Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1)
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3835 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q2RYG6|Q2RYG6_RHORT Peptidase OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1) OX=269796 GN=Rru_A0024 PE=4 SV=1
MM1 pKa = 7.74 PYY3 pKa = 10.48 VVTEE7 pKa = 3.81 NCIKK11 pKa = 10.51 CKK13 pKa = 10.63 YY14 pKa = 7.8 QDD16 pKa = 3.86 CVEE19 pKa = 4.24 VCPVDD24 pKa = 3.79 CFYY27 pKa = 11.23 EE28 pKa = 4.55 GEE30 pKa = 4.2 NFLVINPDD38 pKa = 2.99 EE39 pKa = 5.52 CIDD42 pKa = 4.08 CGVCEE47 pKa = 4.44 PEE49 pKa = 4.91 CPAEE53 pKa = 4.84 AIFPDD58 pKa = 3.83 SEE60 pKa = 5.31 AIAGKK65 pKa = 9.0 WLEE68 pKa = 4.12 INRR71 pKa = 11.84 KK72 pKa = 8.47 FADD75 pKa = 3.78 LWPNITRR82 pKa = 11.84 KK83 pKa = 10.45 GPALADD89 pKa = 3.69 ADD91 pKa = 3.96 DD92 pKa = 4.53 WKK94 pKa = 11.19 DD95 pKa = 3.65 KK96 pKa = 10.37 PDD98 pKa = 3.43 KK99 pKa = 10.83 TGLLSEE105 pKa = 4.51 NPGKK109 pKa = 9.72 GTVV112 pKa = 3.13
Molecular weight: 12.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.929
IPC2_protein 4.228
IPC_protein 4.151
Toseland 3.961
ProMoST 4.279
Dawson 4.113
Bjellqvist 4.266
Wikipedia 4.012
Rodwell 3.973
Grimsley 3.872
Solomon 4.101
Lehninger 4.062
Nozaki 4.228
DTASelect 4.406
Thurlkill 3.986
EMBOSS 4.024
Sillero 4.253
Patrickios 1.952
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.164
Protein with the highest isoelectric point:
>tr|Q2RP35|Q2RP35_RHORT Phage integrase OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1) OX=269796 GN=Rru_A3316 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 8.96 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.73 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 LATVGGRR28 pKa = 11.84 RR29 pKa = 11.84 VLANRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.85 GRR39 pKa = 11.84 KK40 pKa = 8.87 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3835
0
3835
1292602
20
1981
337.1
36.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.276 ± 0.061
0.913 ± 0.013
5.797 ± 0.031
5.424 ± 0.035
3.403 ± 0.023
9.077 ± 0.041
1.969 ± 0.019
4.716 ± 0.027
2.789 ± 0.031
11.205 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.262 ± 0.019
2.048 ± 0.02
5.684 ± 0.036
2.823 ± 0.023
7.552 ± 0.041
5.084 ± 0.029
5.288 ± 0.031
7.499 ± 0.035
1.259 ± 0.015
1.934 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here