1.928 ± 0.043	2.035AsnGlu: 2.035 ± 0.037	2.107AsnPhe: 2.107 ± 0.038	2.853AsnGly: 2.853 ± 0.055	0.912AsnHis: 0.912 ± 0.025	3.051AsnIle: 3.051 ± 0.047	1.87AsnLys: 1.87 ± 0.039	5.813AsnLeu: 5.813 ± 0.073	0.647AsnMet: 0.647 ± 0.019	2.242AsnAsn: 2.242 ± 0.048	2.925AsnPro: 2.925 ± 0.046	2.745AsnGln: 2.745 ± 0.046	2.384AsnArg: 2.384 ± 0.036	3.276AsnSer: 3.276 ± 0.051	2.437AsnThr: 2.437 ± 0.041	2.292AsnVal: 2.292 ± 0.037	0.771AsnTrp: 0.771 ± 0.023	1.639AsnTyr: 1.639 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	3.138ProAla: 3.138 ± 0.047	0.318ProCys: 0.318 ± 0.016	2.925ProAsp: 2.925 ± 0.044	3.793ProGlu: 3.793 ± 0.047	1.732ProPhe: 1.732 ± 0.035	2.887ProGly: 2.887 ± 0.045	0.852ProHis: 0.852 ± 0.022	3.097ProIle: 3.097 ± 0.043	2.261ProLys: 2.261 ± 0.038	4.649ProLeu: 4.649 ± 0.057	0.739ProMet: 0.739 ± 0.021	2.385ProAsn: 2.385 ± 0.044	2.191ProPro: 2.191 ± 0.048	2.52ProGln: 2.52 ± 0.043	1.673ProArg: 1.673 ± 0.034	2.924ProSer: 2.924 ± 0.049	2.876ProThr: 2.876 ± 0.05	3.1ProVal: 3.1 ± 0.048	0.552ProTrp: 0.552 ± 0.022	1.3ProTyr: 1.3 ± 0.028	0.001ProXaa: 0.001 ± 0.001
Gln	4.487GlnAla: 4.487 ± 0.062	0.339GlnCys: 0.339 ± 0.015	2.09GlnAsp: 2.09 ± 0.036	3.695GlnGlu: 3.695 ± 0.048	1.764GlnPhe: 1.764 ± 0.034	3.225GlnGly: 3.225 ± 0.052	0.862GlnHis: 0.862 ± 0.024	3.754GlnIle: 3.754 ± 0.049	2.852GlnLys: 2.852 ± 0.037	6.024GlnLeu: 6.024 ± 0.068	1.092GlnMet: 1.092 ± 0.026	2.21GlnAsn: 2.21 ± 0.038	2.61GlnPro: 2.61 ± 0.047	3.745GlnGln: 3.745 ± 0.061	2.853GlnArg: 2.853 ± 0.048	2.994GlnSer: 2.994 ± 0.046	2.928GlnThr: 2.928 ± 0.042	3.809GlnVal: 3.809 ± 0.055	0.677GlnTrp: 0.677 ± 0.019	1.276GlnTyr: 1.276 ± 0.03	0.002GlnXaa: 0.002 ± 0.001
Arg	3.14ArgAla: 3.14 ± 0.047	0.518ArgCys: 0.518 ± 0.018	2.53ArgAsp: 2.53 ± 0.042	3.291ArgGlu: 3.291 ± 0.048	2.206ArgPhe: 2.206 ± 0.037	2.955ArgGly: 2.955 ± 0.043	1.016ArgHis: 1.016 ± 0.026	3.315ArgIle: 3.315 ± 0.049	2.219ArgLys: 2.219 ± 0.037	5.949ArgLeu: 5.949 ± 0.068	0.946ArgMet: 0.946 ± 0.024	2.148ArgAsn: 2.148 ± 0.034	1.96ArgPro: 1.96 ± 0.03	3.21ArgGln: 3.21 ± 0.044	2.996ArgArg: 2.996 ± 0.055	3.241ArgSer: 3.241 ± 0.04	2.518ArgThr: 2.518 ± 0.041	3.43ArgVal: 3.43 ± 0.053	0.786ArgTrp: 0.786 ± 0.024	1.892ArgTyr: 1.892 ± 0.037	0.001ArgXaa: 0.001 ± 0.001
Ser	4.575SerAla: 4.575 ± 0.059	0.633SerCys: 0.633 ± 0.021	3.298SerAsp: 3.298 ± 0.041	3.898SerGlu: 3.898 ± 0.055	2.64SerPhe: 2.64 ± 0.038	4.788SerGly: 4.788 ± 0.056	1.258SerHis: 1.258 ± 0.028	3.986SerIle: 3.986 ± 0.056	2.901SerLys: 2.901 ± 0.046	7.531SerLeu: 7.531 ± 0.073	1.182SerMet: 1.182 ± 0.028	3.01SerAsn: 3.01 ± 0.044	3.317SerPro: 3.317 ± 0.054	3.679SerGln: 3.679 ± 0.043	3.189SerArg: 3.189 ± 0.044	4.622SerSer: 4.622 ± 0.057	3.662SerThr: 3.662 ± 0.046	4.12SerVal: 4.12 ± 0.055	0.924SerTrp: 0.924 ± 0.025	1.934SerTyr: 1.934 ± 0.033	0.001SerXaa: 0.001 ± 0.001
Thr	4.694ThrAla: 4.694 ± 0.057	0.491ThrCys: 0.491 ± 0.017	2.668ThrAsp: 2.668 ± 0.038	3.39ThrGlu: 3.39 ± 0.05	2.246ThrPhe: 2.246 ± 0.037	4.246ThrGly: 4.246 ± 0.054	0.988ThrHis: 0.988 ± 0.026	3.833ThrIle: 3.833 ± 0.052	2.488ThrLys: 2.488 ± 0.043	6.336ThrLeu: 6.336 ± 0.059	0.791ThrMet: 0.791 ± 0.02	2.477ThrAsn: 2.477 ± 0.046	3.472ThrPro: 3.472 ± 0.059	2.961ThrGln: 2.961 ± 0.047	2.405ThrArg: 2.405 ± 0.04	3.735ThrSer: 3.735 ± 0.052	3.514ThrThr: 3.514 ± 0.056	3.938ThrVal: 3.938 ± 0.048	0.712ThrTrp: 0.712 ± 0.019	1.625ThrTyr: 1.625 ± 0.033	0.001ThrXaa: 0.001 ± 0.001
Val	5.651ValAla: 5.651 ± 0.053	0.689ValCys: 0.689 ± 0.021	3.276ValAsp: 3.276 ± 0.048	4.471ValGlu: 4.471 ± 0.05	2.611ValPhe: 2.611 ± 0.04	4.497ValGly: 4.497 ± 0.057	1.02ValHis: 1.02 ± 0.026	4.428ValIle: 4.428 ± 0.048	3.4ValLys: 3.4 ± 0.041	6.696ValLeu: 6.696 ± 0.066	1.346ValMet: 1.346 ± 0.028	3.012ValAsn: 3.012 ± 0.043	2.851ValPro: 2.851 ± 0.044	2.984ValGln: 2.984 ± 0.044	3.264ValArg: 3.264 ± 0.047	4.309ValSer: 4.309 ± 0.048	3.809ValThr: 3.809 ± 0.049	4.81ValVal: 4.81 ± 0.059	0.856ValTrp: 0.856 ± 0.024	1.915ValTyr: 1.915 ± 0.032	0.001ValXaa: 0.001 ± 0.001
Trp	0.869TrpAla: 0.869 ± 0.023	0.144TrpCys: 0.144 ± 0.01	0.629TrpAsp: 0.629 ± 0.021	0.904TrpGlu: 0.904 ± 0.024	0.625TrpPhe: 0.625 ± 0.018	0.962TrpGly: 0.962 ± 0.031	0.331TrpHis: 0.331 ± 0.014	0.853TrpIle: 0.853 ± 0.023	0.617TrpLys: 0.617 ± 0.018	1.842TrpLeu: 1.842 ± 0.036	0.322TrpMet: 0.322 ± 0.014	0.646TrpAsn: 0.646 ± 0.021	0.268TrpPro: 0.268 ± 0.013	1.166TrpGln: 1.166 ± 0.032	0.864TrpArg: 0.864 ± 0.021	0.812TrpSer: 0.812 ± 0.023	0.598TrpThr: 0.598 ± 0.019	0.923TrpVal: 0.923 ± 0.025	0.233TrpTrp: 0.233 ± 0.012	0.448TrpTyr: 0.448 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.158TyrAla: 2.158 ± 0.036	0.411TyrCys: 0.411 ± 0.017	1.469TyrAsp: 1.469 ± 0.03	1.801TyrGlu: 1.801 ± 0.037	1.414TyrPhe: 1.414 ± 0.033	2.091TyrGly: 2.091 ± 0.038	0.69TyrHis: 0.69 ± 0.02	1.842TyrIle: 1.842 ± 0.034	1.376TyrLys: 1.376 ± 0.029	3.691TyrLeu: 3.691 ± 0.049	0.442TyrMet: 0.442 ± 0.016	1.334TyrAsn: 1.334 ± 0.032	1.608TyrPro: 1.608 ± 0.039	1.991TyrGln: 1.991 ± 0.041	1.951TyrArg: 1.951 ± 0.033	2.038TyrSer: 2.038 ± 0.036	1.575TyrThr: 1.575 ± 0.031	1.58TyrVal: 1.58 ± 0.028	0.568TyrTrp: 0.568 ± 0.019	1.111TyrTyr: 1.111 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.001XaaTyr: 0.001 ± 0.001	0.003XaaXaa: 0.003 ± 0.002

Statistics based on 6320 proteins (1697874 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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