Lactococcus insecticola
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1846 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6A0B7T7|A0A6A0B7T7_9LACT 2 5-diketo-D-gluconic acid reductase OS=Lactococcus insecticola OX=2709158 GN=Hs20B_08120 PE=4 SV=1
MM1 pKa = 7.16 NVNVYY6 pKa = 10.52 EE7 pKa = 4.31 MIKK10 pKa = 10.43 DD11 pKa = 3.5 DD12 pKa = 3.97 KK13 pKa = 10.94 FFIGSYY19 pKa = 9.35 PNNFAVGRR27 pKa = 11.84 WFTVEE32 pKa = 3.89 EE33 pKa = 4.25 LASKK37 pKa = 10.13 DD38 pKa = 2.88 WYY40 pKa = 10.33 EE41 pKa = 3.9 IEE43 pKa = 4.14 EE44 pKa = 4.55 EE45 pKa = 4.18 YY46 pKa = 11.07 LEE48 pKa = 4.77 KK49 pKa = 11.15 YY50 pKa = 10.66 NPDD53 pKa = 3.26 EE54 pKa = 4.32 YY55 pKa = 11.31 EE56 pKa = 3.95 EE57 pKa = 5.63 LEE59 pKa = 4.43 LGVFDD64 pKa = 4.93 VDD66 pKa = 4.04 NEE68 pKa = 4.4 SGLWRR73 pKa = 11.84 GEE75 pKa = 3.87 YY76 pKa = 10.14 DD77 pKa = 3.46 VSEE80 pKa = 5.43 LIDD83 pKa = 3.52 KK84 pKa = 10.47 LVEE87 pKa = 3.98 IFTTEE92 pKa = 4.07 YY93 pKa = 10.77 YY94 pKa = 10.72 DD95 pKa = 3.36 VDD97 pKa = 4.24 LEE99 pKa = 4.39 IFEE102 pKa = 4.31 FTQDD106 pKa = 3.57 FFDD109 pKa = 3.78 EE110 pKa = 4.7 MGFSAYY116 pKa = 9.39 EE117 pKa = 3.83 VAQMVFFGNIKK128 pKa = 9.89 SWGDD132 pKa = 3.31 EE133 pKa = 4.18 YY134 pKa = 10.95 IGFTGAGNFEE144 pKa = 5.63 SYY146 pKa = 8.91 TQSEE150 pKa = 5.0 YY151 pKa = 10.69 EE152 pKa = 3.93 AEE154 pKa = 3.82 ALEE157 pKa = 4.15 RR158 pKa = 11.84 VKK160 pKa = 11.12 DD161 pKa = 3.71 LGLFF165 pKa = 3.75
Molecular weight: 19.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.757
IPC_protein 3.706
Toseland 3.528
ProMoST 3.834
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.554
Rodwell 3.541
Grimsley 3.439
Solomon 3.656
Lehninger 3.605
Nozaki 3.783
DTASelect 3.923
Thurlkill 3.554
EMBOSS 3.567
Sillero 3.821
Patrickios 0.731
IPC_peptide 3.656
IPC2_peptide 3.795
IPC2.peptide.svr19 3.728
Protein with the highest isoelectric point:
>tr|A0A6A0B990|A0A6A0B990_9LACT Sodium:hydrogen antiporter OS=Lactococcus insecticola OX=2709158 GN=ydiF PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.29 QPSKK9 pKa = 10.26 RR10 pKa = 11.84 KK11 pKa = 8.87 RR12 pKa = 11.84 QKK14 pKa = 8.05 THH16 pKa = 5.1 GFRR19 pKa = 11.84 KK20 pKa = 10.02 RR21 pKa = 11.84 MSTKK25 pKa = 9.01 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VVASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.23 GRR39 pKa = 11.84 AVLSAA44 pKa = 3.96
Molecular weight: 5.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1846
0
1846
585584
42
6996
317.2
35.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.531 ± 0.083
0.308 ± 0.014
6.689 ± 0.161
6.345 ± 0.074
4.438 ± 0.069
6.371 ± 0.061
1.736 ± 0.031
7.21 ± 0.081
6.923 ± 0.106
9.068 ± 0.095
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.412 ± 0.039
4.785 ± 0.055
3.279 ± 0.042
3.655 ± 0.04
3.72 ± 0.065
6.675 ± 0.246
6.535 ± 0.108
7.053 ± 0.05
0.808 ± 0.021
3.461 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here