Amino acid dipepetide frequency for Acutalibacter sp. 1XD8-33

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.357AlaAla: 10.357 ± 0.134
1.437AlaCys: 1.437 ± 0.043
4.481AlaAsp: 4.481 ± 0.065
6.379AlaGlu: 6.379 ± 0.096
3.47AlaPhe: 3.47 ± 0.069
7.839AlaGly: 7.839 ± 0.105
1.466AlaHis: 1.466 ± 0.045
4.429AlaIle: 4.429 ± 0.08
4.27AlaLys: 4.27 ± 0.083
9.475AlaLeu: 9.475 ± 0.106
2.651AlaMet: 2.651 ± 0.052
2.347AlaAsn: 2.347 ± 0.051
3.398AlaPro: 3.398 ± 0.068
3.795AlaGln: 3.795 ± 0.056
4.818AlaArg: 4.818 ± 0.084
4.32AlaSer: 4.32 ± 0.077
3.38AlaThr: 3.38 ± 0.069
7.42AlaVal: 7.42 ± 0.094
0.892AlaTrp: 0.892 ± 0.038
2.828AlaTyr: 2.828 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
1.427CysAla: 1.427 ± 0.039
0.362CysCys: 0.362 ± 0.02
0.772CysAsp: 0.772 ± 0.03
0.859CysGlu: 0.859 ± 0.03
0.642CysPhe: 0.642 ± 0.028
1.926CysGly: 1.926 ± 0.049
0.331CysHis: 0.331 ± 0.02
0.706CysIle: 0.706 ± 0.033
0.766CysLys: 0.766 ± 0.031
1.382CysLeu: 1.382 ± 0.041
0.39CysMet: 0.39 ± 0.018
0.479CysAsn: 0.479 ± 0.023
0.902CysPro: 0.902 ± 0.032
0.632CysGln: 0.632 ± 0.026
1.204CysArg: 1.204 ± 0.036
0.843CysSer: 0.843 ± 0.032
0.77CysThr: 0.77 ± 0.03
1.105CysVal: 1.105 ± 0.034
0.227CysTrp: 0.227 ± 0.015
0.596CysTyr: 0.596 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
4.027AspAla: 4.027 ± 0.064
0.932AspCys: 0.932 ± 0.034
2.312AspAsp: 2.312 ± 0.066
3.749AspGlu: 3.749 ± 0.079
2.738AspPhe: 2.738 ± 0.054
4.88AspGly: 4.88 ± 0.079
0.832AspHis: 0.832 ± 0.028
3.365AspIle: 3.365 ± 0.062
2.673AspLys: 2.673 ± 0.056
4.972AspLeu: 4.972 ± 0.069
1.627AspMet: 1.627 ± 0.042
1.7AspAsn: 1.7 ± 0.042
2.683AspPro: 2.683 ± 0.063
1.568AspGln: 1.568 ± 0.046
3.103AspArg: 3.103 ± 0.06
3.11AspSer: 3.11 ± 0.055
2.854AspThr: 2.854 ± 0.066
3.478AspVal: 3.478 ± 0.065
0.803AspTrp: 0.803 ± 0.027
2.291AspTyr: 2.291 ± 0.049
0.0AspXaa: 0.0 ± 0.0
Glu
6.529GluAla: 6.529 ± 0.089
0.822GluCys: 0.822 ± 0.03
4.075GluAsp: 4.075 ± 0.072
6.355GluGlu: 6.355 ± 0.1
2.495GluPhe: 2.495 ± 0.056
4.875GluGly: 4.875 ± 0.074
1.236GluHis: 1.236 ± 0.037
4.769GluIle: 4.769 ± 0.079
5.187GluLys: 5.187 ± 0.082
7.041GluLeu: 7.041 ± 0.094
2.1GluMet: 2.1 ± 0.048
3.596GluAsn: 3.596 ± 0.063
2.619GluPro: 2.619 ± 0.07
2.918GluGln: 2.918 ± 0.06
4.515GluArg: 4.515 ± 0.083
3.645GluSer: 3.645 ± 0.059
3.513GluThr: 3.513 ± 0.06
4.002GluVal: 4.002 ± 0.062
0.685GluTrp: 0.685 ± 0.026
2.647GluTyr: 2.647 ± 0.054
0.0GluXaa: 0.0 ± 0.0
Phe
3.341PheAla: 3.341 ± 0.055
0.893PheCys: 0.893 ± 0.033
2.338PheAsp: 2.338 ± 0.056
2.264PheGlu: 2.264 ± 0.048
1.863PhePhe: 1.863 ± 0.049
3.122PheGly: 3.122 ± 0.061
0.824PheHis: 0.824 ± 0.025
1.957PheIle: 1.957 ± 0.049
1.375PheLys: 1.375 ± 0.044
4.106PheLeu: 4.106 ± 0.072
0.912PheMet: 0.912 ± 0.032
1.172PheAsn: 1.172 ± 0.038
1.739PhePro: 1.739 ± 0.042
1.812PheGln: 1.812 ± 0.046
2.162PheArg: 2.162 ± 0.055
3.264PheSer: 3.264 ± 0.063
2.41PheThr: 2.41 ± 0.056
2.581PheVal: 2.581 ± 0.059
0.512PheTrp: 0.512 ± 0.024
1.493PheTyr: 1.493 ± 0.039
0.0PheXaa: 0.0 ± 0.0
Gly
6.492GlyAla: 6.492 ± 0.094
1.333GlyCys: 1.333 ± 0.038
4.004GlyAsp: 4.004 ± 0.067
5.929GlyGlu: 5.929 ± 0.095
3.389GlyPhe: 3.389 ± 0.052
6.58GlyGly: 6.58 ± 0.106
1.341GlyHis: 1.341 ± 0.039
4.803GlyIle: 4.803 ± 0.078
4.981GlyLys: 4.981 ± 0.07
7.261GlyLeu: 7.261 ± 0.086
2.449GlyMet: 2.449 ± 0.051
2.724GlyAsn: 2.724 ± 0.06
2.152GlyPro: 2.152 ± 0.054
2.868GlyGln: 2.868 ± 0.062
4.747GlyArg: 4.747 ± 0.074
4.267GlySer: 4.267 ± 0.072
4.001GlyThr: 4.001 ± 0.087
5.782GlyVal: 5.782 ± 0.088
1.03GlyTrp: 1.03 ± 0.033
3.179GlyTyr: 3.179 ± 0.054
0.0GlyXaa: 0.0 ± 0.0
His
1.138HisAla: 1.138 ± 0.039
0.385HisCys: 0.385 ± 0.02
0.807HisAsp: 0.807 ± 0.028
0.956HisGlu: 0.956 ± 0.036
0.804HisPhe: 0.804 ± 0.027
1.491HisGly: 1.491 ± 0.047
0.401HisHis: 0.401 ± 0.026
1.191HisIle: 1.191 ± 0.037
0.801HisLys: 0.801 ± 0.029
1.615HisLeu: 1.615 ± 0.041
0.471HisMet: 0.471 ± 0.023
0.681HisAsn: 0.681 ± 0.028
0.969HisPro: 0.969 ± 0.036
0.645HisGln: 0.645 ± 0.025
1.113HisArg: 1.113 ± 0.031
1.126HisSer: 1.126 ± 0.038
0.962HisThr: 0.962 ± 0.033
1.04HisVal: 1.04 ± 0.033
0.271HisTrp: 0.271 ± 0.018
0.723HisTyr: 0.723 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
5.042IleAla: 5.042 ± 0.075
0.941IleCys: 0.941 ± 0.034
3.23IleAsp: 3.23 ± 0.066
3.445IleGlu: 3.445 ± 0.064
2.264IlePhe: 2.264 ± 0.054
3.865IleGly: 3.865 ± 0.076
1.028IleHis: 1.028 ± 0.036
3.044IleIle: 3.044 ± 0.068
2.45IleLys: 2.45 ± 0.049
5.552IleLeu: 5.552 ± 0.081
1.308IleMet: 1.308 ± 0.035
1.912IleAsn: 1.912 ± 0.046
2.626IlePro: 2.626 ± 0.053
2.071IleGln: 2.071 ± 0.047
3.221IleArg: 3.221 ± 0.054
3.879IleSer: 3.879 ± 0.066
3.293IleThr: 3.293 ± 0.065
3.572IleVal: 3.572 ± 0.056
0.559IleTrp: 0.559 ± 0.023
1.848IleTyr: 1.848 ± 0.043
0.0IleXaa: 0.0 ± 0.0
Lys
4.875LysAla: 4.875 ± 0.079
0.614LysCys: 0.614 ± 0.026
2.75LysAsp: 2.75 ± 0.059
4.299LysGlu: 4.299 ± 0.07
1.557LysPhe: 1.557 ± 0.046
3.489LysGly: 3.489 ± 0.058
0.786LysHis: 0.786 ± 0.027
3.158LysIle: 3.158 ± 0.06
3.798LysLys: 3.798 ± 0.07
4.786LysLeu: 4.786 ± 0.07
1.528LysMet: 1.528 ± 0.041
2.225LysAsn: 2.225 ± 0.057
2.19LysPro: 2.19 ± 0.049
1.779LysGln: 1.779 ± 0.052
3.181LysArg: 3.181 ± 0.062
2.881LysSer: 2.881 ± 0.054
3.023LysThr: 3.023 ± 0.065
3.003LysVal: 3.003 ± 0.055
0.564LysTrp: 0.564 ± 0.026
1.919LysTyr: 1.919 ± 0.048
0.0LysXaa: 0.0 ± 0.0
Leu
8.917LeuAla: 8.917 ± 0.107
2.084LeuCys: 2.084 ± 0.052
5.64LeuAsp: 5.64 ± 0.089
7.085LeuGlu: 7.085 ± 0.096
3.952LeuPhe: 3.952 ± 0.078
7.272LeuGly: 7.272 ± 0.097
1.712LeuHis: 1.712 ± 0.043
4.68LeuIle: 4.68 ± 0.076
4.742LeuLys: 4.742 ± 0.076
9.924LeuLeu: 9.924 ± 0.153
2.385LeuMet: 2.385 ± 0.055
3.274LeuAsn: 3.274 ± 0.054
4.828LeuPro: 4.828 ± 0.081
2.985LeuGln: 2.985 ± 0.065
5.811LeuArg: 5.811 ± 0.097
6.679LeuSer: 6.679 ± 0.09
5.235LeuThr: 5.235 ± 0.083
5.614LeuVal: 5.614 ± 0.076
1.081LeuTrp: 1.081 ± 0.036
3.166LeuTyr: 3.166 ± 0.064
0.0LeuXaa: 0.0 ± 0.0
Met
2.654MetAla: 2.654 ± 0.056
0.322MetCys: 0.322 ± 0.017
1.729MetAsp: 1.729 ± 0.039
2.486MetGlu: 2.486 ± 0.056
0.844MetPhe: 0.844 ± 0.033
2.115MetGly: 2.115 ± 0.047
0.369MetHis: 0.369 ± 0.019
1.307MetIle: 1.307 ± 0.038
1.808MetLys: 1.808 ± 0.04
2.558MetLeu: 2.558 ± 0.044
0.731MetMet: 0.731 ± 0.025
1.247MetAsn: 1.247 ± 0.037
1.172MetPro: 1.172 ± 0.035
0.89MetGln: 0.89 ± 0.028
1.543MetArg: 1.543 ± 0.042
1.526MetSer: 1.526 ± 0.042
1.401MetThr: 1.401 ± 0.035
1.657MetVal: 1.657 ± 0.036
0.219MetTrp: 0.219 ± 0.017
0.705MetTyr: 0.705 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
2.992AsnAla: 2.992 ± 0.067
0.613AsnCys: 0.613 ± 0.024
1.632AsnAsp: 1.632 ± 0.045
1.96AsnGlu: 1.96 ± 0.04
1.41AsnPhe: 1.41 ± 0.039
3.125AsnGly: 3.125 ± 0.061
0.66AsnHis: 0.66 ± 0.029
2.265AsnIle: 2.265 ± 0.057
1.437AsnLys: 1.437 ± 0.043
3.363AsnLeu: 3.363 ± 0.055
0.959AsnMet: 0.959 ± 0.028
1.203AsnAsn: 1.203 ± 0.038
2.081AsnPro: 2.081 ± 0.049
1.312AsnGln: 1.312 ± 0.039
2.155AsnArg: 2.155 ± 0.049
2.022AsnSer: 2.022 ± 0.047
1.862AsnThr: 1.862 ± 0.052
2.213AsnVal: 2.213 ± 0.056
0.428AsnTrp: 0.428 ± 0.024
1.357AsnTyr: 1.357 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
4.094ProAla: 4.094 ± 0.083
0.623ProCys: 0.623 ± 0.026
2.772ProAsp: 2.772 ± 0.064
4.607ProGlu: 4.607 ± 0.075
1.678ProPhe: 1.678 ± 0.042
3.597ProGly: 3.597 ± 0.075
0.77ProHis: 0.77 ± 0.033
1.985ProIle: 1.985 ± 0.044
2.015ProLys: 2.015 ± 0.049
3.573ProLeu: 3.573 ± 0.073
1.063ProMet: 1.063 ± 0.033
1.321ProAsn: 1.321 ± 0.041
1.847ProPro: 1.847 ± 0.058
1.874ProGln: 1.874 ± 0.044
1.867ProArg: 1.867 ± 0.046
2.354ProSer: 2.354 ± 0.061
2.157ProThr: 2.157 ± 0.074
3.495ProVal: 3.495 ± 0.068
0.538ProTrp: 0.538 ± 0.027
1.493ProTyr: 1.493 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
3.59GlnAla: 3.59 ± 0.06
0.456GlnCys: 0.456 ± 0.02
1.779GlnAsp: 1.779 ± 0.04
3.249GlnGlu: 3.249 ± 0.063
1.19GlnPhe: 1.19 ± 0.035
2.889GlnGly: 2.889 ± 0.058
0.578GlnHis: 0.578 ± 0.024
2.109GlnIle: 2.109 ± 0.046
2.497GlnLys: 2.497 ± 0.049
3.35GlnLeu: 3.35 ± 0.066
1.116GlnMet: 1.116 ± 0.032
1.56GlnAsn: 1.56 ± 0.042
1.476GlnPro: 1.476 ± 0.047
1.416GlnGln: 1.416 ± 0.047
2.347GlnArg: 2.347 ± 0.051
2.0GlnSer: 2.0 ± 0.048
1.727GlnThr: 1.727 ± 0.051
2.481GlnVal: 2.481 ± 0.051
0.46GlnTrp: 0.46 ± 0.022
1.299GlnTyr: 1.299 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
4.649ArgAla: 4.649 ± 0.074
0.814ArgCys: 0.814 ± 0.029
3.007ArgAsp: 3.007 ± 0.052
5.358ArgGlu: 5.358 ± 0.11
2.505ArgPhe: 2.505 ± 0.054
3.73ArgGly: 3.73 ± 0.069
1.145ArgHis: 1.145 ± 0.036
3.197ArgIle: 3.197 ± 0.057
3.263ArgLys: 3.263 ± 0.059
6.014ArgLeu: 6.014 ± 0.094
1.81ArgMet: 1.81 ± 0.042
2.06ArgAsn: 2.06 ± 0.044
2.373ArgPro: 2.373 ± 0.054
2.577ArgGln: 2.577 ± 0.056
4.258ArgArg: 4.258 ± 0.086
2.709ArgSer: 2.709 ± 0.051
2.591ArgThr: 2.591 ± 0.055
3.715ArgVal: 3.715 ± 0.062
0.727ArgTrp: 0.727 ± 0.027
2.199ArgTyr: 2.199 ± 0.05
0.0ArgXaa: 0.0 ± 0.0
Ser
5.207SerAla: 5.207 ± 0.085
0.86SerCys: 0.86 ± 0.032
2.847SerAsp: 2.847 ± 0.065
3.292SerGlu: 3.292 ± 0.059
2.458SerPhe: 2.458 ± 0.055
5.611SerGly: 5.611 ± 0.091
1.063SerHis: 1.063 ± 0.032
3.184SerIle: 3.184 ± 0.067
2.518SerLys: 2.518 ± 0.053
5.572SerLeu: 5.572 ± 0.075
1.617SerMet: 1.617 ± 0.045
1.702SerAsn: 1.702 ± 0.042
2.854SerPro: 2.854 ± 0.067
2.448SerGln: 2.448 ± 0.054
3.49SerArg: 3.49 ± 0.066
3.322SerSer: 3.322 ± 0.076
2.778SerThr: 2.778 ± 0.059
4.002SerVal: 4.002 ± 0.081
0.704SerTrp: 0.704 ± 0.031
2.124SerTyr: 2.124 ± 0.05
0.0SerXaa: 0.0 ± 0.0
Thr
5.109ThrAla: 5.109 ± 0.087
0.638ThrCys: 0.638 ± 0.026
2.806ThrAsp: 2.806 ± 0.054
3.318ThrGlu: 3.318 ± 0.065
1.934ThrPhe: 1.934 ± 0.046
4.762ThrGly: 4.762 ± 0.081
0.915ThrHis: 0.915 ± 0.03
2.889ThrIle: 2.889 ± 0.059
2.09ThrLys: 2.09 ± 0.057
4.958ThrLeu: 4.958 ± 0.081
1.236ThrMet: 1.236 ± 0.035
1.496ThrAsn: 1.496 ± 0.041
2.871ThrPro: 2.871 ± 0.088
1.691ThrGln: 1.691 ± 0.042
2.433ThrArg: 2.433 ± 0.049
2.533ThrSer: 2.533 ± 0.061
2.385ThrThr: 2.385 ± 0.062
4.668ThrVal: 4.668 ± 0.084
0.513ThrTrp: 0.513 ± 0.026
1.67ThrTyr: 1.67 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
5.101ValAla: 5.101 ± 0.076
1.3ValCys: 1.3 ± 0.039
3.964ValAsp: 3.964 ± 0.067
4.85ValGlu: 4.85 ± 0.08
2.886ValPhe: 2.886 ± 0.066
4.391ValGly: 4.391 ± 0.07
1.114ValHis: 1.114 ± 0.039
3.646ValIle: 3.646 ± 0.065
3.533ValLys: 3.533 ± 0.068
6.959ValLeu: 6.959 ± 0.098
1.794ValMet: 1.794 ± 0.041
2.495ValAsn: 2.495 ± 0.058
3.041ValPro: 3.041 ± 0.051
2.164ValGln: 2.164 ± 0.049
3.773ValArg: 3.773 ± 0.059
4.494ValSer: 4.494 ± 0.073
3.913ValThr: 3.913 ± 0.084
4.503ValVal: 4.503 ± 0.078
0.757ValTrp: 0.757 ± 0.026
2.403ValTyr: 2.403 ± 0.051
0.0ValXaa: 0.0 ± 0.0
Trp
1.011TrpAla: 1.011 ± 0.033
0.217TrpCys: 0.217 ± 0.018
0.682TrpAsp: 0.682 ± 0.028
0.925TrpGlu: 0.925 ± 0.029
0.478TrpPhe: 0.478 ± 0.019
0.926TrpGly: 0.926 ± 0.033
0.266TrpHis: 0.266 ± 0.017
0.5TrpIle: 0.5 ± 0.024
0.619TrpLys: 0.619 ± 0.025
1.133TrpLeu: 1.133 ± 0.04
0.296TrpMet: 0.296 ± 0.017
0.508TrpAsn: 0.508 ± 0.026
0.336TrpPro: 0.336 ± 0.018
0.529TrpGln: 0.529 ± 0.025
0.684TrpArg: 0.684 ± 0.026
0.55TrpSer: 0.55 ± 0.024
0.575TrpThr: 0.575 ± 0.025
0.689TrpVal: 0.689 ± 0.026
0.15TrpTrp: 0.15 ± 0.013
0.443TrpTyr: 0.443 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.896TyrAla: 2.896 ± 0.062
0.673TyrCys: 0.673 ± 0.027
2.073TyrAsp: 2.073 ± 0.049
2.258TyrGlu: 2.258 ± 0.044
1.586TyrPhe: 1.586 ± 0.046
2.826TyrGly: 2.826 ± 0.053
0.753TyrHis: 0.753 ± 0.031
1.962TyrIle: 1.962 ± 0.044
1.582TyrLys: 1.582 ± 0.047
3.471TyrLeu: 3.471 ± 0.068
0.84TyrMet: 0.84 ± 0.031
1.35TyrAsn: 1.35 ± 0.039
1.565TyrPro: 1.565 ± 0.042
1.595TyrGln: 1.595 ± 0.046
2.276TyrArg: 2.276 ± 0.051
2.131TyrSer: 2.131 ± 0.041
2.082TyrThr: 2.082 ± 0.044
2.062TyrVal: 2.062 ± 0.056
0.399TyrTrp: 0.399 ± 0.022
1.371TyrTyr: 1.371 ± 0.034
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3386 proteins (976833 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski