Streptococcus satellite phage Javan752
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6A0I8|A0A4D6A0I8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan752 OX=2558847 GN=JavanS752_0014 PE=4 SV=1
MM1 pKa = 8.09 DD2 pKa = 5.18 NLAQHH7 pKa = 6.11 VGEE10 pKa = 4.66 KK11 pKa = 10.18 IKK13 pKa = 10.56 FFRR16 pKa = 11.84 KK17 pKa = 9.17 EE18 pKa = 3.97 NGWTQSVLAEE28 pKa = 4.2 KK29 pKa = 10.27 MSTSKK34 pKa = 8.64 QTISKK39 pKa = 9.22 YY40 pKa = 9.45 EE41 pKa = 3.68 KK42 pKa = 10.78 GIIKK46 pKa = 10.09 VNQDD50 pKa = 4.02 TIFKK54 pKa = 9.93 LADD57 pKa = 3.16 IFGVSIDD64 pKa = 3.96 SFFPSIIEE72 pKa = 4.48 EE73 pKa = 4.11 IATTSPIQSIYY84 pKa = 10.88 DD85 pKa = 3.27 QLTPPRR91 pKa = 11.84 QEE93 pKa = 3.78 KK94 pKa = 10.64 ALTYY98 pKa = 10.35 LKK100 pKa = 10.55 KK101 pKa = 10.37 QLLEE105 pKa = 4.02 QKK107 pKa = 10.32 NEE109 pKa = 4.21 NIVSEE114 pKa = 4.5 NIISLDD120 pKa = 3.66 DD121 pKa = 3.6 YY122 pKa = 11.31 RR123 pKa = 11.84 EE124 pKa = 4.27 SKK126 pKa = 8.86 TLPVIGVVTAGNGITQDD143 pKa = 4.14 DD144 pKa = 4.47 NLNMEE149 pKa = 4.13 KK150 pKa = 10.39 CFYY153 pKa = 10.23 TDD155 pKa = 5.38 EE156 pKa = 5.49 IPDD159 pKa = 4.38 DD160 pKa = 4.3 YY161 pKa = 11.82 DD162 pKa = 5.57 AIAYY166 pKa = 8.54 VVGNSMEE173 pKa = 4.18 PKK175 pKa = 9.97 IKK177 pKa = 10.88 NGDD180 pKa = 3.4 YY181 pKa = 11.13 LFIKK185 pKa = 8.35 NTPQVDD191 pKa = 3.97 YY192 pKa = 8.82 NTIGIFQVDD201 pKa = 3.58 GANYY205 pKa = 9.54 VKK207 pKa = 10.81 KK208 pKa = 10.47 LRR210 pKa = 11.84 QGYY213 pKa = 9.62 LEE215 pKa = 4.22 SLNPEE220 pKa = 4.27 CADD223 pKa = 3.24 IQLDD227 pKa = 3.77 EE228 pKa = 5.74 DD229 pKa = 3.79 NDD231 pKa = 3.21 IRR233 pKa = 11.84 TIGEE237 pKa = 3.96 VVSIYY242 pKa = 10.26 RR243 pKa = 11.84 EE244 pKa = 3.91 KK245 pKa = 11.19
Molecular weight: 27.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.658
IPC2_protein 4.647
IPC_protein 4.571
Toseland 4.406
ProMoST 4.685
Dawson 4.52
Bjellqvist 4.673
Wikipedia 4.406
Rodwell 4.406
Grimsley 4.317
Solomon 4.52
Lehninger 4.469
Nozaki 4.622
DTASelect 4.8
Thurlkill 4.406
EMBOSS 4.418
Sillero 4.685
Patrickios 3.935
IPC_peptide 4.52
IPC2_peptide 4.673
IPC2.peptide.svr19 4.631
Protein with the highest isoelectric point:
>tr|A0A4D5ZVP1|A0A4D5ZVP1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan752 OX=2558847 GN=JavanS752_0013 PE=4 SV=1
MM1 pKa = 7.25 KK2 pKa = 10.36 RR3 pKa = 11.84 IATIFLLGLPKK14 pKa = 9.96 VPKK17 pKa = 8.88 MRR19 pKa = 11.84 PIAYY23 pKa = 8.55 PRR25 pKa = 11.84 ATISNNPEE33 pKa = 3.14 ARR35 pKa = 11.84 GIYY38 pKa = 10.11 LEE40 pKa = 4.06 NKK42 pKa = 9.02 KK43 pKa = 10.62
Molecular weight: 4.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.286
IPC2_protein 10.058
IPC_protein 10.716
Toseland 10.935
ProMoST 10.657
Dawson 11.023
Bjellqvist 10.687
Wikipedia 11.199
Rodwell 11.418
Grimsley 11.052
Solomon 11.125
Lehninger 11.096
Nozaki 10.906
DTASelect 10.687
Thurlkill 10.921
EMBOSS 11.33
Sillero 10.935
Patrickios 11.199
IPC_peptide 11.125
IPC2_peptide 9.399
IPC2.peptide.svr19 8.309
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
23
0
23
3089
40
496
134.3
15.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.018 ± 0.402
1.036 ± 0.195
5.924 ± 0.455
8.514 ± 0.667
4.467 ± 0.259
5.277 ± 0.4
1.586 ± 0.26
7.219 ± 0.483
8.967 ± 0.549
9.938 ± 0.546
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.719 ± 0.289
5.18 ± 0.428
3.432 ± 0.47
3.852 ± 0.326
5.698 ± 0.459
5.601 ± 0.31
5.244 ± 0.516
5.083 ± 0.489
0.906 ± 0.216
4.338 ± 0.352
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here