Lactococcus phage 88605

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus; Lactococcus virus 88605

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A343JQM1|A0A343JQM1_9CAUD Putative terminase large subunit OS=Lactococcus phage 88605 OX=2029673 GN=88605_03 PE=4 SV=1
MM1 pKa = 6.54KK2 pKa = 10.05QYY4 pKa = 10.55WIIEE8 pKa = 4.05DD9 pKa = 3.85HH10 pKa = 7.12LDD12 pKa = 3.5GGLYY16 pKa = 10.03IMPEE20 pKa = 3.96DD21 pKa = 3.93TPEE24 pKa = 5.18DD25 pKa = 3.64EE26 pKa = 4.67LEE28 pKa = 4.2EE29 pKa = 4.84VEE31 pKa = 5.02DD32 pKa = 4.06TCDD35 pKa = 3.05EE36 pKa = 4.63CGDD39 pKa = 3.69FDD41 pKa = 4.95SVIGKK46 pKa = 8.97FSNWNQLKK54 pKa = 10.45KK55 pKa = 11.08EE56 pKa = 4.06MTDD59 pKa = 3.5DD60 pKa = 4.56EE61 pKa = 5.2GWCPYY66 pKa = 10.27SDD68 pKa = 4.92EE69 pKa = 4.17YY70 pKa = 11.5LKK72 pKa = 11.17SLFEE76 pKa = 4.13

Molecular weight: 9.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A343JQP7|A0A343JQP7_9CAUD Uncharacterized protein OS=Lactococcus phage 88605 OX=2029673 GN=88605_29 PE=4 SV=1
MM1 pKa = 7.46CKK3 pKa = 9.75KK4 pKa = 10.38RR5 pKa = 11.84KK6 pKa = 5.78YY7 pKa = 8.56TKK9 pKa = 9.78MGALYY14 pKa = 10.54SIANAQHH21 pKa = 5.85NKK23 pKa = 9.27KK24 pKa = 10.0KK25 pKa = 10.46AGKK28 pKa = 8.52IPVRR32 pKa = 11.84AYY34 pKa = 9.69HH35 pKa = 6.61CKK37 pKa = 8.93WCNLYY42 pKa = 10.43HH43 pKa = 7.32LSSQQRR49 pKa = 11.84LNIKK53 pKa = 9.21TGVIGG58 pKa = 3.97

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

9477

39

919

175.5

19.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.962 ± 0.504

0.707 ± 0.207

5.877 ± 0.273

7.281 ± 0.633

4.136 ± 0.243

6.384 ± 0.614

1.235 ± 0.169

7.091 ± 0.312

8.853 ± 0.589

8.6 ± 0.367

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.511 ± 0.151

6.859 ± 0.347

2.205 ± 0.217

3.619 ± 0.221

3.366 ± 0.213

6.584 ± 0.444

6.658 ± 0.387

6.31 ± 0.338

1.519 ± 0.148

4.242 ± 0.382
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski