Acholeplasma sp. CAG:878

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae; Acholeplasma; environmental samples

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1145 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5HS54|R5HS54_9MOLU Type I phosphodiesterase/nucleotide pyrophosphatase OS=Acholeplasma sp. CAG:878 OX=1262686 GN=BN802_00217 PE=4 SV=1
MM1 pKa = 7.63SFPTTWSVPSGIVEE15 pKa = 4.51KK16 pKa = 11.13YY17 pKa = 10.72SEE19 pKa = 4.45LCSNDD24 pKa = 2.86EE25 pKa = 4.66FYY27 pKa = 10.69WEE29 pKa = 4.74YY30 pKa = 11.2EE31 pKa = 3.99DD32 pKa = 4.79EE33 pKa = 5.82DD34 pKa = 4.59YY35 pKa = 11.49DD36 pKa = 3.71GTHH39 pKa = 7.96IIRR42 pKa = 11.84KK43 pKa = 9.3INGEE47 pKa = 4.51LIDD50 pKa = 4.52TIAPNNLKK58 pKa = 10.71EE59 pKa = 4.06EE60 pKa = 4.93DD61 pKa = 3.98YY62 pKa = 11.63LDD64 pKa = 3.59VV65 pKa = 4.32

Molecular weight: 7.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5HDF0|R5HDF0_9MOLU Calcium-translocating P-type ATPase PMCA-type OS=Acholeplasma sp. CAG:878 OX=1262686 GN=BN802_00319 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.37RR12 pKa = 11.84SKK14 pKa = 11.19KK15 pKa = 8.94MGFLARR21 pKa = 11.84MQTKK25 pKa = 9.79VINSRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84KK33 pKa = 7.9GRR35 pKa = 11.84KK36 pKa = 8.73VLSHH40 pKa = 6.79

Molecular weight: 4.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1145

0

1145

330793

30

1985

288.9

33.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.103 ± 0.063

1.127 ± 0.029

5.805 ± 0.053

7.199 ± 0.081

4.366 ± 0.064

5.323 ± 0.067

1.237 ± 0.024

10.319 ± 0.114

10.038 ± 0.062

9.449 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.263 ± 0.031

7.786 ± 0.079

2.465 ± 0.038

2.155 ± 0.036

3.019 ± 0.044

6.096 ± 0.057

5.398 ± 0.073

6.053 ± 0.056

0.573 ± 0.022

5.228 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski