Torque teno mini virus ALH8
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A076V5S0|A0A076V5S0_9VIRU Uncharacterized protein OS=Torque teno mini virus ALH8 OX=1535291 GN=ALH8_gp1 PE=4 SV=1
MM1 pKa = 7.51 HH2 pKa = 7.85 KK3 pKa = 9.62 LTRR6 pKa = 11.84 AQQIKK11 pKa = 8.2 FTNCTVGIHH20 pKa = 7.41 DD21 pKa = 5.23 CLCDD25 pKa = 3.72 CDD27 pKa = 5.68 KK28 pKa = 10.84 PALHH32 pKa = 7.11 AATILLKK39 pKa = 10.66 QLGPEE44 pKa = 4.39 LLQEE48 pKa = 4.61 EE49 pKa = 4.67 KK50 pKa = 11.09 DD51 pKa = 3.47 HH52 pKa = 7.37 LKK54 pKa = 10.6 RR55 pKa = 11.84 CLGTTTGPVEE65 pKa = 4.28 EE66 pKa = 4.85 DD67 pKa = 3.43 CGVTGEE73 pKa = 4.51 DD74 pKa = 3.85 LEE76 pKa = 4.59 KK77 pKa = 11.09 LFAEE81 pKa = 4.83 DD82 pKa = 3.41 TGEE85 pKa = 4.34 DD86 pKa = 3.32 QDD88 pKa = 4.06 GG89 pKa = 3.52
Molecular weight: 9.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.593
IPC2_protein 4.673
IPC_protein 4.558
Toseland 4.418
ProMoST 4.685
Dawson 4.52
Bjellqvist 4.673
Wikipedia 4.406
Rodwell 4.418
Grimsley 4.329
Solomon 4.52
Lehninger 4.482
Nozaki 4.635
DTASelect 4.813
Thurlkill 4.431
EMBOSS 4.431
Sillero 4.685
Patrickios 3.63
IPC_peptide 4.52
IPC2_peptide 4.685
IPC2.peptide.svr19 4.613
Protein with the highest isoelectric point:
>tr|A0A076V5S0|A0A076V5S0_9VIRU Uncharacterized protein OS=Torque teno mini virus ALH8 OX=1535291 GN=ALH8_gp1 PE=4 SV=1
MM1 pKa = 7.58 PWYY4 pKa = 10.17 NYY6 pKa = 7.45 WPRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 LRR14 pKa = 11.84 RR15 pKa = 11.84 YY16 pKa = 6.68 WRR18 pKa = 11.84 RR19 pKa = 11.84 PRR21 pKa = 11.84 KK22 pKa = 6.91 TLRR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 YY28 pKa = 7.5 WRR30 pKa = 11.84 RR31 pKa = 11.84 PRR33 pKa = 11.84 WVRR36 pKa = 11.84 RR37 pKa = 11.84 PYY39 pKa = 10.14 KK40 pKa = 10.1 RR41 pKa = 11.84 KK42 pKa = 9.28 LRR44 pKa = 11.84 KK45 pKa = 8.97 IVLTQFQPKK54 pKa = 8.98 TIRR57 pKa = 11.84 KK58 pKa = 8.78 SKK60 pKa = 10.38 IKK62 pKa = 10.81 GPICLFQTTNKK73 pKa = 10.22 RR74 pKa = 11.84 LVNNFDD80 pKa = 3.38 MYY82 pKa = 9.89 EE83 pKa = 3.94 TSEE86 pKa = 4.36 VPEE89 pKa = 4.13 HH90 pKa = 5.94 QPGGGGWGIKK100 pKa = 10.13 VFTLEE105 pKa = 4.03 GLYY108 pKa = 10.29 SEE110 pKa = 5.82 HH111 pKa = 7.54 IYY113 pKa = 10.75 GRR115 pKa = 11.84 NIWTVTNQDD124 pKa = 3.73 LPLVRR129 pKa = 11.84 YY130 pKa = 8.33 LGCSIRR136 pKa = 11.84 FYY138 pKa = 10.53 QSEE141 pKa = 4.35 YY142 pKa = 7.9 TDD144 pKa = 3.79 YY145 pKa = 11.36 VATYY149 pKa = 10.69 SNQLPLQSSLGMYY162 pKa = 10.59 NAMQPNIHH170 pKa = 6.84 LLLKK174 pKa = 10.83 NKK176 pKa = 8.46 ITMPGLKK183 pKa = 8.92 TYY185 pKa = 9.67 KK186 pKa = 10.25 RR187 pKa = 11.84 KK188 pKa = 10.12 VPYY191 pKa = 9.83 KK192 pKa = 10.09 KK193 pKa = 10.7 VFVPPPTQLEE203 pKa = 4.38 NKK205 pKa = 9.11 WYY207 pKa = 9.57 FQQNMAKK214 pKa = 9.01 TPLLMTRR221 pKa = 11.84 VTACSLNKK229 pKa = 10.04 FYY231 pKa = 10.57 IDD233 pKa = 3.79 PDD235 pKa = 4.3 HH236 pKa = 7.23 INTNLTITSLNVSLFSNRR254 pKa = 11.84 QFQDD258 pKa = 2.76 ATDD261 pKa = 3.67 YY262 pKa = 11.13 HH263 pKa = 6.95 PKK265 pKa = 9.3 TVGTINYY272 pKa = 8.65 YY273 pKa = 10.6 LYY275 pKa = 9.9 STTQNPPYY283 pKa = 10.36 NGPLKK288 pKa = 10.77 LKK290 pKa = 9.85 MLIPLTDD297 pKa = 3.22 TMIYY301 pKa = 10.18 KK302 pKa = 10.4 PGINYY307 pKa = 9.92 EE308 pKa = 3.97 EE309 pKa = 4.38 YY310 pKa = 10.59 KK311 pKa = 10.86 RR312 pKa = 11.84 NNTTKK317 pKa = 10.27 SWTDD321 pKa = 2.52 WKK323 pKa = 9.94 TEE325 pKa = 3.7 YY326 pKa = 10.22 TIYY329 pKa = 10.69 AGNPFHH335 pKa = 6.87 TNYY338 pKa = 10.39 LSVGEE343 pKa = 4.03 PVFLIGKK350 pKa = 9.15 KK351 pKa = 10.15 PSDD354 pKa = 3.52 LFSSEE359 pKa = 4.15 EE360 pKa = 4.55 GEE362 pKa = 4.25 TSDD365 pKa = 3.57 YY366 pKa = 7.91 TTAEE370 pKa = 3.83 LTKK373 pKa = 9.73 TIRR376 pKa = 11.84 YY377 pKa = 8.66 NPYY380 pKa = 9.99 NDD382 pKa = 3.44 QGQTNICYY390 pKa = 10.23 FKK392 pKa = 11.35 ANFKK396 pKa = 11.0 SEE398 pKa = 4.4 TSWQPPDD405 pKa = 4.59 NPDD408 pKa = 3.26 LTNEE412 pKa = 4.21 NLPFWLLLWGFSDD425 pKa = 3.17 WHH427 pKa = 6.21 KK428 pKa = 10.71 KK429 pKa = 8.36 IKK431 pKa = 9.96 KK432 pKa = 8.83 HH433 pKa = 5.17 LHH435 pKa = 5.83 IDD437 pKa = 3.07 SSYY440 pKa = 9.51 ILVMRR445 pKa = 11.84 HH446 pKa = 5.73 IPLALNTEE454 pKa = 4.63 YY455 pKa = 10.25 IVPLSDD461 pKa = 4.88 SFLQGKK467 pKa = 9.31 SPFSPEE473 pKa = 3.92 EE474 pKa = 3.71 QPIGADD480 pKa = 2.84 RR481 pKa = 11.84 TTWHH485 pKa = 6.84 PQFQHH490 pKa = 4.83 QAEE493 pKa = 4.96 AINTICSAGPGTTKK507 pKa = 10.32 IPDD510 pKa = 3.59 NYY512 pKa = 10.6 SVQGLMKK519 pKa = 10.45 YY520 pKa = 10.3 SFYY523 pKa = 10.72 FKK525 pKa = 9.98 WGGSPPPMSTIEE537 pKa = 5.36 DD538 pKa = 5.32 PIQQPTYY545 pKa = 9.7 PVPHH549 pKa = 6.77 NFNLTTSLQNPEE561 pKa = 4.17 TDD563 pKa = 3.31 PASILWSFDD572 pKa = 3.14 QRR574 pKa = 11.84 RR575 pKa = 11.84 HH576 pKa = 5.33 SLTKK580 pKa = 10.17 KK581 pKa = 10.43 AIEE584 pKa = 4.19 RR585 pKa = 11.84 LQKK588 pKa = 9.94 DD589 pKa = 3.48 TGIKK593 pKa = 9.41 TITVTGGSHH602 pKa = 7.55 FDD604 pKa = 3.54 PAPTISQEE612 pKa = 4.17 EE613 pKa = 4.26 TSEE616 pKa = 4.06 EE617 pKa = 3.98 SSEE620 pKa = 4.18 EE621 pKa = 3.95 EE622 pKa = 4.39 KK623 pKa = 10.17 EE624 pKa = 4.28 TSLLEE629 pKa = 4.58 KK630 pKa = 10.39 LQQQQRR636 pKa = 11.84 QQRR639 pKa = 11.84 RR640 pKa = 11.84 LKK642 pKa = 10.36 LRR644 pKa = 11.84 IMKK647 pKa = 8.95 TLLKK651 pKa = 10.08 IQQLEE656 pKa = 4.0
Molecular weight: 77.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.826
IPC2_protein 9.107
IPC_protein 9.004
Toseland 9.531
ProMoST 9.37
Dawson 9.853
Bjellqvist 9.575
Wikipedia 10.058
Rodwell 10.087
Grimsley 9.94
Solomon 9.867
Lehninger 9.809
Nozaki 9.575
DTASelect 9.56
Thurlkill 9.663
EMBOSS 9.97
Sillero 9.765
Patrickios 4.673
IPC_peptide 9.853
IPC2_peptide 8.17
IPC2.peptide.svr19 7.99
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
745
89
656
372.5
43.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.819 ± 1.317
1.477 ± 2.477
4.43 ± 2.673
5.772 ± 2.042
3.624 ± 0.648
5.101 ± 1.829
2.55 ± 0.914
5.638 ± 1.066
7.517 ± 0.164
9.53 ± 1.859
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.879 ± 0.355
5.235 ± 1.934
7.383 ± 1.887
5.772 ± 0.072
5.369 ± 1.469
5.772 ± 2.715
9.396 ± 0.337
3.221 ± 0.07
2.282 ± 1.073
5.235 ± 2.463
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here