Mycobacterium phage Purky

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pclasvirinae; unclassified Pclasvirinae

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514TWQ3|A0A514TWQ3_9CAUD Uncharacterized protein OS=Mycobacterium phage Purky OX=2593351 GN=22 PE=4 SV=1
MM1 pKa = 7.35LTTGGIQLSNPTNAEE16 pKa = 3.81GYY18 pKa = 9.39EE19 pKa = 4.2FAFHH23 pKa = 6.83FSVKK27 pKa = 9.61GVKK30 pKa = 9.89LADD33 pKa = 4.48PGDD36 pKa = 4.09ADD38 pKa = 3.14TWYY41 pKa = 10.23PNIQYY46 pKa = 10.62GLRR49 pKa = 11.84GAGDD53 pKa = 4.03EE54 pKa = 4.23PPPLIVQAYY63 pKa = 9.66LMNAALNPDD72 pKa = 3.87PEE74 pKa = 4.5SVRR77 pKa = 11.84FSILYY82 pKa = 7.95TEE84 pKa = 4.42PVTWTEE90 pKa = 3.91VSPEE94 pKa = 4.09EE95 pKa = 4.29VDD97 pKa = 3.63ALPGSVEE104 pKa = 4.33GYY106 pKa = 10.25SFAIMGDD113 pKa = 3.55SADD116 pKa = 3.63EE117 pKa = 4.22NGAQFRR123 pKa = 11.84LGLVGDD129 pKa = 4.31GAYY132 pKa = 10.36GLEE135 pKa = 4.12VSLFTLPIYY144 pKa = 10.72LDD146 pKa = 3.69SQDD149 pKa = 3.95EE150 pKa = 4.21EE151 pKa = 4.94GAQLRR156 pKa = 11.84NVMLAHH162 pKa = 7.59APRR165 pKa = 11.84INWWAWEE172 pKa = 3.87MDD174 pKa = 3.32AA175 pKa = 6.11

Molecular weight: 19.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514TWR6|A0A514TWR6_9CAUD Helix-turn-helix DNA binding protein OS=Mycobacterium phage Purky OX=2593351 GN=31 PE=4 SV=1
MM1 pKa = 7.47SIAMPTRR8 pKa = 11.84RR9 pKa = 11.84SPWMNDD15 pKa = 2.75RR16 pKa = 11.84AGLLVRR22 pKa = 11.84LLQEE26 pKa = 3.77RR27 pKa = 11.84HH28 pKa = 5.42GLQLTEE34 pKa = 3.87SAARR38 pKa = 11.84DD39 pKa = 3.93TISDD43 pKa = 3.46HH44 pKa = 5.92VDD46 pKa = 3.02RR47 pKa = 11.84VAEE50 pKa = 3.98VMRR53 pKa = 11.84IGRR56 pKa = 11.84QSAKK60 pKa = 10.26PYY62 pKa = 9.23VTDD65 pKa = 3.72SVIGEE70 pKa = 4.05MADD73 pKa = 3.95RR74 pKa = 11.84IAAAVQAHH82 pKa = 7.11RR83 pKa = 11.84DD84 pKa = 3.43RR85 pKa = 11.84GGRR88 pKa = 11.84PHH90 pKa = 6.79LRR92 pKa = 11.84VVEE95 pKa = 4.17

Molecular weight: 10.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

16440

44

1226

195.7

21.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.995 ± 0.565

1.095 ± 0.138

6.892 ± 0.214

6.022 ± 0.311

2.737 ± 0.182

8.625 ± 0.584

1.88 ± 0.175

4.58 ± 0.198

3.175 ± 0.209

7.871 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.074 ± 0.125

2.786 ± 0.127

6.661 ± 0.234

3.802 ± 0.215

7.153 ± 0.389

4.982 ± 0.246

6.314 ± 0.22

6.995 ± 0.267

2.074 ± 0.178

2.287 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski