Yersinia phage fHe-Yen9-04
Average proteome isoelectric point is 5.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 531 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2C9CXZ4|A0A2C9CXZ4_9CAUD Uncharacterized protein OS=Yersinia phage fHe-Yen9-04 OX=2052742 GN=g369 PE=4 SV=1
MM1 pKa = 6.77 NQNYY5 pKa = 8.09 MMPYY9 pKa = 8.04 QGKK12 pKa = 7.75 TIAEE16 pKa = 4.1 EE17 pKa = 4.38 SIEE20 pKa = 3.95 LAQTFVDD27 pKa = 3.64 HH28 pKa = 7.16 FKK30 pKa = 11.38 DD31 pKa = 3.45 KK32 pKa = 11.17 FDD34 pKa = 4.66 FSPHH38 pKa = 5.55 IDD40 pKa = 4.08 KK41 pKa = 10.66 IAEE44 pKa = 4.6 DD45 pKa = 3.32 IEE47 pKa = 4.96 NYY49 pKa = 9.94 VFSGNWDD56 pKa = 3.18 HH57 pKa = 6.65 TIEE60 pKa = 4.24 VEE62 pKa = 4.04 VDD64 pKa = 3.48 GVPWIRR70 pKa = 11.84 VIQEE74 pKa = 3.97 DD75 pKa = 5.17 EE76 pKa = 4.3 YY77 pKa = 10.77 DD78 pKa = 3.3 QHH80 pKa = 7.47 MDD82 pKa = 3.54 EE83 pKa = 5.6 DD84 pKa = 4.64 DD85 pKa = 4.65 EE86 pKa = 4.5 EE87 pKa = 4.71 TPSNDD92 pKa = 3.02 DD93 pKa = 2.64 VDD95 pKa = 4.49 YY96 pKa = 11.32 VGGYY100 pKa = 10.44 YY101 pKa = 11.18 VMLVNN106 pKa = 5.49
Molecular weight: 12.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.808
IPC_protein 3.783
Toseland 3.579
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.49
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.101
Thurlkill 3.63
EMBOSS 3.719
Sillero 3.91
Patrickios 0.998
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.807
Protein with the highest isoelectric point:
>tr|A0A2C9CWZ0|A0A2C9CWZ0_9CAUD tRNAHis-5'-guanylyltransferase OS=Yersinia phage fHe-Yen9-04 OX=2052742 GN=g142 PE=4 SV=1
MM1 pKa = 6.54 WQCIKK6 pKa = 10.35 PRR8 pKa = 11.84 PLYY11 pKa = 9.38 RR12 pKa = 11.84 TAIEE16 pKa = 4.07 RR17 pKa = 11.84 NRR19 pKa = 11.84 TTRR22 pKa = 11.84 FHH24 pKa = 7.06 SFIEE28 pKa = 3.93 IRR30 pKa = 11.84 DD31 pKa = 3.77 MKK33 pKa = 10.73 LLRR36 pKa = 11.84 LSSSVFLPTRR46 pKa = 11.84 WGDD49 pKa = 3.53 EE50 pKa = 3.93 NTLTNLVSIINRR62 pKa = 11.84 STIQYY67 pKa = 9.11 YY68 pKa = 10.32 INN70 pKa = 3.58
Molecular weight: 8.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.78
IPC_protein 10.774
Toseland 10.511
ProMoST 10.394
Dawson 10.687
Bjellqvist 10.482
Wikipedia 10.965
Rodwell 10.716
Grimsley 10.774
Solomon 10.818
Lehninger 10.774
Nozaki 10.511
DTASelect 10.482
Thurlkill 10.555
EMBOSS 10.935
Sillero 10.613
Patrickios 10.496
IPC_peptide 10.818
IPC2_peptide 9.677
IPC2.peptide.svr19 8.316
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
531
0
531
107596
23
3339
202.6
23.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.843 ± 0.154
1.157 ± 0.054
6.501 ± 0.12
6.801 ± 0.173
4.576 ± 0.096
5.678 ± 0.168
1.905 ± 0.065
7.99 ± 0.137
6.696 ± 0.164
8.009 ± 0.09
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.687 ± 0.067
6.93 ± 0.123
3.051 ± 0.063
3.461 ± 0.082
3.708 ± 0.087
7.148 ± 0.138
6.483 ± 0.274
6.37 ± 0.102
1.128 ± 0.049
4.877 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here