Firmicutes bacterium CAG:176

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1505 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6DAK8|R6DAK8_9FIRM Phosphonates import ATP-binding protein PhnC OS=Firmicutes bacterium CAG:176 OX=1263007 GN=phnC PE=3 SV=1
MM1 pKa = 7.36IKK3 pKa = 10.24KK4 pKa = 10.01LAALTLALLMTLSMAACGSQKK25 pKa = 10.77DD26 pKa = 3.95DD27 pKa = 3.4QAGGNVTPGVTDD39 pKa = 3.89DD40 pKa = 4.77AVPDD44 pKa = 3.62MGTVSGGTYY53 pKa = 7.61TNRR56 pKa = 11.84FAGISCTLDD65 pKa = 3.46DD66 pKa = 3.17SWYY69 pKa = 10.44FYY71 pKa = 10.85TDD73 pKa = 3.34EE74 pKa = 5.86QIDD77 pKa = 3.94EE78 pKa = 4.48LNGFVRR84 pKa = 11.84EE85 pKa = 4.29STSDD89 pKa = 3.45EE90 pKa = 3.92EE91 pKa = 4.16LKK93 pKa = 11.08ARR95 pKa = 11.84LEE97 pKa = 4.46SSSSVQDD104 pKa = 3.12MYY106 pKa = 11.62AASTDD111 pKa = 3.13GLMTINVVFTNMGLLGGSTVTPQDD135 pKa = 3.41VAEE138 pKa = 4.51LSVEE142 pKa = 4.1QVPAALEE149 pKa = 4.41SYY151 pKa = 10.89GFTDD155 pKa = 3.19VTAQLTTVDD164 pKa = 4.44FAGQKK169 pKa = 9.43NVPAVAVSAMNGDD182 pKa = 3.15IATYY186 pKa = 10.35EE187 pKa = 4.11LLVCLKK193 pKa = 10.39AGNYY197 pKa = 9.12SFSVTLCSFTEE208 pKa = 4.3DD209 pKa = 3.22VTADD213 pKa = 3.31MAALFTAAQQ222 pKa = 3.46

Molecular weight: 23.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6DAI5|R6DAI5_9FIRM Putative D-lactate dehydrogenase OS=Firmicutes bacterium CAG:176 OX=1263007 GN=BN516_01191 PE=3 SV=1
MM1 pKa = 7.25FRR3 pKa = 11.84TYY5 pKa = 9.97QPKK8 pKa = 9.59KK9 pKa = 7.61RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.69EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 10.07RR21 pKa = 11.84MATRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.04GRR39 pKa = 11.84ARR41 pKa = 11.84LTHH44 pKa = 6.28

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1505

0

1505

449595

29

2946

298.7

32.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.573 ± 0.081

1.804 ± 0.028

5.816 ± 0.054

6.246 ± 0.071

3.531 ± 0.041

8.19 ± 0.066

1.893 ± 0.033

5.377 ± 0.058

4.534 ± 0.057

10.153 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.928 ± 0.03

2.955 ± 0.038

4.063 ± 0.042

3.307 ± 0.041

5.796 ± 0.061

4.974 ± 0.048

5.802 ± 0.057

7.699 ± 0.053

0.976 ± 0.025

3.378 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski