Microbacterium phage Phinky

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Squashvirus; unclassified Squashvirus

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 106 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8LEA1|A0A7G8LEA1_9CAUD Uncharacterized protein OS=Microbacterium phage Phinky OX=2762418 GN=82 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.55KK3 pKa = 10.56FIACAATAAIATLGLTALAVPASATTLDD31 pKa = 3.55SFGICVPTEE40 pKa = 3.97DD41 pKa = 3.36TTQTIVHH48 pKa = 6.75PAVGTPTIEE57 pKa = 4.26VNNPDD62 pKa = 3.93YY63 pKa = 11.36VPAVPGTPGVPAIGEE78 pKa = 4.08PTIEE82 pKa = 4.47IPNPAYY88 pKa = 10.81VPGVPAVPGTPAVGEE103 pKa = 4.12EE104 pKa = 4.51TILVPNPGYY113 pKa = 10.78VPAVPAVPGTPAVGEE128 pKa = 4.07PTIEE132 pKa = 4.25VPNPDD137 pKa = 3.92YY138 pKa = 10.96VAPTYY143 pKa = 10.65TPGFWQDD150 pKa = 3.02VPAVGTPTIIVTKK163 pKa = 9.43PNPDD167 pKa = 3.41YY168 pKa = 11.12VPAVAEE174 pKa = 4.06IPEE177 pKa = 4.26VSHH180 pKa = 6.44TDD182 pKa = 3.65YY183 pKa = 10.77VAKK186 pKa = 10.29RR187 pKa = 11.84YY188 pKa = 10.6VIDD191 pKa = 3.51QAAQPEE197 pKa = 4.59VTKK200 pKa = 8.5TQWRR204 pKa = 11.84YY205 pKa = 9.14VKK207 pKa = 10.52NGGYY211 pKa = 10.6GEE213 pKa = 4.38VWLDD217 pKa = 3.35SNAEE221 pKa = 4.09HH222 pKa = 6.74KK223 pKa = 11.1VKK225 pKa = 10.73INGYY229 pKa = 8.05WYY231 pKa = 10.19EE232 pKa = 4.13RR233 pKa = 11.84TNKK236 pKa = 8.78TKK238 pKa = 10.19TVVVTPAIPEE248 pKa = 4.13RR249 pKa = 11.84GHH251 pKa = 6.56WEE253 pKa = 3.64QQSFHH258 pKa = 7.31EE259 pKa = 4.39YY260 pKa = 9.92PGAGWTILSEE270 pKa = 4.56SKK272 pKa = 10.61HH273 pKa = 4.16VTQEE277 pKa = 4.03YY278 pKa = 10.23VPGSPAQGSEE288 pKa = 4.17TIDD291 pKa = 3.42VEE293 pKa = 4.45EE294 pKa = 4.84ANPDD298 pKa = 3.61YY299 pKa = 11.47VPATTVWVPPVYY311 pKa = 10.31TPAQGTKK318 pKa = 9.78TIAKK322 pKa = 9.13DD323 pKa = 3.44NPDD326 pKa = 3.65YY327 pKa = 11.51VPATEE332 pKa = 5.27EE333 pKa = 4.05IPGTPAVGEE342 pKa = 4.36PEE344 pKa = 4.75LEE346 pKa = 4.46VPNPAYY352 pKa = 9.83IPATEE357 pKa = 4.25GTPEE361 pKa = 3.67IPAVGEE367 pKa = 3.85EE368 pKa = 4.86TITIDD373 pKa = 3.22NPAYY377 pKa = 9.65IPAVPAVDD385 pKa = 5.15EE386 pKa = 4.47IPAVGEE392 pKa = 3.67PTIEE396 pKa = 4.14VEE398 pKa = 4.2NPDD401 pKa = 4.4YY402 pKa = 10.92IPEE405 pKa = 3.99RR406 pKa = 11.84TEE408 pKa = 3.99IITIPGTEE416 pKa = 4.58CPPVIPEE423 pKa = 4.07EE424 pKa = 4.31PEE426 pKa = 3.9EE427 pKa = 4.12PDD429 pKa = 3.32YY430 pKa = 11.28TLLDD434 pKa = 3.6GSISVDD440 pKa = 3.47CVAGTVTFRR449 pKa = 11.84GSNGTDD455 pKa = 3.04EE456 pKa = 4.51TVEE459 pKa = 4.0FATGFDD465 pKa = 3.7SDD467 pKa = 3.72GDD469 pKa = 4.13GAVDD473 pKa = 4.58FGDD476 pKa = 4.6GFIAPADD483 pKa = 3.61GAGQFVYY490 pKa = 10.1TFAEE494 pKa = 4.15LAGNEE499 pKa = 4.4SLTAHH504 pKa = 6.6LFIWGEE510 pKa = 4.03GWDD513 pKa = 3.88VAHH516 pKa = 6.47TVATSDD522 pKa = 3.19EE523 pKa = 4.32FSPVVCEE530 pKa = 4.2TPTEE534 pKa = 4.28PEE536 pKa = 4.01EE537 pKa = 4.1PTTPEE542 pKa = 3.86EE543 pKa = 4.1PTTPEE548 pKa = 3.86EE549 pKa = 4.1PTTPEE554 pKa = 3.98EE555 pKa = 4.03PTVPEE560 pKa = 4.53EE561 pKa = 4.08PTVPTTPVTPAEE573 pKa = 4.04PVAPVVNPAPAAPAAGAARR592 pKa = 11.84AAKK595 pKa = 10.38APVAAEE601 pKa = 4.32ADD603 pKa = 3.81TLAQTGADD611 pKa = 3.49VAFGAVGAAGVALLAGAGLLLHH633 pKa = 6.94RR634 pKa = 11.84RR635 pKa = 11.84RR636 pKa = 11.84HH637 pKa = 4.64TSS639 pKa = 2.67

Molecular weight: 66.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8LE38|A0A7G8LE38_9CAUD Helix-turn-helix DNA binding domain protein OS=Microbacterium phage Phinky OX=2762418 GN=18 PE=4 SV=1
MM1 pKa = 7.88AKK3 pKa = 9.46RR4 pKa = 11.84DD5 pKa = 3.69RR6 pKa = 11.84PRR8 pKa = 11.84YY9 pKa = 8.45IPPRR13 pKa = 11.84LGQRR17 pKa = 11.84DD18 pKa = 3.89FARR21 pKa = 11.84TAMVPRR27 pKa = 11.84WFTRR31 pKa = 11.84EE32 pKa = 3.31AVAAFRR38 pKa = 11.84LTPYY42 pKa = 10.58DD43 pKa = 3.69FAAFTVIADD52 pKa = 3.75NLDD55 pKa = 3.25SHH57 pKa = 6.83GISTTAMTLIATRR70 pKa = 11.84GGMSEE75 pKa = 4.11GGAAKK80 pKa = 10.13AVNRR84 pKa = 11.84LIAVDD89 pKa = 5.7LIHH92 pKa = 6.88EE93 pKa = 4.98LDD95 pKa = 3.74PRR97 pKa = 11.84RR98 pKa = 11.84KK99 pKa = 8.95GHH101 pKa = 5.26IMRR104 pKa = 11.84YY105 pKa = 9.79GIAPDD110 pKa = 3.73IPWTEE115 pKa = 4.06TQRR118 pKa = 3.61

Molecular weight: 13.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

106

0

106

20058

30

1055

189.2

20.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.447 ± 0.363

0.853 ± 0.126

6.342 ± 0.204

7.005 ± 0.344

2.797 ± 0.131

8.575 ± 0.235

1.855 ± 0.172

4.906 ± 0.142

2.587 ± 0.203

7.394 ± 0.348

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.243 ± 0.138

2.991 ± 0.188

5.564 ± 0.384

3.724 ± 0.284

6.97 ± 0.347

5.788 ± 0.343

7.214 ± 0.474

7.344 ± 0.208

1.944 ± 0.157

2.458 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski