Aeropyrum globular virus 1
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 33 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5WQ02|A0A2Z5WQ02_9VIRU Uncharacterized protein OS=Aeropyrum globular virus 1 OX=1932713 PE=4 SV=1
MM1 pKa = 7.48 LLLVAILASVVLPSVAVASAEE22 pKa = 4.12 DD23 pKa = 4.21 PLPLEE28 pKa = 4.39 WGDD31 pKa = 3.68 TGKK34 pKa = 10.24 EE35 pKa = 3.95 LPPGYY40 pKa = 9.82 RR41 pKa = 11.84 VTALTHH47 pKa = 6.46 GIAVAIGGLSGFADD61 pKa = 3.76 AVACVGDD68 pKa = 3.81 YY69 pKa = 11.28 CFGLVGDD76 pKa = 4.83 DD77 pKa = 4.41 GIIPVHH83 pKa = 6.46 VNLFIAGEE91 pKa = 4.07 TRR93 pKa = 11.84 EE94 pKa = 5.58 IDD96 pKa = 3.82 TLWQGMAEE104 pKa = 4.35 YY105 pKa = 10.63 DD106 pKa = 3.75 ADD108 pKa = 3.68 WAFTLHH114 pKa = 6.79 CPGEE118 pKa = 4.6 SIGGLEE124 pKa = 4.55 PGPEE128 pKa = 3.86 EE129 pKa = 3.86 AVLEE133 pKa = 4.56 VEE135 pKa = 4.37 SSQYY139 pKa = 10.58 GIKK142 pKa = 9.98 RR143 pKa = 11.84 YY144 pKa = 10.27 YY145 pKa = 10.75 LVDD148 pKa = 4.2 LSQGVSPDD156 pKa = 2.97 LKK158 pKa = 10.38 YY159 pKa = 10.54 FYY161 pKa = 10.41 EE162 pKa = 4.24 STPGVTSWVEE172 pKa = 3.63 FTDD175 pKa = 3.73 PEE177 pKa = 4.3 KK178 pKa = 11.43 VKK180 pKa = 11.02 DD181 pKa = 4.22 CGGDD185 pKa = 3.63 YY186 pKa = 10.55 QGEE189 pKa = 4.2 PPSGGEE195 pKa = 3.94 GNRR198 pKa = 11.84 PQGGGLLDD206 pKa = 4.76 LNMTVDD212 pKa = 4.48 LTWLALGLVAVGFLLILLKK231 pKa = 10.55 RR232 pKa = 11.84 WW233 pKa = 3.21
Molecular weight: 24.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.797
IPC2_protein 4.037
IPC_protein 3.999
Toseland 3.808
ProMoST 4.139
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.872
Rodwell 3.834
Grimsley 3.719
Solomon 3.961
Lehninger 3.923
Nozaki 4.088
DTASelect 4.266
Thurlkill 3.846
EMBOSS 3.884
Sillero 4.113
Patrickios 1.163
IPC_peptide 3.973
IPC2_peptide 4.101
IPC2.peptide.svr19 4.006
Protein with the highest isoelectric point:
>tr|A0A2Z5WQ25|A0A2Z5WQ25_9VIRU Putative Glutamyl-tRNAGlu reductase OS=Aeropyrum globular virus 1 OX=1932713 PE=4 SV=1
MM1 pKa = 7.56 AEE3 pKa = 3.93 VVIAYY8 pKa = 9.6 SPHH11 pKa = 4.35 TRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 AEE17 pKa = 3.65 ALAWFFQRR25 pKa = 11.84 LGLRR29 pKa = 11.84 VVLRR33 pKa = 11.84 EE34 pKa = 4.16 DD35 pKa = 3.17 GGEE38 pKa = 4.24 GVSVKK43 pKa = 10.92 YY44 pKa = 10.8 GGVWWLDD51 pKa = 3.02 PDD53 pKa = 3.48 EE54 pKa = 5.47 AIRR57 pKa = 11.84 HH58 pKa = 4.7 IVSRR62 pKa = 11.84 GWGNFNPRR70 pKa = 11.84 LKK72 pKa = 10.11 TSVNGGGRR80 pKa = 11.84 PLL82 pKa = 3.67
Molecular weight: 9.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.319
IPC2_protein 9.531
IPC_protein 10.526
Toseland 10.452
ProMoST 10.262
Dawson 10.599
Bjellqvist 10.35
Wikipedia 10.847
Rodwell 10.628
Grimsley 10.672
Solomon 10.745
Lehninger 10.701
Nozaki 10.409
DTASelect 10.365
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.526
Patrickios 10.452
IPC_peptide 10.73
IPC2_peptide 9.268
IPC2.peptide.svr19 8.619
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
33
0
33
5557
61
354
168.4
18.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.124 ± 0.542
0.936 ± 0.152
4.877 ± 0.305
6.874 ± 0.466
2.789 ± 0.252
8.188 ± 0.529
1.584 ± 0.23
4.949 ± 0.223
4.229 ± 0.641
11.139 ± 0.535
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.159 ± 0.197
2.375 ± 0.285
4.499 ± 0.276
2.573 ± 0.195
7.054 ± 0.611
5.201 ± 0.367
5.615 ± 0.693
9.232 ± 0.454
1.997 ± 0.251
4.607 ± 0.387
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here