Lactobacillus phage iA2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Iaduovirus; Lactobacillus virus iA2

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0IXM5|A0A0P0IXM5_9CAUD Holliday junction resolvase OS=Lactobacillus phage iA2 OX=1739609 GN=iA2_46 PE=4 SV=1
MM1 pKa = 7.8SLHH4 pKa = 5.72QWDD7 pKa = 5.0NINDD11 pKa = 3.51LHH13 pKa = 7.2HH14 pKa = 6.98AEE16 pKa = 3.88KK17 pKa = 10.79SGWGEE22 pKa = 3.48EE23 pKa = 4.46SKK25 pKa = 11.17FEE27 pKa = 4.47EE28 pKa = 5.07LEE30 pKa = 4.37DD31 pKa = 3.8YY32 pKa = 10.6QGNALHH38 pKa = 7.04PGHH41 pKa = 6.74TYY43 pKa = 9.7WLYY46 pKa = 10.24QGEE49 pKa = 4.59LFDD52 pKa = 4.74EE53 pKa = 4.95DD54 pKa = 3.77EE55 pKa = 4.63ALEE58 pKa = 4.18FLDD61 pKa = 5.34SLGATQVINN70 pKa = 3.66

Molecular weight: 8.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0IDB8|A0A0P0IDB8_9CAUD Uncharacterized protein OS=Lactobacillus phage iA2 OX=1739609 GN=iA2_9 PE=4 SV=1
MM1 pKa = 7.79SRR3 pKa = 11.84LLDD6 pKa = 4.19DD7 pKa = 4.83KK8 pKa = 10.89QLKK11 pKa = 9.8AYY13 pKa = 9.68KK14 pKa = 10.06KK15 pKa = 9.55FVPGHH20 pKa = 5.82TGAEE24 pKa = 4.03IAKK27 pKa = 8.66MVYY30 pKa = 9.72EE31 pKa = 3.84NWGIQLTVQKK41 pKa = 10.43VHH43 pKa = 6.79ALNIRR48 pKa = 11.84NNIKK52 pKa = 10.27SGLYY56 pKa = 9.24QKK58 pKa = 10.97YY59 pKa = 9.6FGKK62 pKa = 10.45ADD64 pKa = 3.54PRR66 pKa = 11.84RR67 pKa = 11.84SSSHH71 pKa = 6.52HH72 pKa = 6.72DD73 pKa = 3.1LHH75 pKa = 8.37KK76 pKa = 10.87RR77 pKa = 11.84MAIGTVKK84 pKa = 10.57RR85 pKa = 11.84NEE87 pKa = 4.08TRR89 pKa = 11.84SKK91 pKa = 10.78DD92 pKa = 3.59RR93 pKa = 11.84PNRR96 pKa = 11.84APIVVVKK103 pKa = 10.04QAEE106 pKa = 4.5RR107 pKa = 11.84KK108 pKa = 7.34WKK110 pKa = 8.74PNHH113 pKa = 5.12RR114 pKa = 11.84RR115 pKa = 11.84VWEE118 pKa = 3.92EE119 pKa = 3.75AYY121 pKa = 10.54GPIPKK126 pKa = 10.1GYY128 pKa = 8.45KK129 pKa = 8.98TVFLDD134 pKa = 4.03GNSLNFSITNLALVTDD150 pKa = 4.43AEE152 pKa = 4.32FLVMNDD158 pKa = 2.74KK159 pKa = 10.96HH160 pKa = 7.16LISSDD165 pKa = 3.03KK166 pKa = 10.55RR167 pKa = 11.84VTRR170 pKa = 11.84SGIALARR177 pKa = 11.84LLSKK181 pKa = 9.5TYY183 pKa = 8.55QVKK186 pKa = 10.24RR187 pKa = 11.84KK188 pKa = 9.76KK189 pKa = 10.76GSNN192 pKa = 3.16

Molecular weight: 22.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

10725

30

1468

214.5

23.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.569 ± 0.58

0.326 ± 0.104

6.844 ± 0.374

5.781 ± 0.46

3.431 ± 0.175

6.406 ± 0.355

1.893 ± 0.176

6.145 ± 0.241

7.096 ± 0.458

8.214 ± 0.335

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.527 ± 0.229

5.604 ± 0.368

3.226 ± 0.233

4.606 ± 0.182

4.438 ± 0.396

6.788 ± 0.356

6.62 ± 0.528

6.499 ± 0.23

1.557 ± 0.156

3.431 ± 0.295
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski