Amino acid dipepetide frequency for Paenibacillus phage Likha

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.779AlaAla: 4.779 ± 0.961
0.558AlaCys: 0.558 ± 0.17
3.982AlaAsp: 3.982 ± 0.543
6.292AlaGlu: 6.292 ± 0.739
1.752AlaPhe: 1.752 ± 0.365
4.619AlaGly: 4.619 ± 0.746
0.717AlaHis: 0.717 ± 0.212
4.699AlaIle: 4.699 ± 0.756
5.814AlaLys: 5.814 ± 0.744
5.097AlaLeu: 5.097 ± 0.643
1.195AlaMet: 1.195 ± 0.297
3.106AlaAsn: 3.106 ± 0.487
1.752AlaPro: 1.752 ± 0.33
2.15AlaGln: 2.15 ± 0.4
2.31AlaArg: 2.31 ± 0.396
3.903AlaSer: 3.903 ± 0.619
4.062AlaThr: 4.062 ± 0.742
3.823AlaVal: 3.823 ± 0.543
1.035AlaTrp: 1.035 ± 0.277
2.23AlaTyr: 2.23 ± 0.462
0.0AlaXaa: 0.0 ± 0.0
Cys
0.558CysAla: 0.558 ± 0.229
0.08CysCys: 0.08 ± 0.081
0.558CysAsp: 0.558 ± 0.209
0.478CysGlu: 0.478 ± 0.236
0.159CysPhe: 0.159 ± 0.113
0.558CysGly: 0.558 ± 0.196
0.08CysHis: 0.08 ± 0.086
0.558CysIle: 0.558 ± 0.175
1.195CysLys: 1.195 ± 0.393
1.035CysLeu: 1.035 ± 0.307
0.08CysMet: 0.08 ± 0.073
0.08CysAsn: 0.08 ± 0.088
0.478CysPro: 0.478 ± 0.214
0.159CysGln: 0.159 ± 0.11
0.478CysArg: 0.478 ± 0.181
0.717CysSer: 0.717 ± 0.214
0.319CysThr: 0.319 ± 0.196
0.319CysVal: 0.319 ± 0.163
0.08CysTrp: 0.08 ± 0.07
0.319CysTyr: 0.319 ± 0.158
0.0CysXaa: 0.0 ± 0.0
Asp
3.584AspAla: 3.584 ± 0.609
0.159AspCys: 0.159 ± 0.12
3.664AspAsp: 3.664 ± 0.629
4.938AspGlu: 4.938 ± 0.692
3.664AspPhe: 3.664 ± 0.544
4.38AspGly: 4.38 ± 0.586
0.796AspHis: 0.796 ± 0.25
3.425AspIle: 3.425 ± 0.451
3.982AspLys: 3.982 ± 0.538
5.336AspLeu: 5.336 ± 0.614
1.911AspMet: 1.911 ± 0.343
2.23AspAsn: 2.23 ± 0.362
1.832AspPro: 1.832 ± 0.393
2.071AspGln: 2.071 ± 0.38
2.867AspArg: 2.867 ± 0.492
3.186AspSer: 3.186 ± 0.416
3.265AspThr: 3.265 ± 0.39
4.141AspVal: 4.141 ± 0.465
0.876AspTrp: 0.876 ± 0.228
1.673AspTyr: 1.673 ± 0.346
0.0AspXaa: 0.0 ± 0.0
Glu
4.938GluAla: 4.938 ± 0.633
0.239GluCys: 0.239 ± 0.133
4.46GluAsp: 4.46 ± 0.652
6.292GluGlu: 6.292 ± 0.927
3.026GluPhe: 3.026 ± 0.427
4.46GluGly: 4.46 ± 0.463
1.195GluHis: 1.195 ± 0.374
7.248GluIle: 7.248 ± 0.888
9.079GluLys: 9.079 ± 0.953
6.451GluLeu: 6.451 ± 0.67
3.823GluMet: 3.823 ± 0.653
3.345GluAsn: 3.345 ± 0.547
2.23GluPro: 2.23 ± 0.403
4.46GluGln: 4.46 ± 0.737
3.425GluArg: 3.425 ± 0.636
4.779GluSer: 4.779 ± 0.503
4.858GluThr: 4.858 ± 0.61
5.734GluVal: 5.734 ± 0.691
1.115GluTrp: 1.115 ± 0.336
3.186GluTyr: 3.186 ± 0.492
0.0GluXaa: 0.0 ± 0.0
Phe
1.991PheAla: 1.991 ± 0.373
0.637PheCys: 0.637 ± 0.203
2.788PheAsp: 2.788 ± 0.543
3.186PheGlu: 3.186 ± 0.453
0.956PhePhe: 0.956 ± 0.296
2.071PheGly: 2.071 ± 0.339
0.717PheHis: 0.717 ± 0.231
3.026PheIle: 3.026 ± 0.416
3.425PheLys: 3.425 ± 0.545
2.549PheLeu: 2.549 ± 0.498
0.876PheMet: 0.876 ± 0.266
1.991PheAsn: 1.991 ± 0.384
0.796PhePro: 0.796 ± 0.27
1.513PheGln: 1.513 ± 0.347
1.593PheArg: 1.593 ± 0.395
2.947PheSer: 2.947 ± 0.576
1.513PheThr: 1.513 ± 0.274
2.469PheVal: 2.469 ± 0.499
0.478PheTrp: 0.478 ± 0.197
1.434PheTyr: 1.434 ± 0.348
0.0PheXaa: 0.0 ± 0.0
Gly
2.947GlyAla: 2.947 ± 0.472
0.717GlyCys: 0.717 ± 0.219
3.265GlyAsp: 3.265 ± 0.476
5.177GlyGlu: 5.177 ± 0.8
2.708GlyPhe: 2.708 ± 0.46
3.743GlyGly: 3.743 ± 0.709
0.796GlyHis: 0.796 ± 0.263
5.416GlyIle: 5.416 ± 0.879
7.248GlyLys: 7.248 ± 0.711
5.256GlyLeu: 5.256 ± 0.905
2.071GlyMet: 2.071 ± 0.571
3.106GlyAsn: 3.106 ± 0.487
0.717GlyPro: 0.717 ± 0.263
2.628GlyGln: 2.628 ± 0.493
3.186GlyArg: 3.186 ± 0.462
3.584GlySer: 3.584 ± 0.637
2.389GlyThr: 2.389 ± 0.566
3.504GlyVal: 3.504 ± 0.497
1.354GlyTrp: 1.354 ± 0.244
2.628GlyTyr: 2.628 ± 0.486
0.0GlyXaa: 0.0 ± 0.0
His
0.956HisAla: 0.956 ± 0.266
0.398HisCys: 0.398 ± 0.187
0.637HisAsp: 0.637 ± 0.227
1.115HisGlu: 1.115 ± 0.29
0.876HisPhe: 0.876 ± 0.25
0.558HisGly: 0.558 ± 0.173
0.398HisHis: 0.398 ± 0.185
1.513HisIle: 1.513 ± 0.275
0.717HisLys: 0.717 ± 0.253
1.673HisLeu: 1.673 ± 0.381
0.478HisMet: 0.478 ± 0.172
0.796HisAsn: 0.796 ± 0.216
0.876HisPro: 0.876 ± 0.239
0.558HisGln: 0.558 ± 0.175
1.035HisArg: 1.035 ± 0.253
0.956HisSer: 0.956 ± 0.273
0.159HisThr: 0.159 ± 0.104
0.876HisVal: 0.876 ± 0.289
0.319HisTrp: 0.319 ± 0.153
0.398HisTyr: 0.398 ± 0.193
0.0HisXaa: 0.0 ± 0.0
Ile
4.301IleAla: 4.301 ± 0.636
0.876IleCys: 0.876 ± 0.236
5.256IleAsp: 5.256 ± 0.61
7.486IleGlu: 7.486 ± 0.786
2.867IlePhe: 2.867 ± 0.402
4.301IleGly: 4.301 ± 0.722
1.354IleHis: 1.354 ± 0.289
3.425IleIle: 3.425 ± 0.718
7.168IleLys: 7.168 ± 0.92
5.416IleLeu: 5.416 ± 0.537
1.195IleMet: 1.195 ± 0.228
3.265IleAsn: 3.265 ± 0.449
2.389IlePro: 2.389 ± 0.404
3.743IleGln: 3.743 ± 0.576
4.062IleArg: 4.062 ± 0.627
4.062IleSer: 4.062 ± 0.559
4.38IleThr: 4.38 ± 0.393
4.221IleVal: 4.221 ± 0.679
0.717IleTrp: 0.717 ± 0.27
2.947IleTyr: 2.947 ± 0.491
0.0IleXaa: 0.0 ± 0.0
Lys
6.371LysAla: 6.371 ± 0.718
0.796LysCys: 0.796 ± 0.447
4.221LysAsp: 4.221 ± 0.456
9.0LysGlu: 9.0 ± 0.833
1.911LysPhe: 1.911 ± 0.316
6.053LysGly: 6.053 ± 0.592
1.274LysHis: 1.274 ± 0.197
6.451LysIle: 6.451 ± 0.641
8.681LysLys: 8.681 ± 1.104
6.61LysLeu: 6.61 ± 0.648
3.504LysMet: 3.504 ± 0.457
3.903LysAsn: 3.903 ± 0.595
2.469LysPro: 2.469 ± 0.51
4.38LysGln: 4.38 ± 0.569
5.416LysArg: 5.416 ± 0.659
5.973LysSer: 5.973 ± 0.765
5.495LysThr: 5.495 ± 0.598
4.779LysVal: 4.779 ± 0.605
1.673LysTrp: 1.673 ± 0.361
4.062LysTyr: 4.062 ± 0.547
0.0LysXaa: 0.0 ± 0.0
Leu
6.053LeuAla: 6.053 ± 0.663
0.558LeuCys: 0.558 ± 0.268
5.177LeuAsp: 5.177 ± 0.537
7.327LeuGlu: 7.327 ± 0.845
2.947LeuPhe: 2.947 ± 0.542
4.779LeuGly: 4.779 ± 0.698
1.354LeuHis: 1.354 ± 0.298
5.336LeuIle: 5.336 ± 0.569
6.929LeuLys: 6.929 ± 0.696
4.858LeuLeu: 4.858 ± 0.681
1.434LeuMet: 1.434 ± 0.351
3.664LeuAsn: 3.664 ± 0.447
2.947LeuPro: 2.947 ± 0.485
3.584LeuGln: 3.584 ± 0.381
3.345LeuArg: 3.345 ± 0.525
6.292LeuSer: 6.292 ± 0.561
5.336LeuThr: 5.336 ± 0.658
3.584LeuVal: 3.584 ± 0.485
1.195LeuTrp: 1.195 ± 0.359
3.026LeuTyr: 3.026 ± 0.473
0.0LeuXaa: 0.0 ± 0.0
Met
1.752MetAla: 1.752 ± 0.341
0.0MetCys: 0.0 ± 0.0
1.673MetAsp: 1.673 ± 0.395
2.23MetGlu: 2.23 ± 0.318
1.195MetPhe: 1.195 ± 0.28
1.354MetGly: 1.354 ± 0.289
0.159MetHis: 0.159 ± 0.112
2.071MetIle: 2.071 ± 0.473
3.425MetLys: 3.425 ± 0.527
2.389MetLeu: 2.389 ± 0.495
0.717MetMet: 0.717 ± 0.223
1.911MetAsn: 1.911 ± 0.381
0.956MetPro: 0.956 ± 0.263
1.115MetGln: 1.115 ± 0.268
1.513MetArg: 1.513 ± 0.376
1.991MetSer: 1.991 ± 0.366
1.354MetThr: 1.354 ± 0.327
0.956MetVal: 0.956 ± 0.231
0.398MetTrp: 0.398 ± 0.159
0.717MetTyr: 0.717 ± 0.25
0.0MetXaa: 0.0 ± 0.0
Asn
2.708AsnAla: 2.708 ± 0.454
0.478AsnCys: 0.478 ± 0.193
1.911AsnAsp: 1.911 ± 0.433
3.504AsnGlu: 3.504 ± 0.55
1.513AsnPhe: 1.513 ± 0.384
3.265AsnGly: 3.265 ± 0.542
0.478AsnHis: 0.478 ± 0.165
2.947AsnIle: 2.947 ± 0.471
3.504AsnLys: 3.504 ± 0.523
3.743AsnLeu: 3.743 ± 0.523
1.195AsnMet: 1.195 ± 0.49
1.832AsnAsn: 1.832 ± 0.495
2.15AsnPro: 2.15 ± 0.417
1.832AsnGln: 1.832 ± 0.38
2.788AsnArg: 2.788 ± 0.603
2.788AsnSer: 2.788 ± 0.428
2.23AsnThr: 2.23 ± 0.379
3.106AsnVal: 3.106 ± 0.528
0.558AsnTrp: 0.558 ± 0.208
1.593AsnTyr: 1.593 ± 0.306
0.0AsnXaa: 0.0 ± 0.0
Pro
2.549ProAla: 2.549 ± 0.381
0.239ProCys: 0.239 ± 0.127
1.911ProAsp: 1.911 ± 0.469
3.186ProGlu: 3.186 ± 0.84
0.876ProPhe: 0.876 ± 0.259
2.708ProGly: 2.708 ± 0.404
0.876ProHis: 0.876 ± 0.26
2.389ProIle: 2.389 ± 0.418
2.788ProLys: 2.788 ± 0.459
2.389ProLeu: 2.389 ± 0.406
0.796ProMet: 0.796 ± 0.255
1.513ProAsn: 1.513 ± 0.301
0.796ProPro: 0.796 ± 0.262
1.035ProGln: 1.035 ± 0.282
0.956ProArg: 0.956 ± 0.28
2.469ProSer: 2.469 ± 0.61
1.673ProThr: 1.673 ± 0.298
1.832ProVal: 1.832 ± 0.489
0.637ProTrp: 0.637 ± 0.19
1.195ProTyr: 1.195 ± 0.285
0.0ProXaa: 0.0 ± 0.0
Gln
3.664GlnAla: 3.664 ± 0.598
0.08GlnCys: 0.08 ± 0.079
2.15GlnAsp: 2.15 ± 0.37
3.743GlnGlu: 3.743 ± 0.683
1.673GlnPhe: 1.673 ± 0.358
2.31GlnGly: 2.31 ± 0.504
1.035GlnHis: 1.035 ± 0.271
2.788GlnIle: 2.788 ± 0.549
4.062GlnLys: 4.062 ± 0.557
3.982GlnLeu: 3.982 ± 0.765
1.434GlnMet: 1.434 ± 0.402
1.752GlnAsn: 1.752 ± 0.343
1.911GlnPro: 1.911 ± 0.503
1.673GlnGln: 1.673 ± 0.413
2.15GlnArg: 2.15 ± 0.383
2.23GlnSer: 2.23 ± 0.361
2.15GlnThr: 2.15 ± 0.335
1.832GlnVal: 1.832 ± 0.375
0.478GlnTrp: 0.478 ± 0.187
0.956GlnTyr: 0.956 ± 0.26
0.0GlnXaa: 0.0 ± 0.0
Arg
1.991ArgAla: 1.991 ± 0.363
0.478ArgCys: 0.478 ± 0.192
3.026ArgAsp: 3.026 ± 0.479
3.743ArgGlu: 3.743 ± 0.644
1.673ArgPhe: 1.673 ± 0.356
2.549ArgGly: 2.549 ± 0.526
1.035ArgHis: 1.035 ± 0.285
3.584ArgIle: 3.584 ± 0.582
6.133ArgLys: 6.133 ± 0.81
4.38ArgLeu: 4.38 ± 0.693
1.115ArgMet: 1.115 ± 0.306
1.991ArgAsn: 1.991 ± 0.349
1.673ArgPro: 1.673 ± 0.339
2.389ArgGln: 2.389 ± 0.406
2.15ArgArg: 2.15 ± 0.376
2.389ArgSer: 2.389 ± 0.421
2.15ArgThr: 2.15 ± 0.372
2.708ArgVal: 2.708 ± 0.508
0.717ArgTrp: 0.717 ± 0.228
1.911ArgTyr: 1.911 ± 0.336
0.0ArgXaa: 0.0 ± 0.0
Ser
4.779SerAla: 4.779 ± 0.923
0.398SerCys: 0.398 ± 0.173
4.062SerAsp: 4.062 ± 0.444
4.858SerGlu: 4.858 ± 0.635
2.469SerPhe: 2.469 ± 0.449
4.221SerGly: 4.221 ± 0.577
0.796SerHis: 0.796 ± 0.26
6.451SerIle: 6.451 ± 0.685
4.221SerLys: 4.221 ± 0.607
5.495SerLeu: 5.495 ± 0.8
1.673SerMet: 1.673 ± 0.373
1.752SerAsn: 1.752 ± 0.37
2.31SerPro: 2.31 ± 0.406
3.026SerGln: 3.026 ± 0.496
2.628SerArg: 2.628 ± 0.427
4.062SerSer: 4.062 ± 0.684
3.425SerThr: 3.425 ± 0.454
4.38SerVal: 4.38 ± 0.769
0.717SerTrp: 0.717 ± 0.211
1.911SerTyr: 1.911 ± 0.295
0.0SerXaa: 0.0 ± 0.0
Thr
3.903ThrAla: 3.903 ± 0.635
0.398ThrCys: 0.398 ± 0.161
2.628ThrAsp: 2.628 ± 0.382
3.664ThrGlu: 3.664 ± 0.457
1.673ThrPhe: 1.673 ± 0.341
4.141ThrGly: 4.141 ± 0.497
0.956ThrHis: 0.956 ± 0.293
4.062ThrIle: 4.062 ± 0.662
4.938ThrLys: 4.938 ± 0.644
4.221ThrLeu: 4.221 ± 0.686
1.434ThrMet: 1.434 ± 0.37
1.911ThrAsn: 1.911 ± 0.317
2.867ThrPro: 2.867 ± 0.473
2.071ThrGln: 2.071 ± 0.379
2.549ThrArg: 2.549 ± 0.371
3.425ThrSer: 3.425 ± 0.45
2.389ThrThr: 2.389 ± 0.448
4.619ThrVal: 4.619 ± 0.623
0.637ThrTrp: 0.637 ± 0.21
1.752ThrTyr: 1.752 ± 0.333
0.0ThrXaa: 0.0 ± 0.0
Val
3.584ValAla: 3.584 ± 0.575
0.717ValCys: 0.717 ± 0.217
3.186ValAsp: 3.186 ± 0.544
4.141ValGlu: 4.141 ± 0.612
3.026ValPhe: 3.026 ± 0.406
3.186ValGly: 3.186 ± 0.707
0.478ValHis: 0.478 ± 0.287
4.301ValIle: 4.301 ± 0.536
5.575ValLys: 5.575 ± 0.591
4.301ValLeu: 4.301 ± 0.548
1.832ValMet: 1.832 ± 0.347
3.026ValAsn: 3.026 ± 0.575
1.832ValPro: 1.832 ± 0.391
1.911ValGln: 1.911 ± 0.371
2.469ValArg: 2.469 ± 0.486
4.858ValSer: 4.858 ± 0.627
4.619ValThr: 4.619 ± 0.513
3.982ValVal: 3.982 ± 0.491
1.673ValTrp: 1.673 ± 0.892
2.15ValTyr: 2.15 ± 0.479
0.0ValXaa: 0.0 ± 0.0
Trp
0.717TrpAla: 0.717 ± 0.225
0.08TrpCys: 0.08 ± 0.07
0.558TrpAsp: 0.558 ± 0.28
1.035TrpGlu: 1.035 ± 0.23
0.956TrpPhe: 0.956 ± 0.259
1.115TrpGly: 1.115 ± 0.264
0.478TrpHis: 0.478 ± 0.18
1.035TrpIle: 1.035 ± 0.367
1.115TrpLys: 1.115 ± 0.344
1.673TrpLeu: 1.673 ± 0.359
0.239TrpMet: 0.239 ± 0.144
1.832TrpAsn: 1.832 ± 1.004
0.319TrpPro: 0.319 ± 0.244
0.637TrpGln: 0.637 ± 0.183
0.319TrpArg: 0.319 ± 0.164
0.796TrpSer: 0.796 ± 0.209
0.637TrpThr: 0.637 ± 0.227
0.956TrpVal: 0.956 ± 0.234
0.239TrpTrp: 0.239 ± 0.123
0.478TrpTyr: 0.478 ± 0.197
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.752TyrAla: 1.752 ± 0.327
0.398TyrCys: 0.398 ± 0.157
2.947TyrAsp: 2.947 ± 0.617
2.947TyrGlu: 2.947 ± 0.511
1.035TyrPhe: 1.035 ± 0.314
2.071TyrGly: 2.071 ± 0.452
0.319TyrHis: 0.319 ± 0.145
2.708TyrIle: 2.708 ± 0.499
3.026TyrLys: 3.026 ± 0.442
2.867TyrLeu: 2.867 ± 0.42
0.796TyrMet: 0.796 ± 0.213
1.115TyrAsn: 1.115 ± 0.335
1.513TyrPro: 1.513 ± 0.371
1.115TyrGln: 1.115 ± 0.251
2.549TyrArg: 2.549 ± 0.504
2.23TyrSer: 2.23 ± 0.404
1.832TyrThr: 1.832 ± 0.307
3.026TyrVal: 3.026 ± 0.43
0.319TyrTrp: 0.319 ± 0.138
1.513TyrTyr: 1.513 ± 0.335
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 65 proteins (12557 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski