Blastomonas sp. AAP25

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Blastomonas; unclassified Blastomonas

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3685 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N1BGC7|A0A0N1BGC7_9SPHN Glyco_hydro_19_cat domain-containing protein OS=Blastomonas sp. AAP25 OX=1523416 GN=IP68_12500 PE=4 SV=1
MM1 pKa = 8.04RR2 pKa = 11.84STSDD6 pKa = 3.37SVSVRR11 pKa = 11.84LYY13 pKa = 11.27LLDD16 pKa = 4.34GDD18 pKa = 4.5VEE20 pKa = 4.62GGATSSLFYY29 pKa = 11.13GPLAEE34 pKa = 4.85ALHH37 pKa = 6.59IAAQQSAEE45 pKa = 4.17VQEE48 pKa = 4.42GLYY51 pKa = 10.19IATDD55 pKa = 3.63NDD57 pKa = 3.3VVAYY61 pKa = 10.59LDD63 pKa = 4.81LIEE66 pKa = 4.32EE67 pKa = 4.43

Molecular weight: 7.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N1BE70|A0A0N1BE70_9SPHN Peptidase U35 OS=Blastomonas sp. AAP25 OX=1523416 GN=IP68_18580 PE=4 SV=1
MM1 pKa = 6.88FACVKK6 pKa = 9.75LASARR11 pKa = 11.84GVHH14 pKa = 5.14VLEE17 pKa = 4.41SVFYY21 pKa = 8.41RR22 pKa = 11.84QLMGVLLMVPVALAGPGLAALKK44 pKa = 7.5TQRR47 pKa = 11.84PWTHH51 pKa = 6.54ASRR54 pKa = 11.84MVVGMVAMTLNFLSFALLPIAEE76 pKa = 4.15ATAIGFMVPVVATLLSIFLLRR97 pKa = 11.84EE98 pKa = 3.9SVGVHH103 pKa = 4.39RR104 pKa = 11.84WGAIAVGLTGVLVVIQPGDD123 pKa = 3.41GHH125 pKa = 6.6IPPTGALVAMCGVAMTAWVSILIRR149 pKa = 11.84KK150 pKa = 9.2LGATEE155 pKa = 4.43SPVTTMFWFSVSSMVPLGIAMLFVGQAHH183 pKa = 7.56DD184 pKa = 3.65AQTWGIIALMGLFGAAAQLGITWSLRR210 pKa = 11.84LAPVSVVLPMDD221 pKa = 3.64YY222 pKa = 11.13SSLIWAALIGWLIWQNWPVPATWIGAPLIIGSGLYY257 pKa = 9.65IAWRR261 pKa = 11.84EE262 pKa = 3.74NRR264 pKa = 11.84LARR267 pKa = 11.84LRR269 pKa = 11.84KK270 pKa = 9.14RR271 pKa = 3.46

Molecular weight: 29.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3685

0

3685

1174460

37

4451

318.7

34.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.143 ± 0.058

0.826 ± 0.011

6.08 ± 0.03

5.261 ± 0.032

3.658 ± 0.025

8.916 ± 0.051

1.982 ± 0.019

5.278 ± 0.026

2.98 ± 0.035

9.721 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.626 ± 0.025

2.667 ± 0.027

5.256 ± 0.034

3.358 ± 0.023

6.966 ± 0.038

5.378 ± 0.033

5.275 ± 0.03

6.982 ± 0.028

1.429 ± 0.016

2.218 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski