Aphanizomenon phage vB_AphaS-CL131

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 149 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4PAY9|A0A2H4PAY9_9CAUD Uncharacterized protein OS=Aphanizomenon phage vB_AphaS-CL131 OX=2047766 GN=Cl131_gp110 PE=4 SV=1
MM1 pKa = 6.82VTINPNDD8 pKa = 3.67FQSSFGYY15 pKa = 10.7LSTCHH20 pKa = 6.56EE21 pKa = 4.19YY22 pKa = 10.72KK23 pKa = 10.23GWKK26 pKa = 7.84IKK28 pKa = 10.81YY29 pKa = 6.98YY30 pKa = 10.54APNGGILAVDD40 pKa = 4.44FGKK43 pKa = 10.43DD44 pKa = 3.25EE45 pKa = 4.38YY46 pKa = 11.06FSEE49 pKa = 4.37ATSIDD54 pKa = 3.48PCEE57 pKa = 4.21DD58 pKa = 3.38TEE60 pKa = 5.05IFLDD64 pKa = 4.11FAKK67 pKa = 10.6EE68 pKa = 4.04VIDD71 pKa = 3.79EE72 pKa = 4.47FEE74 pKa = 4.9ANLVAAA80 pKa = 5.5

Molecular weight: 9.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4PB05|A0A2H4PB05_9CAUD Uncharacterized protein OS=Aphanizomenon phage vB_AphaS-CL131 OX=2047766 GN=Cl131_gp139 PE=4 SV=1
MM1 pKa = 7.31NGIISVNDD9 pKa = 3.49FRR11 pKa = 11.84KK12 pKa = 10.05SWITWRR18 pKa = 11.84THH20 pKa = 3.62WKK22 pKa = 9.49RR23 pKa = 11.84NKK25 pKa = 10.47LITRR29 pKa = 11.84SLSIGDD35 pKa = 3.65RR36 pKa = 11.84VTHH39 pKa = 5.68QRR41 pKa = 11.84FSTLLIAMLLRR52 pKa = 11.84YY53 pKa = 9.49LLEE56 pKa = 4.21YY57 pKa = 10.66LDD59 pKa = 4.78IYY61 pKa = 11.16

Molecular weight: 7.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

149

0

149

34343

24

2131

230.5

25.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.658 ± 0.427

1.13 ± 0.107

5.483 ± 0.225

6.814 ± 0.284

3.925 ± 0.143

6.211 ± 0.195

1.575 ± 0.143

7.16 ± 0.158

7.495 ± 0.413

8.546 ± 0.303

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.904 ± 0.121

5.233 ± 0.148

3.983 ± 0.178

4.583 ± 0.31

4.647 ± 0.179

7.358 ± 0.222

6.019 ± 0.411

5.596 ± 0.138

1.339 ± 0.138

3.34 ± 0.198
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski