Maritimibacter alkaliphilus HTCC2654

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Maritimibacter; Maritimibacter alkaliphilus

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4692 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3VIA4|A3VIA4_9RHOB Uncharacterized protein OS=Maritimibacter alkaliphilus HTCC2654 OX=314271 GN=RB2654_01335 PE=4 SV=1
MM1 pKa = 7.28ATMNSGLGGPAGYY14 pKa = 10.35GEE16 pKa = 4.82NVFSSTSKK24 pKa = 7.72TTGNNDD30 pKa = 3.45DD31 pKa = 3.57GSVYY35 pKa = 10.74VDD37 pKa = 3.52TSSVFGDD44 pKa = 4.28GIDD47 pKa = 4.04FFGTTYY53 pKa = 10.75SGLYY57 pKa = 9.7VNSNGNITFGAANTAYY73 pKa = 8.51QTSNLAGTSSRR84 pKa = 11.84MIAPFWGDD92 pKa = 3.18VNINSGGEE100 pKa = 4.09IYY102 pKa = 10.12WDD104 pKa = 3.44LDD106 pKa = 3.37AAAGTVTITWDD117 pKa = 3.22GVAPYY122 pKa = 9.69NGSGANSFQIVLTDD136 pKa = 3.2IGGGDD141 pKa = 3.52FTVEE145 pKa = 5.05FIYY148 pKa = 10.85EE149 pKa = 4.47DD150 pKa = 3.71IQWTTGYY157 pKa = 10.09SQVAQAGITDD167 pKa = 4.1GGANDD172 pKa = 5.44FILPGSGNATTMSNYY187 pKa = 6.77EE188 pKa = 4.13TYY190 pKa = 11.07DD191 pKa = 3.25FGTNDD196 pKa = 3.69PNGTTDD202 pKa = 4.04FFFVNGQPVFGDD214 pKa = 3.78GVVEE218 pKa = 4.43GTSGADD224 pKa = 3.46TIDD227 pKa = 2.98AAYY230 pKa = 10.83YY231 pKa = 10.48GDD233 pKa = 4.63PDD235 pKa = 4.74GDD237 pKa = 4.95LIDD240 pKa = 5.87DD241 pKa = 4.48GDD243 pKa = 4.18GTGPAGNEE251 pKa = 3.53DD252 pKa = 4.88LIYY255 pKa = 10.95GFDD258 pKa = 3.64GDD260 pKa = 5.03DD261 pKa = 4.22NIFAGDD267 pKa = 4.13GDD269 pKa = 3.92DD270 pKa = 4.2TIYY273 pKa = 11.3GGDD276 pKa = 3.48GTDD279 pKa = 3.77NIFGEE284 pKa = 4.96DD285 pKa = 4.02GDD287 pKa = 4.04DD288 pKa = 4.37TIYY291 pKa = 11.25GDD293 pKa = 3.81NGPTGVTEE301 pKa = 4.24DD302 pKa = 4.86LNWSAQGADD311 pKa = 3.51GASIAGGFTQTTGTMDD327 pKa = 3.39VNVSFSADD335 pKa = 2.89GNNAVFEE342 pKa = 4.41VATAEE347 pKa = 4.22TNYY350 pKa = 10.37VAGGEE355 pKa = 4.02PFAPNSSAYY364 pKa = 10.65VYY366 pKa = 11.2GNGDD370 pKa = 3.7GPTGTVTIDD379 pKa = 3.53FAAVPGSGMSDD390 pKa = 3.13EE391 pKa = 4.45VQNVAFRR398 pKa = 11.84LNDD401 pKa = 3.16IDD403 pKa = 5.12SYY405 pKa = 11.48AGNHH409 pKa = 6.18IDD411 pKa = 3.51VLTVNAYY418 pKa = 10.12DD419 pKa = 3.86EE420 pKa = 5.09NGDD423 pKa = 3.86PVAVTLTAAGNDD435 pKa = 3.65SVSGNTVTAGGTLDD449 pKa = 4.22NPQDD453 pKa = 3.66AQGSVLVEE461 pKa = 3.59IDD463 pKa = 4.38GPVAWFEE470 pKa = 4.31VIYY473 pKa = 11.1VNALTGTHH481 pKa = 7.55GINITDD487 pKa = 3.53VSFDD491 pKa = 3.96TIPASGAADD500 pKa = 3.79NIFGGLGDD508 pKa = 3.96DD509 pKa = 4.61TIHH512 pKa = 7.14GEE514 pKa = 4.51AGDD517 pKa = 3.82DD518 pKa = 4.39VIWGDD523 pKa = 3.91EE524 pKa = 4.26GSDD527 pKa = 3.51TLFGGAGDD535 pKa = 3.8DD536 pKa = 4.47TIWAGAGDD544 pKa = 4.67TVTGGAGDD552 pKa = 3.94DD553 pKa = 4.4MIWLDD558 pKa = 3.88AANALGGPGAVIDD571 pKa = 4.94IIGSEE576 pKa = 4.14DD577 pKa = 3.96GEE579 pKa = 4.74TGGDD583 pKa = 3.47TLNFAGLIDD592 pKa = 4.22WGTISYY598 pKa = 9.89TDD600 pKa = 4.03AEE602 pKa = 4.71SGSATLSDD610 pKa = 3.62GTTVTFSNIEE620 pKa = 3.74NLIICFTGGTAIDD633 pKa = 4.41TPSGPCPVEE642 pKa = 3.57HH643 pKa = 6.81LRR645 pKa = 11.84PGDD648 pKa = 3.61LVLTRR653 pKa = 11.84DD654 pKa = 4.33RR655 pKa = 11.84GPQPIRR661 pKa = 11.84WIGRR665 pKa = 11.84RR666 pKa = 11.84TVPGTGTFAPIRR678 pKa = 11.84FEE680 pKa = 4.44RR681 pKa = 11.84GAFGNVAPLMVSPQHH696 pKa = 6.25RR697 pKa = 11.84MVYY700 pKa = 9.77SGSRR704 pKa = 11.84ANLYY708 pKa = 10.41FDD710 pKa = 3.84TPEE713 pKa = 3.73VMVPAKK719 pKa = 10.5HH720 pKa = 6.64LVDD723 pKa = 3.36QRR725 pKa = 11.84LIRR728 pKa = 11.84QVEE731 pKa = 4.63VPTVTYY737 pKa = 8.48FHH739 pKa = 7.12IMLDD743 pKa = 3.2RR744 pKa = 11.84HH745 pKa = 5.22EE746 pKa = 4.46VLWGNGAPSEE756 pKa = 4.4SFHH759 pKa = 6.78PGALGLDD766 pKa = 3.48ALAAEE771 pKa = 4.58ARR773 pKa = 11.84EE774 pKa = 4.05EE775 pKa = 4.13LLTLFPDD782 pKa = 3.97LRR784 pKa = 11.84ADD786 pKa = 3.45PGVYY790 pKa = 10.14GKK792 pKa = 7.46TARR795 pKa = 11.84RR796 pKa = 11.84VLKK799 pKa = 10.5RR800 pKa = 11.84FEE802 pKa = 4.18ARR804 pKa = 11.84LLAAA808 pKa = 5.49

Molecular weight: 83.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3VH24|A3VH24_9RHOB Uncharacterized protein OS=Maritimibacter alkaliphilus HTCC2654 OX=314271 GN=RB2654_14875 PE=4 SV=1
MM1 pKa = 7.4RR2 pKa = 11.84RR3 pKa = 11.84GRR5 pKa = 11.84IGSTGSAIFRR15 pKa = 11.84RR16 pKa = 11.84PGSTATIRR24 pKa = 11.84VAGSMRR30 pKa = 11.84KK31 pKa = 9.34PGG33 pKa = 3.3

Molecular weight: 3.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4692

0

4692

1363242

15

7434

290.5

31.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.122 ± 0.052

0.797 ± 0.012

6.541 ± 0.047

6.1 ± 0.037

3.794 ± 0.023

8.895 ± 0.041

2.035 ± 0.021

5.128 ± 0.028

3.186 ± 0.034

9.53 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.835 ± 0.021

2.574 ± 0.023

5.091 ± 0.031

2.86 ± 0.02

6.711 ± 0.049

4.935 ± 0.031

5.723 ± 0.039

7.523 ± 0.03

1.388 ± 0.017

2.233 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski