Amino acid dipepetide frequency for Clostridium sp. CAG:299

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.914AlaAla: 9.914 ± 0.163	1.278AlaCys: 1.278 ± 0.042	4.858AlaAsp: 4.858 ± 0.08	6.823AlaGlu: 6.823 ± 0.111	3.466AlaPhe: 3.466 ± 0.065	7.785AlaGly: 7.785 ± 0.106	1.129AlaHis: 1.129 ± 0.033	4.139AlaIle: 4.139 ± 0.092	4.235AlaLys: 4.235 ± 0.082	7.898AlaLeu: 7.898 ± 0.114	2.595AlaMet: 2.595 ± 0.058	2.056AlaAsn: 2.056 ± 0.057	2.33AlaPro: 2.33 ± 0.054	2.434AlaGln: 2.434 ± 0.06	3.754AlaArg: 3.754 ± 0.087	4.586AlaSer: 4.586 ± 0.078	2.406AlaThr: 2.406 ± 0.067	7.906AlaVal: 7.906 ± 0.117	0.688AlaTrp: 0.688 ± 0.03	2.623AlaTyr: 2.623 ± 0.063	0.012AlaXaa: 0.012 ± 0.004
Cys	1.089CysAla: 1.089 ± 0.043	0.351CysCys: 0.351 ± 0.024	0.726CysAsp: 0.726 ± 0.033	0.806CysGlu: 0.806 ± 0.034	0.668CysPhe: 0.668 ± 0.033	1.509CysGly: 1.509 ± 0.045	0.319CysHis: 0.319 ± 0.022	0.925CysIle: 0.925 ± 0.034	0.527CysLys: 0.527 ± 0.03	1.406CysLeu: 1.406 ± 0.044	0.462CysMet: 0.462 ± 0.025	0.417CysAsn: 0.417 ± 0.024	0.672CysPro: 0.672 ± 0.034	0.529CysGln: 0.529 ± 0.026	1.123CysArg: 1.123 ± 0.044	1.091CysSer: 1.091 ± 0.038	0.685CysThr: 0.685 ± 0.03	1.007CysVal: 1.007 ± 0.036	0.124CysTrp: 0.124 ± 0.013	0.502CysTyr: 0.502 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	3.905AspAla: 3.905 ± 0.08	0.82AspCys: 0.82 ± 0.033	2.402AspAsp: 2.402 ± 0.075	4.212AspGlu: 4.212 ± 0.079	2.504AspPhe: 2.504 ± 0.063	4.596AspGly: 4.596 ± 0.099	0.874AspHis: 0.874 ± 0.04	3.675AspIle: 3.675 ± 0.07	2.785AspLys: 2.785 ± 0.068	4.63AspLeu: 4.63 ± 0.086	1.832AspMet: 1.832 ± 0.055	1.645AspAsn: 1.645 ± 0.048	1.936AspPro: 1.936 ± 0.052	1.566AspGln: 1.566 ± 0.043	2.997AspArg: 2.997 ± 0.072	3.16AspSer: 3.16 ± 0.062	2.671AspThr: 2.671 ± 0.057	3.34AspVal: 3.34 ± 0.073	0.617AspTrp: 0.617 ± 0.028	2.394AspTyr: 2.394 ± 0.06	0.001AspXaa: 0.001 ± 0.001
Glu	6.845GluAla: 6.845 ± 0.115	0.746GluCys: 0.746 ± 0.037	4.289GluAsp: 4.289 ± 0.08	9.025GluGlu: 9.025 ± 0.167	2.679GluPhe: 2.679 ± 0.055	5.219GluGly: 5.219 ± 0.088	1.347GluHis: 1.347 ± 0.045	5.693GluIle: 5.693 ± 0.098	6.866GluLys: 6.866 ± 0.129	7.503GluLeu: 7.503 ± 0.102	2.514GluMet: 2.514 ± 0.061	3.731GluAsn: 3.731 ± 0.073	2.386GluPro: 2.386 ± 0.059	3.367GluGln: 3.367 ± 0.072	4.358GluArg: 4.358 ± 0.099	3.609GluSer: 3.609 ± 0.084	4.618GluThr: 4.618 ± 0.101	3.819GluVal: 3.819 ± 0.08	0.625GluTrp: 0.625 ± 0.028	2.885GluTyr: 2.885 ± 0.066	0.003GluXaa: 0.003 ± 0.002
Phe	3.105PheAla: 3.105 ± 0.076	0.812PheCys: 0.812 ± 0.033	2.355PheAsp: 2.355 ± 0.052	2.667PheGlu: 2.667 ± 0.069	1.958PhePhe: 1.958 ± 0.06	3.348PheGly: 3.348 ± 0.073	0.893PheHis: 0.893 ± 0.036	2.586PheIle: 2.586 ± 0.076	1.858PheLys: 1.858 ± 0.048	4.544PheLeu: 4.544 ± 0.093	1.181PheMet: 1.181 ± 0.039	1.318PheAsn: 1.318 ± 0.039	1.577PhePro: 1.577 ± 0.042	1.451PheGln: 1.451 ± 0.045	1.865PheArg: 1.865 ± 0.059	2.99PheSer: 2.99 ± 0.055	2.306PheThr: 2.306 ± 0.055	2.637PheVal: 2.637 ± 0.063	0.388PheTrp: 0.388 ± 0.023	1.627PheTyr: 1.627 ± 0.051	0.003PheXaa: 0.003 ± 0.002
Gly	5.897GlyAla: 5.897 ± 0.115	1.383GlyCys: 1.383 ± 0.044	3.555GlyAsp: 3.555 ± 0.071	5.651GlyGlu: 5.651 ± 0.096	3.378GlyPhe: 3.378 ± 0.066	5.623GlyGly: 5.623 ± 0.124	1.247GlyHis: 1.247 ± 0.04	6.232GlyIle: 6.232 ± 0.107	5.305GlyLys: 5.305 ± 0.091	6.668GlyLeu: 6.668 ± 0.115	2.615GlyMet: 2.615 ± 0.071	2.841GlyAsn: 2.841 ± 0.058	1.701GlyPro: 1.701 ± 0.053	2.589GlyGln: 2.589 ± 0.063	4.199GlyArg: 4.199 ± 0.089	4.717GlySer: 4.717 ± 0.082	4.642GlyThr: 4.642 ± 0.082	5.175GlyVal: 5.175 ± 0.095	0.888GlyTrp: 0.888 ± 0.036	3.049GlyTyr: 3.049 ± 0.066	0.013GlyXaa: 0.013 ± 0.004
His	1.049HisAla: 1.049 ± 0.036	0.274HisCys: 0.274 ± 0.016	0.841HisAsp: 0.841 ± 0.038	1.011HisGlu: 1.011 ± 0.04	0.812HisPhe: 0.812 ± 0.033	1.287HisGly: 1.287 ± 0.048	0.356HisHis: 0.356 ± 0.026	1.263HisIle: 1.263 ± 0.043	0.804HisLys: 0.804 ± 0.035	1.555HisLeu: 1.555 ± 0.048	0.594HisMet: 0.594 ± 0.023	0.603HisAsn: 0.603 ± 0.033	0.927HisPro: 0.927 ± 0.035	0.563HisGln: 0.563 ± 0.028	0.853HisArg: 0.853 ± 0.036	1.093HisSer: 1.093 ± 0.044	0.865HisThr: 0.865 ± 0.03	1.136HisVal: 1.136 ± 0.042	0.171HisTrp: 0.171 ± 0.016	0.668HisTyr: 0.668 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.335IleAla: 5.335 ± 0.101	1.263IleCys: 1.263 ± 0.044	3.455IleAsp: 3.455 ± 0.075	4.612IleGlu: 4.612 ± 0.074	2.643IlePhe: 2.643 ± 0.072	5.012IleGly: 5.012 ± 0.096	1.306IleHis: 1.306 ± 0.036	3.985IleIle: 3.985 ± 0.079	3.303IleLys: 3.303 ± 0.071	6.708IleLeu: 6.708 ± 0.112	1.689IleMet: 1.689 ± 0.052	2.224IleAsn: 2.224 ± 0.056	3.144IlePro: 3.144 ± 0.068	2.276IleGln: 2.276 ± 0.057	3.745IleArg: 3.745 ± 0.076	4.325IleSer: 4.325 ± 0.077	3.487IleThr: 3.487 ± 0.078	4.384IleVal: 4.384 ± 0.082	0.625IleTrp: 0.625 ± 0.023	2.267IleTyr: 2.267 ± 0.056	0.003IleXaa: 0.003 ± 0.002
Lys	5.233LysAla: 5.233 ± 0.102	0.571LysCys: 0.571 ± 0.029	3.235LysAsp: 3.235 ± 0.064	6.416LysGlu: 6.416 ± 0.123	1.583LysPhe: 1.583 ± 0.047	4.165LysGly: 4.165 ± 0.065	0.902LysHis: 0.902 ± 0.037	3.96LysIle: 3.96 ± 0.08	5.547LysLys: 5.547 ± 0.105	4.905LysLeu: 4.905 ± 0.088	1.924LysMet: 1.924 ± 0.058	2.934LysAsn: 2.934 ± 0.059	1.985LysPro: 1.985 ± 0.048	2.203LysGln: 2.203 ± 0.056	3.523LysArg: 3.523 ± 0.071	2.962LysSer: 2.962 ± 0.067	3.675LysThr: 3.675 ± 0.069	3.328LysVal: 3.328 ± 0.08	0.486LysTrp: 0.486 ± 0.025	2.077LysTyr: 2.077 ± 0.058	0.001LysXaa: 0.001 ± 0.001
Leu	8.019LeuAla: 8.019 ± 0.118	1.598LeuCys: 1.598 ± 0.047	5.077LeuAsp: 5.077 ± 0.085	6.793LeuGlu: 6.793 ± 0.1	4.113LeuPhe: 4.113 ± 0.088	6.491LeuGly: 6.491 ± 0.119	1.574LeuHis: 1.574 ± 0.048	5.845LeuIle: 5.845 ± 0.102	6.212LeuLys: 6.212 ± 0.101	9.356LeuLeu: 9.356 ± 0.162	2.781LeuMet: 2.781 ± 0.067	3.681LeuAsn: 3.681 ± 0.074	3.905LeuPro: 3.905 ± 0.073	2.585LeuGln: 2.585 ± 0.059	4.439LeuArg: 4.439 ± 0.086	6.833LeuSer: 6.833 ± 0.099	5.141LeuThr: 5.141 ± 0.085	5.2LeuVal: 5.2 ± 0.082	0.756LeuTrp: 0.756 ± 0.03	3.188LeuTyr: 3.188 ± 0.069	0.005LeuXaa: 0.005 ± 0.003
Met	3.017MetAla: 3.017 ± 0.071	0.338MetCys: 0.338 ± 0.02	1.848MetAsp: 1.848 ± 0.052	3.032MetGlu: 3.032 ± 0.07	1.041MetPhe: 1.041 ± 0.04	2.336MetGly: 2.336 ± 0.057	0.372MetHis: 0.372 ± 0.023	2.115MetIle: 2.115 ± 0.054	2.57MetLys: 2.57 ± 0.055	2.439MetLeu: 2.439 ± 0.056	1.013MetMet: 1.013 ± 0.04	1.454MetAsn: 1.454 ± 0.046	1.131MetPro: 1.131 ± 0.04	0.858MetGln: 0.858 ± 0.037	1.371MetArg: 1.371 ± 0.041	1.583MetSer: 1.583 ± 0.049	1.868MetThr: 1.868 ± 0.046	1.958MetVal: 1.958 ± 0.052	0.194MetTrp: 0.194 ± 0.015	0.844MetTyr: 0.844 ± 0.03	0.003MetXaa: 0.003 ± 0.002
Asn	2.713AsnAla: 2.713 ± 0.063	0.526AsnCys: 0.526 ± 0.026	1.606AsnAsp: 1.606 ± 0.047	2.371AsnGlu: 2.371 ± 0.058	1.371AsnPhe: 1.371 ± 0.047	3.147AsnGly: 3.147 ± 0.069	0.712AsnHis: 0.712 ± 0.035	2.559AsnIle: 2.559 ± 0.065	1.812AsnLys: 1.812 ± 0.052	3.392AsnLeu: 3.392 ± 0.078	1.228AsnMet: 1.228 ± 0.039	1.185AsnAsn: 1.185 ± 0.039	1.937AsnPro: 1.937 ± 0.052	1.475AsnGln: 1.475 ± 0.047	2.185AsnArg: 2.185 ± 0.056	1.988AsnSer: 1.988 ± 0.06	1.77AsnThr: 1.77 ± 0.047	2.342AsnVal: 2.342 ± 0.064	0.342AsnTrp: 0.342 ± 0.021	1.424AsnTyr: 1.424 ± 0.049	0.0AsnXaa: 0.0 ± 0.0
Pro	3.088ProAla: 3.088 ± 0.073	0.453ProCys: 0.453 ± 0.028	2.387ProAsp: 2.387 ± 0.054	4.041ProGlu: 4.041 ± 0.078	1.72ProPhe: 1.72 ± 0.052	3.057ProGly: 3.057 ± 0.061	0.606ProHis: 0.606 ± 0.027	1.862ProIle: 1.862 ± 0.057	1.758ProLys: 1.758 ± 0.058	3.14ProLeu: 3.14 ± 0.06	1.004ProMet: 1.004 ± 0.038	1.065ProAsn: 1.065 ± 0.038	0.873ProPro: 0.873 ± 0.038	1.029ProGln: 1.029 ± 0.035	1.169ProArg: 1.169 ± 0.042	2.115ProSer: 2.115 ± 0.059	1.236ProThr: 1.236 ± 0.043	3.241ProVal: 3.241 ± 0.061	0.378ProTrp: 0.378 ± 0.023	1.352ProTyr: 1.352 ± 0.042	0.004ProXaa: 0.004 ± 0.002
Gln	3.14GlnAla: 3.14 ± 0.072	0.346GlnCys: 0.346 ± 0.022	1.539GlnAsp: 1.539 ± 0.042	2.945GlnGlu: 2.945 ± 0.073	1.299GlnPhe: 1.299 ± 0.038	2.204GlnGly: 2.204 ± 0.056	0.371GlnHis: 0.371 ± 0.021	2.581GlnIle: 2.581 ± 0.053	2.4GlnLys: 2.4 ± 0.061	2.844GlnLeu: 2.844 ± 0.068	1.363GlnMet: 1.363 ± 0.037	1.434GlnAsn: 1.434 ± 0.049	1.013GlnPro: 1.013 ± 0.039	0.864GlnGln: 0.864 ± 0.035	1.399GlnArg: 1.399 ± 0.043	1.618GlnSer: 1.618 ± 0.047	1.894GlnThr: 1.894 ± 0.052	2.403GlnVal: 2.403 ± 0.063	0.36GlnTrp: 0.36 ± 0.021	1.247GlnTyr: 1.247 ± 0.038	0.003GlnXaa: 0.003 ± 0.002
Arg	3.451ArgAla: 3.451 ± 0.079	0.625ArgCys: 0.625 ± 0.031	2.513ArgAsp: 2.513 ± 0.059	5.426ArgGlu: 5.426 ± 0.112	2.151ArgPhe: 2.151 ± 0.061	3.137ArgGly: 3.137 ± 0.068	0.84ArgHis: 0.84 ± 0.031	3.639ArgIle: 3.639 ± 0.084	3.583ArgLys: 3.583 ± 0.071	5.223ArgLeu: 5.223 ± 0.105	1.738ArgMet: 1.738 ± 0.049	1.87ArgAsn: 1.87 ± 0.057	1.736ArgPro: 1.736 ± 0.045	2.278ArgGln: 2.278 ± 0.068	3.304ArgArg: 3.304 ± 0.088	2.456ArgSer: 2.456 ± 0.059	2.647ArgThr: 2.647 ± 0.057	2.96ArgVal: 2.96 ± 0.069	0.441ArgTrp: 0.441 ± 0.026	1.888ArgTyr: 1.888 ± 0.052	0.0ArgXaa: 0.0 ± 0.0
Ser	4.671SerAla: 4.671 ± 0.081	0.968SerCys: 0.968 ± 0.037	2.936SerAsp: 2.936 ± 0.063	4.107SerGlu: 4.107 ± 0.076	2.864SerPhe: 2.864 ± 0.063	5.706SerGly: 5.706 ± 0.106	1.079SerHis: 1.079 ± 0.039	3.649SerIle: 3.649 ± 0.078	2.535SerLys: 2.535 ± 0.06	5.675SerLeu: 5.675 ± 0.086	1.858SerMet: 1.858 ± 0.049	1.665SerAsn: 1.665 ± 0.06	1.97SerPro: 1.97 ± 0.061	2.181SerGln: 2.181 ± 0.055	3.581SerArg: 3.581 ± 0.083	3.906SerSer: 3.906 ± 0.085	2.315SerThr: 2.315 ± 0.056	4.444SerVal: 4.444 ± 0.076	0.618SerTrp: 0.618 ± 0.029	2.283SerTyr: 2.283 ± 0.056	0.001SerXaa: 0.001 ± 0.001
Thr	5.195ThrAla: 5.195 ± 0.081	0.701ThrCys: 0.701 ± 0.032	2.877ThrAsp: 2.877 ± 0.059	4.125ThrGlu: 4.125 ± 0.093	1.953ThrPhe: 1.953 ± 0.053	4.953ThrGly: 4.953 ± 0.095	0.774ThrHis: 0.774 ± 0.031	3.227ThrIle: 3.227 ± 0.077	2.602ThrLys: 2.602 ± 0.059	4.424ThrLeu: 4.424 ± 0.076	1.448ThrMet: 1.448 ± 0.04	1.506ThrAsn: 1.506 ± 0.047	2.099ThrPro: 2.099 ± 0.057	1.247ThrGln: 1.247 ± 0.041	2.049ThrArg: 2.049 ± 0.048	2.574ThrSer: 2.574 ± 0.064	2.221ThrThr: 2.221 ± 0.071	4.583ThrVal: 4.583 ± 0.066	0.457ThrTrp: 0.457 ± 0.024	1.643ThrTyr: 1.643 ± 0.052	0.0ThrXaa: 0.0 ± 0.0
Val	4.204ValAla: 4.204 ± 0.081	1.214ValCys: 1.214 ± 0.044	3.43ValAsp: 3.43 ± 0.076	4.468ValGlu: 4.468 ± 0.088	3.18ValPhe: 3.18 ± 0.064	4.244ValGly: 4.244 ± 0.095	1.119ValHis: 1.119 ± 0.041	4.969ValIle: 4.969 ± 0.082	4.482ValLys: 4.482 ± 0.085	6.782ValLeu: 6.782 ± 0.101	2.173ValMet: 2.173 ± 0.062	2.661ValAsn: 2.661 ± 0.072	2.639ValPro: 2.639 ± 0.062	1.941ValGln: 1.941 ± 0.049	3.33ValArg: 3.33 ± 0.077	4.834ValSer: 4.834 ± 0.083	3.841ValThr: 3.841 ± 0.081	4.384ValVal: 4.384 ± 0.105	0.617ValTrp: 0.617 ± 0.03	2.57ValTyr: 2.57 ± 0.056	0.009ValXaa: 0.009 ± 0.003
Trp	0.589TrpAla: 0.589 ± 0.029	0.125TrpCys: 0.125 ± 0.014	0.541TrpAsp: 0.541 ± 0.027	0.713TrpGlu: 0.713 ± 0.031	0.445TrpPhe: 0.445 ± 0.023	0.67TrpGly: 0.67 ± 0.034	0.159TrpHis: 0.159 ± 0.016	0.673TrpIle: 0.673 ± 0.034	0.692TrpLys: 0.692 ± 0.031	0.905TrpLeu: 0.905 ± 0.035	0.362TrpMet: 0.362 ± 0.021	0.459TrpAsn: 0.459 ± 0.026	0.259TrpPro: 0.259 ± 0.018	0.332TrpGln: 0.332 ± 0.022	0.405TrpArg: 0.405 ± 0.022	0.421TrpSer: 0.421 ± 0.028	0.433TrpThr: 0.433 ± 0.026	0.45TrpVal: 0.45 ± 0.024	0.119TrpTrp: 0.119 ± 0.012	0.439TrpTyr: 0.439 ± 0.027	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.44TyrAla: 2.44 ± 0.058	0.543TyrCys: 0.543 ± 0.028	2.093TyrAsp: 2.093 ± 0.058	2.876TyrGlu: 2.876 ± 0.065	1.645TyrPhe: 1.645 ± 0.051	2.87TyrGly: 2.87 ± 0.062	0.744TyrHis: 0.744 ± 0.03	2.215TyrIle: 2.215 ± 0.057	1.741TyrLys: 1.741 ± 0.056	3.589TyrLeu: 3.589 ± 0.073	1.008TyrMet: 1.008 ± 0.031	1.334TyrAsn: 1.334 ± 0.047	1.358TyrPro: 1.358 ± 0.046	1.495TyrGln: 1.495 ± 0.047	2.246TyrArg: 2.246 ± 0.059	2.149TyrSer: 2.149 ± 0.053	1.937TyrThr: 1.937 ± 0.054	2.391TyrVal: 2.391 ± 0.06	0.299TyrTrp: 0.299 ± 0.021	1.609TyrTyr: 1.609 ± 0.046	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.013XaaAla: 0.013 ± 0.004	0.003XaaCys: 0.003 ± 0.002	0.0XaaAsp: 0.0 ± 0.0	0.005XaaGlu: 0.005 ± 0.003	0.0XaaPhe: 0.0 ± 0.0	0.008XaaGly: 0.008 ± 0.003	0.001XaaHis: 0.001 ± 0.001	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.002	0.004XaaLeu: 0.004 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.003XaaAsn: 0.003 ± 0.002	0.004XaaPro: 0.004 ± 0.002	0.004XaaGln: 0.004 ± 0.002	0.011XaaArg: 0.011 ± 0.004	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.003XaaTyr: 0.003 ± 0.002	0.081XaaXaa: 0.081 ± 0.013

Statistics based on 2441 proteins (749053 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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