Microbacterium phage KaiHaiDragon

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Quhwahvirus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345MHZ5|A0A345MHZ5_9CAUD Uncharacterized protein OS=Microbacterium phage KaiHaiDragon OX=2283289 GN=64 PE=4 SV=1
MM1 pKa = 7.83RR2 pKa = 11.84IKK4 pKa = 10.82VKK6 pKa = 10.61ASQGGGMFGGGPSSEE21 pKa = 4.15VVIEE25 pKa = 4.14GSEE28 pKa = 3.74ADD30 pKa = 3.59AAQGTISAMMILAQNGIGEE49 pKa = 4.29YY50 pKa = 10.19PGDD53 pKa = 4.88DD54 pKa = 4.36EE55 pKa = 5.0EE56 pKa = 5.46TDD58 pKa = 3.56DD59 pKa = 5.57DD60 pKa = 4.69SPEE63 pKa = 3.95EE64 pKa = 5.26GYY66 pKa = 8.73TEE68 pKa = 4.66PDD70 pKa = 3.52DD71 pKa = 4.5VPAAGWKK78 pKa = 10.17EE79 pKa = 4.01DD80 pKa = 3.87DD81 pKa = 5.03PSANWEE87 pKa = 4.48AGDD90 pKa = 4.0EE91 pKa = 4.41SPSTEE96 pKa = 3.79EE97 pKa = 4.32HH98 pKa = 6.47GSNPGDD104 pKa = 3.36AVRR107 pKa = 11.84IVWNGDD113 pKa = 2.75QSSRR117 pKa = 11.84LFIDD121 pKa = 5.28SGVCWVSPAVAGLYY135 pKa = 9.79SEE137 pKa = 4.56PMTVEE142 pKa = 4.09YY143 pKa = 10.22NASDD147 pKa = 3.13IAVYY151 pKa = 10.1VVAEE155 pKa = 4.24VQKK158 pKa = 11.06HH159 pKa = 4.11VGG161 pKa = 3.26

Molecular weight: 16.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345MHV8|A0A345MHV8_9CAUD Uncharacterized protein OS=Microbacterium phage KaiHaiDragon OX=2283289 GN=27 PE=4 SV=1
MM1 pKa = 7.5GCACNKK7 pKa = 9.66QKK9 pKa = 11.41SNGLAAKK16 pKa = 9.89RR17 pKa = 11.84EE18 pKa = 4.13EE19 pKa = 4.39SATTSTSAKK28 pKa = 9.8SDD30 pKa = 3.58TEE32 pKa = 4.1SRR34 pKa = 11.84QATTASRR41 pKa = 11.84LSAPTSRR48 pKa = 11.84RR49 pKa = 11.84QSFALEE55 pKa = 4.12SGGRR59 pKa = 11.84TASFGSRR66 pKa = 11.84LEE68 pKa = 3.93RR69 pKa = 11.84DD70 pKa = 3.0AAAARR75 pKa = 11.84SRR77 pKa = 11.84GG78 pKa = 3.52

Molecular weight: 8.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

16906

32

955

183.8

19.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.079 ± 0.393

0.852 ± 0.113

6.743 ± 0.363

6.489 ± 0.376

2.455 ± 0.136

8.737 ± 0.286

2.017 ± 0.192

4.525 ± 0.238

2.484 ± 0.187

7.589 ± 0.406

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.396 ± 0.113

2.49 ± 0.167

6.075 ± 0.295

3.064 ± 0.165

7.139 ± 0.381

5.868 ± 0.26

7.009 ± 0.293

7.477 ± 0.244

1.893 ± 0.148

2.62 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski