Amino acid dipepetide frequency for Streptomyces roseus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
24.042AlaAla: 24.042 ± 0.195
1.129AlaCys: 1.129 ± 0.022
8.308AlaAsp: 8.308 ± 0.077
9.178AlaGlu: 9.178 ± 0.105
3.688AlaPhe: 3.688 ± 0.05
14.449AlaGly: 14.449 ± 0.112
2.949AlaHis: 2.949 ± 0.039
3.413AlaIle: 3.413 ± 0.046
3.153AlaLys: 3.153 ± 0.051
15.142AlaLeu: 15.142 ± 0.134
2.635AlaMet: 2.635 ± 0.034
1.969AlaAsn: 1.969 ± 0.038
7.935AlaPro: 7.935 ± 0.081
4.115AlaGln: 4.115 ± 0.049
10.468AlaArg: 10.468 ± 0.09
5.965AlaSer: 5.965 ± 0.057
6.84AlaThr: 6.84 ± 0.058
12.543AlaVal: 12.543 ± 0.111
1.986AlaTrp: 1.986 ± 0.033
2.91AlaTyr: 2.91 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
1.172CysAla: 1.172 ± 0.027
0.084CysCys: 0.084 ± 0.008
0.449CysAsp: 0.449 ± 0.018
0.396CysGlu: 0.396 ± 0.014
0.225CysPhe: 0.225 ± 0.011
0.982CysGly: 0.982 ± 0.021
0.194CysHis: 0.194 ± 0.009
0.171CysIle: 0.171 ± 0.008
0.128CysLys: 0.128 ± 0.008
0.743CysLeu: 0.743 ± 0.019
0.131CysMet: 0.131 ± 0.008
0.132CysAsn: 0.132 ± 0.007
0.504CysPro: 0.504 ± 0.015
0.167CysGln: 0.167 ± 0.009
0.581CysArg: 0.581 ± 0.016
0.425CysSer: 0.425 ± 0.016
0.528CysThr: 0.528 ± 0.016
0.666CysVal: 0.666 ± 0.019
0.132CysTrp: 0.132 ± 0.008
0.143CysTyr: 0.143 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.515AspAla: 7.515 ± 0.064
0.441AspCys: 0.441 ± 0.013
2.838AspAsp: 2.838 ± 0.041
3.643AspGlu: 3.643 ± 0.046
1.575AspPhe: 1.575 ± 0.03
6.025AspGly: 6.025 ± 0.062
1.355AspHis: 1.355 ± 0.028
1.768AspIle: 1.768 ± 0.037
1.223AspLys: 1.223 ± 0.032
5.996AspLeu: 5.996 ± 0.051
0.803AspMet: 0.803 ± 0.02
0.899AspAsn: 0.899 ± 0.026
4.628AspPro: 4.628 ± 0.055
1.457AspGln: 1.457 ± 0.031
4.703AspArg: 4.703 ± 0.049
2.389AspSer: 2.389 ± 0.036
2.972AspThr: 2.972 ± 0.041
4.311AspVal: 4.311 ± 0.054
1.004AspTrp: 1.004 ± 0.022
1.075AspTyr: 1.075 ± 0.023
0.0AspXaa: 0.0 ± 0.0
Glu
7.825GluAla: 7.825 ± 0.09
0.366GluCys: 0.366 ± 0.013
2.85GluAsp: 2.85 ± 0.047
3.633GluGlu: 3.633 ± 0.05
1.532GluPhe: 1.532 ± 0.029
4.525GluGly: 4.525 ± 0.056
1.473GluHis: 1.473 ± 0.027
2.253GluIle: 2.253 ± 0.039
1.415GluLys: 1.415 ± 0.031
6.888GluLeu: 6.888 ± 0.076
0.895GluMet: 0.895 ± 0.023
1.054GluAsn: 1.054 ± 0.027
3.328GluPro: 3.328 ± 0.054
2.153GluGln: 2.153 ± 0.039
5.344GluArg: 5.344 ± 0.058
2.446GluSer: 2.446 ± 0.036
2.718GluThr: 2.718 ± 0.043
4.617GluVal: 4.617 ± 0.054
0.723GluTrp: 0.723 ± 0.021
1.136GluTyr: 1.136 ± 0.027
0.0GluXaa: 0.0 ± 0.0
Phe
3.808PheAla: 3.808 ± 0.051
0.269PheCys: 0.269 ± 0.011
1.875PheAsp: 1.875 ± 0.035
1.446PheGlu: 1.446 ± 0.028
0.846PhePhe: 0.846 ± 0.023
3.042PheGly: 3.042 ± 0.041
0.636PheHis: 0.636 ± 0.018
0.73PheIle: 0.73 ± 0.019
0.565PheLys: 0.565 ± 0.018
2.6PheLeu: 2.6 ± 0.037
0.418PheMet: 0.418 ± 0.014
0.564PheAsn: 0.564 ± 0.016
1.436PhePro: 1.436 ± 0.026
0.648PheGln: 0.648 ± 0.017
1.808PheArg: 1.808 ± 0.033
1.469PheSer: 1.469 ± 0.028
2.052PheThr: 2.052 ± 0.033
2.15PheVal: 2.15 ± 0.034
0.428PheTrp: 0.428 ± 0.015
0.595PheTyr: 0.595 ± 0.016
0.0PheXaa: 0.0 ± 0.0
Gly
12.128GlyAla: 12.128 ± 0.101
0.862GlyCys: 0.862 ± 0.02
4.862GlyAsp: 4.862 ± 0.053
5.227GlyGlu: 5.227 ± 0.048
2.961GlyPhe: 2.961 ± 0.041
9.371GlyGly: 9.371 ± 0.094
2.329GlyHis: 2.329 ± 0.041
3.622GlyIle: 3.622 ± 0.044
2.573GlyLys: 2.573 ± 0.045
9.622GlyLeu: 9.622 ± 0.076
2.003GlyMet: 2.003 ± 0.03
1.785GlyAsn: 1.785 ± 0.041
5.586GlyPro: 5.586 ± 0.053
2.75GlyGln: 2.75 ± 0.039
7.948GlyArg: 7.948 ± 0.062
5.603GlySer: 5.603 ± 0.06
6.111GlyThr: 6.111 ± 0.061
7.365GlyVal: 7.365 ± 0.072
1.733GlyTrp: 1.733 ± 0.029
2.311GlyTyr: 2.311 ± 0.034
0.0GlyXaa: 0.0 ± 0.0
His
2.746HisAla: 2.746 ± 0.042
0.193HisCys: 0.193 ± 0.01
1.255HisAsp: 1.255 ± 0.023
1.188HisGlu: 1.188 ± 0.024
0.618HisPhe: 0.618 ± 0.017
2.344HisGly: 2.344 ± 0.033
0.647HisHis: 0.647 ± 0.02
0.655HisIle: 0.655 ± 0.017
0.37HisLys: 0.37 ± 0.013
2.416HisLeu: 2.416 ± 0.041
0.339HisMet: 0.339 ± 0.013
0.379HisAsn: 0.379 ± 0.013
1.831HisPro: 1.831 ± 0.034
0.599HisGln: 0.599 ± 0.016
2.058HisArg: 2.058 ± 0.039
0.956HisSer: 0.956 ± 0.021
1.289HisThr: 1.289 ± 0.025
1.663HisVal: 1.663 ± 0.027
0.377HisTrp: 0.377 ± 0.014
0.467HisTyr: 0.467 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
4.891IleAla: 4.891 ± 0.05
0.313IleCys: 0.313 ± 0.014
2.141IleAsp: 2.141 ± 0.032
1.988IleGlu: 1.988 ± 0.032
0.666IlePhe: 0.666 ± 0.019
3.684IleGly: 3.684 ± 0.04
0.611IleHis: 0.611 ± 0.017
0.818IleIle: 0.818 ± 0.022
0.758IleLys: 0.758 ± 0.023
2.374IleLeu: 2.374 ± 0.037
0.455IleMet: 0.455 ± 0.015
0.706IleAsn: 0.706 ± 0.022
1.781IlePro: 1.781 ± 0.031
0.707IleGln: 0.707 ± 0.02
2.281IleArg: 2.281 ± 0.038
1.679IleSer: 1.679 ± 0.031
2.188IleThr: 2.188 ± 0.033
2.598IleVal: 2.598 ± 0.031
0.36IleTrp: 0.36 ± 0.013
0.49IleTyr: 0.49 ± 0.016
0.0IleXaa: 0.0 ± 0.0
Lys
3.15LysAla: 3.15 ± 0.055
0.11LysCys: 0.11 ± 0.007
1.365LysAsp: 1.365 ± 0.032
1.228LysGlu: 1.228 ± 0.029
0.482LysPhe: 0.482 ± 0.016
1.874LysGly: 1.874 ± 0.039
0.426LysHis: 0.426 ± 0.015
0.935LysIle: 0.935 ± 0.024
0.831LysLys: 0.831 ± 0.026
2.097LysLeu: 2.097 ± 0.035
0.416LysMet: 0.416 ± 0.016
0.544LysAsn: 0.544 ± 0.02
1.461LysPro: 1.461 ± 0.03
0.713LysGln: 0.713 ± 0.019
1.419LysArg: 1.419 ± 0.03
1.181LysSer: 1.181 ± 0.027
1.368LysThr: 1.368 ± 0.028
1.958LysVal: 1.958 ± 0.034
0.263LysTrp: 0.263 ± 0.011
0.475LysTyr: 0.475 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
15.775LeuAla: 15.775 ± 0.123
0.883LeuCys: 0.883 ± 0.02
6.483LeuAsp: 6.483 ± 0.058
4.78LeuGlu: 4.78 ± 0.06
2.634LeuPhe: 2.634 ± 0.041
9.585LeuGly: 9.585 ± 0.086
2.304LeuHis: 2.304 ± 0.037
3.221LeuIle: 3.221 ± 0.047
2.048LeuLys: 2.048 ± 0.037
11.507LeuLeu: 11.507 ± 0.108
1.657LeuMet: 1.657 ± 0.028
1.648LeuAsn: 1.648 ± 0.03
6.56LeuPro: 6.56 ± 0.069
2.141LeuGln: 2.141 ± 0.034
8.554LeuArg: 8.554 ± 0.079
5.12LeuSer: 5.12 ± 0.059
6.666LeuThr: 6.666 ± 0.06
8.815LeuVal: 8.815 ± 0.074
1.31LeuTrp: 1.31 ± 0.029
1.91LeuTyr: 1.91 ± 0.033
0.0LeuXaa: 0.0 ± 0.0
Met
2.38MetAla: 2.38 ± 0.038
0.135MetCys: 0.135 ± 0.008
0.951MetAsp: 0.951 ± 0.022
0.799MetGlu: 0.799 ± 0.021
0.436MetPhe: 0.436 ± 0.015
1.368MetGly: 1.368 ± 0.029
0.34MetHis: 0.34 ± 0.013
0.661MetIle: 0.661 ± 0.017
0.436MetLys: 0.436 ± 0.017
1.763MetLeu: 1.763 ± 0.028
0.302MetMet: 0.302 ± 0.013
0.45MetAsn: 0.45 ± 0.014
1.143MetPro: 1.143 ± 0.026
0.441MetGln: 0.441 ± 0.014
1.426MetArg: 1.426 ± 0.026
1.315MetSer: 1.315 ± 0.024
1.486MetThr: 1.486 ± 0.025
1.241MetVal: 1.241 ± 0.025
0.225MetTrp: 0.225 ± 0.01
0.326MetTyr: 0.326 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.237AsnAla: 2.237 ± 0.036
0.164AsnCys: 0.164 ± 0.008
0.902AsnAsp: 0.902 ± 0.024
0.844AsnGlu: 0.844 ± 0.02
0.5AsnPhe: 0.5 ± 0.015
1.847AsnGly: 1.847 ± 0.041
0.392AsnHis: 0.392 ± 0.014
0.639AsnIle: 0.639 ± 0.018
0.422AsnLys: 0.422 ± 0.016
1.686AsnLeu: 1.686 ± 0.029
0.296AsnMet: 0.296 ± 0.013
0.429AsnAsn: 0.429 ± 0.018
1.353AsnPro: 1.353 ± 0.024
0.497AsnGln: 0.497 ± 0.019
1.252AsnArg: 1.252 ± 0.023
0.891AsnSer: 0.891 ± 0.025
1.133AsnThr: 1.133 ± 0.029
1.331AsnVal: 1.331 ± 0.029
0.279AsnTrp: 0.279 ± 0.013
0.38AsnTyr: 0.38 ± 0.016
0.0AsnXaa: 0.0 ± 0.0
Pro
9.94ProAla: 9.94 ± 0.086
0.344ProCys: 0.344 ± 0.013
4.188ProAsp: 4.188 ± 0.053
4.358ProGlu: 4.358 ± 0.053
1.538ProPhe: 1.538 ± 0.03
7.136ProGly: 7.136 ± 0.074
1.339ProHis: 1.339 ± 0.031
1.24ProIle: 1.24 ± 0.026
1.237ProLys: 1.237 ± 0.025
5.461ProLeu: 5.461 ± 0.06
1.058ProMet: 1.058 ± 0.022
0.884ProAsn: 0.884 ± 0.024
3.551ProPro: 3.551 ± 0.072
1.979ProGln: 1.979 ± 0.038
3.881ProArg: 3.881 ± 0.052
3.013ProSer: 3.013 ± 0.043
3.02ProThr: 3.02 ± 0.045
5.681ProVal: 5.681 ± 0.059
0.94ProTrp: 0.94 ± 0.026
1.488ProTyr: 1.488 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
3.923GlnAla: 3.923 ± 0.056
0.179GlnCys: 0.179 ± 0.01
1.435GlnAsp: 1.435 ± 0.027
1.473GlnGlu: 1.473 ± 0.032
0.67GlnPhe: 0.67 ± 0.018
2.389GlnGly: 2.389 ± 0.039
0.6GlnHis: 0.6 ± 0.019
1.077GlnIle: 1.077 ± 0.023
0.614GlnLys: 0.614 ± 0.02
2.95GlnLeu: 2.95 ± 0.044
0.497GlnMet: 0.497 ± 0.017
0.511GlnAsn: 0.511 ± 0.02
1.679GlnPro: 1.679 ± 0.034
1.217GlnGln: 1.217 ± 0.039
2.208GlnArg: 2.208 ± 0.032
1.286GlnSer: 1.286 ± 0.024
1.367GlnThr: 1.367 ± 0.026
2.363GlnVal: 2.363 ± 0.035
0.472GlnTrp: 0.472 ± 0.016
0.604GlnTyr: 0.604 ± 0.014
0.0GlnXaa: 0.0 ± 0.0
Arg
10.021ArgAla: 10.021 ± 0.101
0.555ArgCys: 0.555 ± 0.018
3.957ArgAsp: 3.957 ± 0.045
4.821ArgGlu: 4.821 ± 0.056
2.337ArgPhe: 2.337 ± 0.029
5.883ArgGly: 5.883 ± 0.055
2.011ArgHis: 2.011 ± 0.034
3.368ArgIle: 3.368 ± 0.042
1.671ArgLys: 1.671 ± 0.031
8.601ArgLeu: 8.601 ± 0.08
1.726ArgMet: 1.726 ± 0.028
1.266ArgAsn: 1.266 ± 0.023
4.974ArgPro: 4.974 ± 0.055
2.161ArgGln: 2.161 ± 0.033
7.373ArgArg: 7.373 ± 0.077
3.977ArgSer: 3.977 ± 0.041
5.408ArgThr: 5.408 ± 0.05
5.588ArgVal: 5.588 ± 0.055
1.346ArgTrp: 1.346 ± 0.029
1.723ArgTyr: 1.723 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
7.033SerAla: 7.033 ± 0.062
0.404SerCys: 0.404 ± 0.018
2.514SerAsp: 2.514 ± 0.035
2.293SerGlu: 2.293 ± 0.035
1.557SerPhe: 1.557 ± 0.032
5.86SerGly: 5.86 ± 0.066
0.962SerHis: 0.962 ± 0.025
1.389SerIle: 1.389 ± 0.026
1.047SerLys: 1.047 ± 0.026
4.733SerLeu: 4.733 ± 0.06
1.063SerMet: 1.063 ± 0.023
0.837SerAsn: 0.837 ± 0.02
3.188SerPro: 3.188 ± 0.038
1.22SerGln: 1.22 ± 0.027
3.52SerArg: 3.52 ± 0.045
2.654SerSer: 2.654 ± 0.044
2.809SerThr: 2.809 ± 0.042
4.185SerVal: 4.185 ± 0.048
0.883SerTrp: 0.883 ± 0.023
1.236SerTyr: 1.236 ± 0.025
0.0SerXaa: 0.0 ± 0.0
Thr
9.004ThrAla: 9.004 ± 0.068
0.419ThrCys: 0.419 ± 0.015
3.298ThrAsp: 3.298 ± 0.044
3.049ThrGlu: 3.049 ± 0.044
1.512ThrPhe: 1.512 ± 0.029
6.766ThrGly: 6.766 ± 0.063
1.133ThrHis: 1.133 ± 0.024
1.64ThrIle: 1.64 ± 0.03
1.157ThrLys: 1.157 ± 0.026
5.575ThrLeu: 5.575 ± 0.056
0.923ThrMet: 0.923 ± 0.022
0.938ThrAsn: 0.938 ± 0.024
4.12ThrPro: 4.12 ± 0.049
1.286ThrGln: 1.286 ± 0.03
3.753ThrArg: 3.753 ± 0.047
2.906ThrSer: 2.906 ± 0.044
3.635ThrThr: 3.635 ± 0.053
5.765ThrVal: 5.765 ± 0.049
0.879ThrTrp: 0.879 ± 0.022
1.345ThrTyr: 1.345 ± 0.031
0.0ThrXaa: 0.0 ± 0.0
Val
10.568ValAla: 10.568 ± 0.087
0.76ValCys: 0.76 ± 0.019
4.727ValAsp: 4.727 ± 0.049
4.682ValGlu: 4.682 ± 0.048
2.445ValPhe: 2.445 ± 0.041
6.192ValGly: 6.192 ± 0.057
1.942ValHis: 1.942 ± 0.028
2.878ValIle: 2.878 ± 0.038
1.848ValLys: 1.848 ± 0.034
9.584ValLeu: 9.584 ± 0.079
1.46ValMet: 1.46 ± 0.028
1.675ValAsn: 1.675 ± 0.031
5.331ValPro: 5.331 ± 0.052
2.045ValGln: 2.045 ± 0.033
7.114ValArg: 7.114 ± 0.073
4.212ValSer: 4.212 ± 0.046
5.31ValThr: 5.31 ± 0.052
7.678ValVal: 7.678 ± 0.077
1.143ValTrp: 1.143 ± 0.026
1.58ValTyr: 1.58 ± 0.026
0.0ValXaa: 0.0 ± 0.001
Trp
1.738TrpAla: 1.738 ± 0.029
0.156TrpCys: 0.156 ± 0.009
0.805TrpAsp: 0.805 ± 0.022
0.738TrpGlu: 0.738 ± 0.02
0.508TrpPhe: 0.508 ± 0.015
1.052TrpGly: 1.052 ± 0.019
0.357TrpHis: 0.357 ± 0.013
0.568TrpIle: 0.568 ± 0.017
0.385TrpLys: 0.385 ± 0.015
1.776TrpLeu: 1.776 ± 0.033
0.3TrpMet: 0.3 ± 0.012
0.424TrpAsn: 0.424 ± 0.016
0.798TrpPro: 0.798 ± 0.022
0.623TrpGln: 0.623 ± 0.017
1.281TrpArg: 1.281 ± 0.025
0.939TrpSer: 0.939 ± 0.023
1.059TrpThr: 1.059 ± 0.026
0.982TrpVal: 0.982 ± 0.02
0.345TrpTrp: 0.345 ± 0.013
0.36TrpTyr: 0.36 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.921TyrAla: 2.921 ± 0.041
0.185TyrCys: 0.185 ± 0.01
1.415TyrAsp: 1.415 ± 0.029
1.409TyrGlu: 1.409 ± 0.024
0.656TyrPhe: 0.656 ± 0.018
2.374TyrGly: 2.374 ± 0.039
0.391TyrHis: 0.391 ± 0.015
0.444TyrIle: 0.444 ± 0.015
0.417TyrLys: 0.417 ± 0.016
2.086TyrLeu: 2.086 ± 0.037
0.25TyrMet: 0.25 ± 0.011
0.383TyrAsn: 0.383 ± 0.015
1.09TyrPro: 1.09 ± 0.021
0.596TyrGln: 0.596 ± 0.019
1.793TyrArg: 1.793 ± 0.029
0.96TyrSer: 0.96 ± 0.025
1.148TyrThr: 1.148 ± 0.024
1.673TyrVal: 1.673 ± 0.03
0.364TyrTrp: 0.364 ± 0.013
0.441TyrTyr: 0.441 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.001XaaLeu: 0.001 ± 0.001
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.007XaaXaa: 0.007 ± 0.003
Statistics based on 6493 proteins (2083404 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski