Ceriporiopsis subvermispora (strain B) (White-rot fungus) (Gelatoporia subvermispora)
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12078 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M2PRF8|M2PRF8_CERS8 Uncharacterized protein OS=Ceriporiopsis subvermispora (strain B) OX=914234 GN=CERSUDRAFT_81950 PE=3 SV=1
MM1 pKa = 7.35 PAEE4 pKa = 4.21 LASPQRR10 pKa = 11.84 PQSLSTMARR19 pKa = 11.84 SKK21 pKa = 10.66 LVEE24 pKa = 4.31 VGSGARR30 pKa = 11.84 DD31 pKa = 3.55 FSLHH35 pKa = 5.86 RR36 pKa = 11.84 WVLLKK41 pKa = 11.06 NSFTRR46 pKa = 11.84 SHH48 pKa = 7.36 PLNAATVAAAAPAPAQADD66 pKa = 3.49 VNYY69 pKa = 10.12 VYY71 pKa = 10.93 RR72 pKa = 11.84 HH73 pKa = 5.11 GAAPASEE80 pKa = 4.14 QDD82 pKa = 4.17 DD83 pKa = 3.89 EE84 pKa = 5.3 DD85 pKa = 5.17 SFMFPDD91 pKa = 4.56 PGAMHH96 pKa = 7.26 GAPAGSDD103 pKa = 2.99 EE104 pKa = 4.25 GQWLDD109 pKa = 3.46 SVLAEE114 pKa = 4.52 LQDD117 pKa = 5.35 DD118 pKa = 4.28 GDD120 pKa = 5.07 DD121 pKa = 4.44 DD122 pKa = 4.4 VDD124 pKa = 3.8 AAASPTSAVDD134 pKa = 3.57 EE135 pKa = 4.8 DD136 pKa = 5.02 DD137 pKa = 4.97 EE138 pKa = 4.7 PLSPLLSPMSSSDD151 pKa = 3.74 DD152 pKa = 3.69 LVSSSSFYY160 pKa = 10.72 YY161 pKa = 9.62 PPPIAIPYY169 pKa = 6.71 PVPYY173 pKa = 10.12 PPLQPTASEE182 pKa = 4.46 WYY184 pKa = 9.4 DD185 pKa = 3.4 VARR188 pKa = 11.84 SPVALLNASPFDD200 pKa = 4.09 DD201 pKa = 4.25 ALPYY205 pKa = 10.76 YY206 pKa = 10.38 YY207 pKa = 10.69 ADD209 pKa = 5.2 DD210 pKa = 4.61 LDD212 pKa = 4.48 EE213 pKa = 5.23 PPVPDD218 pKa = 5.6 AIEE221 pKa = 4.42 DD222 pKa = 3.94 TSDD225 pKa = 4.1 DD226 pKa = 3.89 EE227 pKa = 5.66 SDD229 pKa = 3.71 APSTPFYY236 pKa = 10.87 RR237 pKa = 11.84 STTSLDD243 pKa = 3.58 PASVPLPPEE252 pKa = 3.86 QHH254 pKa = 5.38 PHH256 pKa = 6.32 VYY258 pKa = 9.64 IDD260 pKa = 3.59 SDD262 pKa = 3.56 DD263 pKa = 4.08 SYY265 pKa = 11.56 FYY267 pKa = 10.55 PFEE270 pKa = 5.35 LDD272 pKa = 3.53 PLPSRR277 pKa = 11.84 SHH279 pKa = 6.88 ADD281 pKa = 3.17 GAGTARR287 pKa = 11.84 VFGTSLYY294 pKa = 9.89 QEE296 pKa = 4.75 CC297 pKa = 4.63
Molecular weight: 31.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.745
IPC_protein 3.783
Toseland 3.554
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.452
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.177
Thurlkill 3.605
EMBOSS 3.745
Sillero 3.91
Patrickios 0.82
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.795
Protein with the highest isoelectric point:
>tr|M2QVN3|M2QVN3_CERS8 Flavodoxin-like domain-containing protein OS=Ceriporiopsis subvermispora (strain B) OX=914234 GN=CERSUDRAFT_145890 PE=4 SV=1
RR1 pKa = 7.02 CTLSRR6 pKa = 11.84 TTRR9 pKa = 11.84 ARR11 pKa = 11.84 SSARR15 pKa = 11.84 SACSSTRR22 pKa = 11.84 RR23 pKa = 11.84 PSSAGPAPSASWWSPRR39 pKa = 11.84 ARR41 pKa = 11.84 RR42 pKa = 11.84 SRR44 pKa = 11.84 TSPSSPSRR52 pKa = 11.84 ASSPSPPPRR61 pKa = 11.84 RR62 pKa = 11.84 SSRR65 pKa = 11.84 SSS67 pKa = 2.88
Molecular weight: 7.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.383
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.842
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12077
1
12078
5150447
37
5567
426.4
47.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.234 ± 0.021
1.319 ± 0.01
5.628 ± 0.015
5.868 ± 0.022
3.569 ± 0.013
6.529 ± 0.023
2.602 ± 0.01
4.697 ± 0.017
4.045 ± 0.023
9.277 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.128 ± 0.007
3.112 ± 0.012
6.58 ± 0.029
3.759 ± 0.013
6.608 ± 0.019
8.447 ± 0.029
6.0 ± 0.015
6.511 ± 0.016
1.479 ± 0.008
2.607 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here