Eggerthella sp. (strain YY7918)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; Eggerthella; unclassified Eggerthella

Average proteome isoelectric point is 5.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2674 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F7UZP0|F7UZP0_EEGSY Lysine--tRNA ligase OS=Eggerthella sp. (strain YY7918) OX=502558 GN=LysU PE=3 SV=1
MM1 pKa = 7.57LLASLIALGVIAYY14 pKa = 8.41NYY16 pKa = 8.18WQGQQLYY23 pKa = 11.0DD24 pKa = 4.56EE25 pKa = 5.16IADD28 pKa = 3.64QGFVPPTDD36 pKa = 3.35IEE38 pKa = 4.44GTALADD44 pKa = 3.61ITVDD48 pKa = 2.9WDD50 pKa = 3.41ALKK53 pKa = 10.68AINPDD58 pKa = 3.42TVGWIYY64 pKa = 10.7IPDD67 pKa = 3.8TQVNYY72 pKa = 9.92PIVHH76 pKa = 5.23TTDD79 pKa = 2.79NEE81 pKa = 4.36KK82 pKa = 11.04YY83 pKa = 8.96LTRR86 pKa = 11.84DD87 pKa = 3.47FKK89 pKa = 11.22GTEE92 pKa = 3.6GWLAQYY98 pKa = 10.7GAIFLAAEE106 pKa = 4.14NKK108 pKa = 10.56GDD110 pKa = 4.32FSDD113 pKa = 4.76SNNILYY119 pKa = 8.9GHH121 pKa = 6.37NMQDD125 pKa = 2.89GSMFACVSGFTDD137 pKa = 3.47ATQFAEE143 pKa = 3.85HH144 pKa = 5.9RR145 pKa = 11.84TIYY148 pKa = 11.0LLTPQGNYY156 pKa = 10.04RR157 pKa = 11.84LQTFALIHH165 pKa = 5.17TTADD169 pKa = 3.41DD170 pKa = 5.6LIAQTTFTDD179 pKa = 3.74EE180 pKa = 4.2EE181 pKa = 3.85QRR183 pKa = 11.84RR184 pKa = 11.84AYY186 pKa = 10.46LQDD189 pKa = 4.1KK190 pKa = 9.68IDD192 pKa = 3.84RR193 pKa = 11.84SVASVDD199 pKa = 3.52DD200 pKa = 4.19VPAVSEE206 pKa = 4.24MSQSLMLSTCDD217 pKa = 4.13NLPTDD222 pKa = 3.63GRR224 pKa = 11.84YY225 pKa = 9.6VLYY228 pKa = 10.46AYY230 pKa = 10.22VAEE233 pKa = 4.48STVKK237 pKa = 10.6ASSDD241 pKa = 3.25ASQGGAASPDD251 pKa = 3.29AVDD254 pKa = 5.18AVDD257 pKa = 3.65EE258 pKa = 4.41ASKK261 pKa = 10.88EE262 pKa = 4.06LASS265 pKa = 3.79

Molecular weight: 29.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F7UZU6|F7UZU6_EEGSY Putative agmatine deiminase OS=Eggerthella sp. (strain YY7918) OX=502558 GN=aguA PE=3 SV=1
MM1 pKa = 7.06GAKK4 pKa = 9.37RR5 pKa = 11.84HH6 pKa = 4.96EE7 pKa = 4.08RR8 pKa = 11.84TRR10 pKa = 11.84ARR12 pKa = 11.84RR13 pKa = 11.84AAIQVLYY20 pKa = 9.69MGEE23 pKa = 3.84IRR25 pKa = 11.84EE26 pKa = 4.29LSPLTIAEE34 pKa = 4.42SDD36 pKa = 3.61DD37 pKa = 3.35SHH39 pKa = 8.56LIEE42 pKa = 5.24GGPIPVYY49 pKa = 10.62ALEE52 pKa = 4.24LVRR55 pKa = 11.84GVCEE59 pKa = 4.29HH60 pKa = 6.72PNEE63 pKa = 4.15VDD65 pKa = 3.27EE66 pKa = 5.81HH67 pKa = 5.65LTAPLKK73 pKa = 9.22TGRR76 pKa = 11.84WDD78 pKa = 3.57TLQNAWMARR87 pKa = 11.84HH88 pKa = 5.63GRR90 pKa = 11.84RR91 pKa = 11.84GRR93 pKa = 11.84CLRR96 pKa = 11.84SLRR99 pKa = 11.84YY100 pKa = 9.03FPRR103 pKa = 11.84AKK105 pKa = 10.46RR106 pKa = 11.84MLCAKK111 pKa = 9.94GMAWLFWRR119 pKa = 11.84LGAWRR124 pKa = 11.84RR125 pKa = 11.84ALGAAEE131 pKa = 4.33LLEE134 pKa = 4.33AQGVDD139 pKa = 3.02ARR141 pKa = 11.84VVDD144 pKa = 4.03MRR146 pKa = 11.84WVKK149 pKa = 10.31PLDD152 pKa = 3.75AEE154 pKa = 4.03EE155 pKa = 4.19MPRR158 pKa = 11.84VLLRR162 pKa = 11.84RR163 pKa = 11.84FEE165 pKa = 4.65STGSRR170 pKa = 11.84YY171 pKa = 10.23KK172 pKa = 10.44FWGRR176 pKa = 3.23

Molecular weight: 20.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2674

0

2674

912144

39

3501

341.1

37.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.195 ± 0.062

1.533 ± 0.024

5.972 ± 0.036

6.698 ± 0.047

3.952 ± 0.03

7.932 ± 0.047

1.837 ± 0.02

5.335 ± 0.037

3.951 ± 0.035

9.299 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.666 ± 0.023

3.115 ± 0.032

4.302 ± 0.031

2.989 ± 0.027

5.529 ± 0.067

5.922 ± 0.043

5.607 ± 0.05

8.109 ± 0.046

1.097 ± 0.016

2.96 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski