Weissella koreensis (strain KACC 15510)
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1346 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F8HXX9|F8HXX9_WEIKK PST family polysaccharide transporter OS=Weissella koreensis (strain KACC 15510) OX=1045854 GN=WKK_00395 PE=4 SV=1
MM1 pKa = 7.07 QSYY4 pKa = 10.0 AEE6 pKa = 4.32 KK7 pKa = 10.39 MLDD10 pKa = 3.68 EE11 pKa = 5.11 LDD13 pKa = 4.12 GGQLEE18 pKa = 4.17 AAKK21 pKa = 10.48 KK22 pKa = 10.51 SFALSLRR29 pKa = 11.84 HH30 pKa = 7.01 DD31 pKa = 4.66 DD32 pKa = 5.28 DD33 pKa = 3.98 EE34 pKa = 5.23 TIHH37 pKa = 6.93 SLAEE41 pKa = 3.55 EE42 pKa = 4.82 LYY44 pKa = 11.02 ALGFSTNAKK53 pKa = 9.63 RR54 pKa = 11.84 AYY56 pKa = 9.92 QKK58 pKa = 11.32 LLDD61 pKa = 4.59 KK62 pKa = 11.52 YY63 pKa = 10.22 PDD65 pKa = 3.47 EE66 pKa = 5.06 DD67 pKa = 3.73 VLRR70 pKa = 11.84 TEE72 pKa = 4.41 LAEE75 pKa = 3.94 ISISEE80 pKa = 4.2 DD81 pKa = 3.31 QSDD84 pKa = 3.5 EE85 pKa = 3.81 ALAYY89 pKa = 9.09 LIPIQSDD96 pKa = 3.5 SEE98 pKa = 4.55 AYY100 pKa = 10.63 LEE102 pKa = 4.31 ALLVYY107 pKa = 10.72 ADD109 pKa = 5.11 LYY111 pKa = 10.73 QSEE114 pKa = 4.65 GLLEE118 pKa = 4.09 AAEE121 pKa = 4.27 NKK123 pKa = 8.78 LTEE126 pKa = 4.69 AYY128 pKa = 10.14 QLAPDD133 pKa = 3.67 EE134 pKa = 4.92 LVIQFALAEE143 pKa = 4.18 FLFEE147 pKa = 4.62 LGRR150 pKa = 11.84 YY151 pKa = 8.96 SEE153 pKa = 5.72 AIPFYY158 pKa = 10.9 RR159 pKa = 11.84 NLIVQGEE166 pKa = 4.39 TQISGIDD173 pKa = 3.41 LVSRR177 pKa = 11.84 IGVAYY182 pKa = 10.56 AMLGDD187 pKa = 3.67 QNRR190 pKa = 11.84 ALGYY194 pKa = 10.44 LEE196 pKa = 4.28 QIKK199 pKa = 10.23 DD200 pKa = 3.51 ANLTPEE206 pKa = 3.87 VRR208 pKa = 11.84 FQLGMLYY215 pKa = 10.78 ANDD218 pKa = 3.92 EE219 pKa = 4.25 EE220 pKa = 4.74 TRR222 pKa = 11.84 DD223 pKa = 3.45 QGIEE227 pKa = 3.93 AFEE230 pKa = 4.02 KK231 pKa = 10.71 LIEE234 pKa = 4.58 IDD236 pKa = 3.29 PSYY239 pKa = 11.28 AGVYY243 pKa = 9.77 VPLGQLYY250 pKa = 7.72 EE251 pKa = 4.11 QKK253 pKa = 10.8 QMPKK257 pKa = 9.95 DD258 pKa = 3.29 ALGIYY263 pKa = 8.9 EE264 pKa = 5.05 AGLAVDD270 pKa = 4.1 QFNEE274 pKa = 3.89 AGYY277 pKa = 11.17 LNATRR282 pKa = 11.84 VAIQLNEE289 pKa = 3.87 NDD291 pKa = 3.63 QAEE294 pKa = 4.21 QLYY297 pKa = 10.5 QKK299 pKa = 10.41 GLKK302 pKa = 9.54 NLPDD306 pKa = 3.82 SQNLISNYY314 pKa = 9.6 SQMLVDD320 pKa = 3.76 TEE322 pKa = 4.47 QYY324 pKa = 9.29 MEE326 pKa = 4.64 QINFLNQYY334 pKa = 9.69 VSADD338 pKa = 3.89 DD339 pKa = 4.57 EE340 pKa = 4.89 FEE342 pKa = 5.03 LDD344 pKa = 3.94 PKK346 pKa = 10.74 EE347 pKa = 4.21 YY348 pKa = 10.05 WNLAQSYY355 pKa = 8.45 TEE357 pKa = 3.96 LEE359 pKa = 4.29 RR360 pKa = 11.84 YY361 pKa = 9.34 EE362 pKa = 4.41 MADD365 pKa = 3.44 QYY367 pKa = 10.14 WQAAVPFFNEE377 pKa = 3.13 NSIFLKK383 pKa = 10.04 EE384 pKa = 3.81 AIYY387 pKa = 10.13 YY388 pKa = 9.66 FRR390 pKa = 11.84 EE391 pKa = 4.43 AGNHH395 pKa = 5.26 EE396 pKa = 4.52 MLSDD400 pKa = 4.31 LLNKK404 pKa = 9.56 YY405 pKa = 9.23 VQLNPEE411 pKa = 4.54 DD412 pKa = 4.94 FEE414 pKa = 4.39 MAQMEE419 pKa = 4.85 SEE421 pKa = 4.26 INDD424 pKa = 3.65 LL425 pKa = 4.37
Molecular weight: 48.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.783
IPC2_protein 4.012
IPC_protein 3.986
Toseland 3.795
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.808
Rodwell 3.808
Grimsley 3.706
Solomon 3.935
Lehninger 3.884
Nozaki 4.037
DTASelect 4.19
Thurlkill 3.808
EMBOSS 3.821
Sillero 4.088
Patrickios 1.443
IPC_peptide 3.935
IPC2_peptide 4.075
IPC2.peptide.svr19 3.991
Protein with the highest isoelectric point:
>tr|F8I1A0|F8I1A0_WEIKK Alkaline phosphatase superfamily protein OS=Weissella koreensis (strain KACC 15510) OX=1045854 GN=WKK_03885 PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.69 QPKK8 pKa = 8.34 KK9 pKa = 7.47 RR10 pKa = 11.84 HH11 pKa = 5.71 RR12 pKa = 11.84 EE13 pKa = 3.7 RR14 pKa = 11.84 VHH16 pKa = 6.23 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 KK29 pKa = 8.01 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.39 GRR39 pKa = 11.84 KK40 pKa = 8.52 VLSAA44 pKa = 4.05
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 10.862
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.193
Grimsley 12.486
Solomon 12.925
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.93
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.043
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1346
0
1346
404084
30
2007
300.2
33.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.532 ± 0.073
0.185 ± 0.01
5.89 ± 0.07
6.045 ± 0.065
4.256 ± 0.054
6.595 ± 0.073
1.918 ± 0.028
7.841 ± 0.079
6.384 ± 0.075
9.945 ± 0.089
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.936 ± 0.04
5.405 ± 0.07
3.38 ± 0.036
4.72 ± 0.068
3.859 ± 0.053
5.927 ± 0.118
5.728 ± 0.047
6.993 ± 0.055
1.076 ± 0.027
3.388 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here