Acinetobacter phage vB_AbaM_B9
Average proteome isoelectric point is 5.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 156 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S1GTZ6|A0A2S1GTZ6_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaM_B9 OX=2163899 GN=AB9_142 PE=4 SV=1
MM1 pKa = 7.21 SVNYY5 pKa = 10.34 SSVILIGADD14 pKa = 3.39 YY15 pKa = 11.22 DD16 pKa = 3.99 SLTWNTLTDD25 pKa = 3.61 EE26 pKa = 4.57 AKK28 pKa = 10.57 SIIRR32 pKa = 11.84 EE33 pKa = 4.06 EE34 pKa = 4.18 VLQDD38 pKa = 4.31 SPICYY43 pKa = 9.8 EE44 pKa = 3.76 WDD46 pKa = 3.76 DD47 pKa = 4.14 EE48 pKa = 4.38 NSEE51 pKa = 3.99 ITNQDD56 pKa = 3.03 EE57 pKa = 4.76 VEE59 pKa = 4.32 EE60 pKa = 4.2 AWEE63 pKa = 4.09 EE64 pKa = 3.77 QCIYY68 pKa = 10.4 EE69 pKa = 4.03 WEE71 pKa = 4.13 DD72 pKa = 4.83 RR73 pKa = 11.84 KK74 pKa = 11.24 GEE76 pKa = 4.14 WINDD80 pKa = 3.29 YY81 pKa = 10.89 FGFSGTIGNYY91 pKa = 5.6 FTGVIDD97 pKa = 3.76 YY98 pKa = 10.8 VGVEE102 pKa = 3.91 IHH104 pKa = 7.24 DD105 pKa = 4.8 LNADD109 pKa = 3.43 HH110 pKa = 6.98 QGAIDD115 pKa = 3.41 EE116 pKa = 4.5 WFKK119 pKa = 11.57 SFTVKK124 pKa = 10.63 PEE126 pKa = 3.76 LHH128 pKa = 6.71 HH129 pKa = 7.19 GVLVWW134 pKa = 3.61
Molecular weight: 15.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.769
IPC2_protein 3.897
IPC_protein 3.846
Toseland 3.668
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.681
Grimsley 3.579
Solomon 3.808
Lehninger 3.77
Nozaki 3.935
DTASelect 4.101
Thurlkill 3.706
EMBOSS 3.732
Sillero 3.973
Patrickios 0.744
IPC_peptide 3.808
IPC2_peptide 3.948
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|A0A2S1GU89|A0A2S1GU89_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaM_B9 OX=2163899 GN=AB9_091 PE=4 SV=1
MM1 pKa = 7.4 EE2 pKa = 5.23 KK3 pKa = 10.38 EE4 pKa = 4.0 IVVDD8 pKa = 3.85 EE9 pKa = 4.59 RR10 pKa = 11.84 MAFNNGRR17 pKa = 11.84 NYY19 pKa = 11.35 DD20 pKa = 4.53 DD21 pKa = 4.62 INTILISNWYY31 pKa = 7.17 NSKK34 pKa = 9.69 WKK36 pKa = 10.03 RR37 pKa = 11.84 QRR39 pKa = 11.84 KK40 pKa = 8.76 NNTVCFRR47 pKa = 11.84 SKK49 pKa = 11.17 LNWLYY54 pKa = 11.5
Molecular weight: 6.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.134
IPC2_protein 8.99
IPC_protein 9.048
Toseland 9.604
ProMoST 9.472
Dawson 9.853
Bjellqvist 9.575
Wikipedia 10.028
Rodwell 10.131
Grimsley 9.926
Solomon 9.94
Lehninger 9.911
Nozaki 9.663
DTASelect 9.545
Thurlkill 9.692
EMBOSS 10.014
Sillero 9.794
Patrickios 7.6
IPC_peptide 9.94
IPC2_peptide 8.302
IPC2.peptide.svr19 7.974
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
156
0
156
26605
47
863
170.5
19.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.905 ± 0.221
1.466 ± 0.125
6.721 ± 0.175
7.57 ± 0.298
4.544 ± 0.16
5.852 ± 0.236
1.797 ± 0.105
7.033 ± 0.163
8.066 ± 0.265
8.438 ± 0.201
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.214 ± 0.102
6.127 ± 0.222
2.454 ± 0.148
3.45 ± 0.132
3.714 ± 0.158
6.566 ± 0.209
5.732 ± 0.261
6.984 ± 0.179
1.376 ± 0.079
4.992 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here