Bat circovirus ZS/China/2011
Average proteome isoelectric point is 7.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G1D7G1|G1D7G1_9CIRC Putative capsid protein OS=Bat circovirus ZS/China/2011 OX=1072162 PE=4 SV=1
MM1 pKa = 7.84 PCLQARR7 pKa = 11.84 YY8 pKa = 8.35 WLLTIPYY15 pKa = 9.12 EE16 pKa = 4.19 HH17 pKa = 6.85 FTPYY21 pKa = 10.66 LPPNCAYY28 pKa = 9.81 IKK30 pKa = 10.65 GQLEE34 pKa = 4.11 QGSNTSYY41 pKa = 10.88 LHH43 pKa = 5.57 WQLVVYY49 pKa = 8.08 FSQKK53 pKa = 10.32 KK54 pKa = 8.36 SLNYY58 pKa = 10.01 VKK60 pKa = 10.7 LIFGDD65 pKa = 5.23 GIHH68 pKa = 6.8 CEE70 pKa = 3.95 PSKK73 pKa = 11.22 SKK75 pKa = 10.7 AAEE78 pKa = 3.93 EE79 pKa = 4.42 YY80 pKa = 9.67 VWKK83 pKa = 9.97 EE84 pKa = 3.73 DD85 pKa = 3.29 TSIPGTQFEE94 pKa = 4.78 LGKK97 pKa = 10.62 NSIKK101 pKa = 10.19 RR102 pKa = 11.84 DD103 pKa = 3.54 STKK106 pKa = 11.07 DD107 pKa = 2.47 WDD109 pKa = 5.15 LIVKK113 pKa = 9.16 HH114 pKa = 6.14 ARR116 pKa = 11.84 EE117 pKa = 4.3 GDD119 pKa = 3.63 FASIPGDD126 pKa = 3.43 VLVRR130 pKa = 11.84 CYY132 pKa = 11.49 GNLKK136 pKa = 9.9 KK137 pKa = 10.4 IRR139 pKa = 11.84 VDD141 pKa = 3.35 SLQPEE146 pKa = 4.59 SIVRR150 pKa = 11.84 EE151 pKa = 3.69 IYY153 pKa = 10.28 VYY155 pKa = 10.55 YY156 pKa = 10.81 GRR158 pKa = 11.84 TGAGKK163 pKa = 9.71 SRR165 pKa = 11.84 RR166 pKa = 11.84 AWEE169 pKa = 3.97 EE170 pKa = 3.77 ATLSAYY176 pKa = 9.81 PKK178 pKa = 10.66 DD179 pKa = 3.98 PNTKK183 pKa = 9.62 FWDD186 pKa = 4.78 GYY188 pKa = 10.96 AGQEE192 pKa = 4.02 CVVIDD197 pKa = 4.08 EE198 pKa = 4.38 FRR200 pKa = 11.84 GAISISHH207 pKa = 7.22 LLRR210 pKa = 11.84 WLDD213 pKa = 3.65 RR214 pKa = 11.84 YY215 pKa = 9.87 PVIVEE220 pKa = 4.16 IKK222 pKa = 10.51 GSSCVFKK229 pKa = 10.9 AKK231 pKa = 10.39 KK232 pKa = 9.58 IWITSNLSPDD242 pKa = 3.25 DD243 pKa = 4.05 WYY245 pKa = 10.61 PDD247 pKa = 3.23 LDD249 pKa = 4.92 AEE251 pKa = 4.68 TKK253 pKa = 9.62 SALRR257 pKa = 11.84 RR258 pKa = 11.84 RR259 pKa = 11.84 FTEE262 pKa = 4.13 VVHH265 pKa = 6.78 FNN267 pKa = 3.4
Molecular weight: 30.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.162
IPC2_protein 7.497
IPC_protein 7.439
Toseland 7.102
ProMoST 7.907
Dawson 8.112
Bjellqvist 8.39
Wikipedia 8.039
Rodwell 8.126
Grimsley 7.146
Solomon 8.214
Lehninger 8.229
Nozaki 8.536
DTASelect 8.185
Thurlkill 8.229
EMBOSS 8.331
Sillero 8.536
Patrickios 4.101
IPC_peptide 8.2
IPC2_peptide 7.395
IPC2.peptide.svr19 7.605
Protein with the highest isoelectric point:
>tr|G1D7G1|G1D7G1_9CIRC Putative capsid protein OS=Bat circovirus ZS/China/2011 OX=1072162 PE=4 SV=1
MM1 pKa = 6.47 TQVIKK6 pKa = 11.17 GMMALTLYY14 pKa = 10.38 GINGTNDD21 pKa = 2.78 GNTVQGCRR29 pKa = 11.84 DD30 pKa = 2.97 IYY32 pKa = 10.78 RR33 pKa = 11.84 IFKK36 pKa = 10.32 NDD38 pKa = 3.71 PDD40 pKa = 3.92 VQQTGVAPGNEE51 pKa = 4.3 PYY53 pKa = 10.31 NGKK56 pKa = 8.88 LQFGSGVLDD65 pKa = 3.23 VTIRR69 pKa = 11.84 NLSDD73 pKa = 3.67 SIEE76 pKa = 4.44 AEE78 pKa = 3.74 VDD80 pKa = 3.11 IYY82 pKa = 10.94 TGWFRR87 pKa = 11.84 KK88 pKa = 9.86 SQDD91 pKa = 2.94 NTAVLGSRR99 pKa = 11.84 NPVTDD104 pKa = 4.33 FSNPSAITPIAPGNTGTQIYY124 pKa = 10.33 EE125 pKa = 3.95 RR126 pKa = 11.84 GTTLFDD132 pKa = 3.25 GTTALSKK139 pKa = 9.88 TGFHH143 pKa = 6.47 IHH145 pKa = 7.04 RR146 pKa = 11.84 KK147 pKa = 6.68 TKK149 pKa = 10.57 SILAPTQSMFLQHH162 pKa = 7.26 RR163 pKa = 11.84 DD164 pKa = 3.8 PKK166 pKa = 11.12 NHH168 pKa = 6.34 MIDD171 pKa = 3.14 WVRR174 pKa = 11.84 DD175 pKa = 3.62 GVVGYY180 pKa = 10.23 AKK182 pKa = 10.54 KK183 pKa = 10.63 GLTYY187 pKa = 11.13 GMIIVWKK194 pKa = 9.06 PSVLASSEE202 pKa = 4.43 AIVPLAVGTTRR213 pKa = 11.84 KK214 pKa = 8.13 YY215 pKa = 8.63 TYY217 pKa = 11.12 AVVEE221 pKa = 4.44 DD222 pKa = 3.91 NLDD225 pKa = 3.77 RR226 pKa = 11.84 VCKK229 pKa = 10.5 NPPLL233 pKa = 4.4
Molecular weight: 25.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.973
IPC2_protein 8.053
IPC_protein 8.112
Toseland 8.463
ProMoST 8.448
Dawson 8.799
Bjellqvist 8.785
Wikipedia 8.887
Rodwell 8.858
Grimsley 8.463
Solomon 9.019
Lehninger 8.99
Nozaki 8.843
DTASelect 8.682
Thurlkill 8.741
EMBOSS 8.975
Sillero 8.96
Patrickios 4.444
IPC_peptide 9.004
IPC2_peptide 7.585
IPC2.peptide.svr19 7.622
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
500
233
267
250.0
28.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.6 ± 0.013
1.6 ± 0.453
6.2 ± 0.145
4.6 ± 1.236
3.4 ± 0.241
7.6 ± 1.124
2.0 ± 0.173
6.8 ± 0.041
6.8 ± 0.745
7.2 ± 0.465
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.4 ± 0.717
4.4 ± 0.982
5.4 ± 0.109
3.4 ± 0.282
5.2 ± 0.292
6.8 ± 0.745
7.0 ± 1.752
7.2 ± 0.582
2.4 ± 0.679
5.0 ± 0.694
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here