Haloferax tailed virus 1
Average proteome isoelectric point is 5.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A410N6Q2|A0A410N6Q2_9VIRU Portal protein OS=Haloferax tailed virus 1 OX=2507575 GN=HFTV1-gp13 PE=4 SV=1
MM1 pKa = 7.59 SDD3 pKa = 3.76 EE4 pKa = 4.36 SGGWLTDD11 pKa = 3.4 AVSDD15 pKa = 4.73 GIQTVRR21 pKa = 11.84 EE22 pKa = 4.19 TVSFLGVEE30 pKa = 4.31 RR31 pKa = 11.84 EE32 pKa = 3.99 KK33 pKa = 10.97 HH34 pKa = 5.54 VCDD37 pKa = 3.83 HH38 pKa = 6.78 CNVACEE44 pKa = 4.05 EE45 pKa = 4.33 TFVFDD50 pKa = 3.52 VRR52 pKa = 11.84 QAAFDD57 pKa = 4.55 DD58 pKa = 4.75 GQTPAWEE65 pKa = 4.5 CPEE68 pKa = 4.61 CEE70 pKa = 3.52 QTYY73 pKa = 9.91 YY74 pKa = 11.02 RR75 pKa = 11.84 EE76 pKa = 4.34 TGDD79 pKa = 3.71 EE80 pKa = 4.27 SHH82 pKa = 7.13 TMDD85 pKa = 5.02 LYY87 pKa = 11.45 DD88 pKa = 3.51 RR89 pKa = 4.97
Molecular weight: 10.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.834
IPC2_protein 4.088
IPC_protein 4.012
Toseland 3.834
ProMoST 4.151
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.897
Rodwell 3.859
Grimsley 3.745
Solomon 3.973
Lehninger 3.935
Nozaki 4.101
DTASelect 4.279
Thurlkill 3.872
EMBOSS 3.91
Sillero 4.139
Patrickios 0.998
IPC_peptide 3.973
IPC2_peptide 4.113
IPC2.peptide.svr19 4.001
Protein with the highest isoelectric point:
>tr|A0A410N6Y0|A0A410N6Y0_9VIRU Uncharacterized protein OS=Haloferax tailed virus 1 OX=2507575 GN=HFTV1-gp57 PE=4 SV=1
MM1 pKa = 7.37 GRR3 pKa = 11.84 GNRR6 pKa = 11.84 TRR8 pKa = 11.84 QFITCRR14 pKa = 11.84 EE15 pKa = 4.13 CGRR18 pKa = 11.84 RR19 pKa = 11.84 AIVIDD24 pKa = 3.58 QHH26 pKa = 6.77 ALPPRR31 pKa = 11.84 YY32 pKa = 9.72 CGACQIKK39 pKa = 10.26 RR40 pKa = 11.84 EE41 pKa = 4.11 ARR43 pKa = 11.84 EE44 pKa = 3.8
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.298
IPC2_protein 9.18
IPC_protein 9.97
Toseland 9.999
ProMoST 9.882
Dawson 10.218
Bjellqvist 10.131
Wikipedia 10.452
Rodwell 10.204
Grimsley 10.277
Solomon 10.394
Lehninger 10.365
Nozaki 10.335
DTASelect 10.028
Thurlkill 10.116
EMBOSS 10.438
Sillero 10.262
Patrickios 10.204
IPC_peptide 10.379
IPC2_peptide 9.911
IPC2.peptide.svr19 8.123
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
68
0
68
11338
33
954
166.7
18.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.903 ± 0.244
1.094 ± 0.25
9.314 ± 0.241
8.89 ± 0.45
3.29 ± 0.192
7.462 ± 0.309
1.94 ± 0.188
4.489 ± 0.259
3.819 ± 0.293
6.756 ± 0.246
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.011 ± 0.185
3.872 ± 0.229
4.225 ± 0.22
3.466 ± 0.192
5.654 ± 0.264
7.047 ± 0.33
7.162 ± 0.383
7.294 ± 0.286
1.543 ± 0.145
2.769 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here