Amino acid dipepetide frequency for Sphingomonas sp. Leaf34

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.049AlaAla: 20.049 ± 0.197
1.016AlaCys: 1.016 ± 0.034
8.11AlaAsp: 8.11 ± 0.108
6.717AlaGlu: 6.717 ± 0.085
4.596AlaPhe: 4.596 ± 0.074
11.845AlaGly: 11.845 ± 0.122
2.341AlaHis: 2.341 ± 0.051
7.504AlaIle: 7.504 ± 0.097
4.469AlaLys: 4.469 ± 0.078
13.898AlaLeu: 13.898 ± 0.151
3.923AlaMet: 3.923 ± 0.053
3.44AlaAsn: 3.44 ± 0.064
6.667AlaPro: 6.667 ± 0.094
4.476AlaGln: 4.476 ± 0.072
9.807AlaArg: 9.807 ± 0.113
6.722AlaSer: 6.722 ± 0.078
8.243AlaThr: 8.243 ± 0.088
9.505AlaVal: 9.505 ± 0.095
1.756AlaTrp: 1.756 ± 0.047
2.799AlaTyr: 2.799 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.941CysAla: 0.941 ± 0.032
0.076CysCys: 0.076 ± 0.009
0.481CysAsp: 0.481 ± 0.02
0.328CysGlu: 0.328 ± 0.017
0.247CysPhe: 0.247 ± 0.017
0.746CysGly: 0.746 ± 0.025
0.151CysHis: 0.151 ± 0.013
0.331CysIle: 0.331 ± 0.015
0.158CysLys: 0.158 ± 0.012
0.602CysLeu: 0.602 ± 0.027
0.132CysMet: 0.132 ± 0.011
0.185CysAsn: 0.185 ± 0.011
0.381CysPro: 0.381 ± 0.019
0.148CysGln: 0.148 ± 0.012
0.463CysArg: 0.463 ± 0.023
0.375CysSer: 0.375 ± 0.019
0.393CysThr: 0.393 ± 0.02
0.546CysVal: 0.546 ± 0.024
0.102CysTrp: 0.102 ± 0.009
0.152CysTyr: 0.152 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
8.591AspAla: 8.591 ± 0.106
0.423AspCys: 0.423 ± 0.021
3.594AspAsp: 3.594 ± 0.058
3.054AspGlu: 3.054 ± 0.058
2.073AspPhe: 2.073 ± 0.043
5.865AspGly: 5.865 ± 0.085
1.294AspHis: 1.294 ± 0.041
2.92AspIle: 2.92 ± 0.052
1.574AspLys: 1.574 ± 0.041
5.768AspLeu: 5.768 ± 0.071
1.352AspMet: 1.352 ± 0.034
1.253AspAsn: 1.253 ± 0.037
3.888AspPro: 3.888 ± 0.062
1.874AspGln: 1.874 ± 0.048
5.229AspArg: 5.229 ± 0.075
2.389AspSer: 2.389 ± 0.045
3.228AspThr: 3.228 ± 0.055
4.413AspVal: 4.413 ± 0.06
1.113AspTrp: 1.113 ± 0.035
1.564AspTyr: 1.564 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
6.78GluAla: 6.78 ± 0.084
0.252GluCys: 0.252 ± 0.016
2.473GluAsp: 2.473 ± 0.054
2.055GluGlu: 2.055 ± 0.048
1.294GluPhe: 1.294 ± 0.039
3.9GluGly: 3.9 ± 0.069
1.026GluHis: 1.026 ± 0.032
2.527GluIle: 2.527 ± 0.055
1.534GluLys: 1.534 ± 0.038
4.133GluLeu: 4.133 ± 0.076
1.176GluMet: 1.176 ± 0.036
1.122GluAsn: 1.122 ± 0.034
2.293GluPro: 2.293 ± 0.048
1.756GluGln: 1.756 ± 0.047
4.452GluArg: 4.452 ± 0.068
2.09GluSer: 2.09 ± 0.043
3.192GluThr: 3.192 ± 0.061
3.092GluVal: 3.092 ± 0.052
0.656GluTrp: 0.656 ± 0.024
0.889GluTyr: 0.889 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
5.148PheAla: 5.148 ± 0.071
0.276PheCys: 0.276 ± 0.016
2.721PheAsp: 2.721 ± 0.052
1.789PheGlu: 1.789 ± 0.043
1.21PhePhe: 1.21 ± 0.038
3.765PheGly: 3.765 ± 0.068
0.668PheHis: 0.668 ± 0.025
1.278PheIle: 1.278 ± 0.041
0.902PheLys: 0.902 ± 0.033
3.004PheLeu: 3.004 ± 0.057
0.695PheMet: 0.695 ± 0.027
0.97PheAsn: 0.97 ± 0.033
1.395PhePro: 1.395 ± 0.037
0.849PheGln: 0.849 ± 0.028
2.122PheArg: 2.122 ± 0.041
1.851PheSer: 1.851 ± 0.037
2.042PheThr: 2.042 ± 0.044
2.808PheVal: 2.808 ± 0.054
0.473PheTrp: 0.473 ± 0.023
0.892PheTyr: 0.892 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
10.523GlyAla: 10.523 ± 0.11
0.757GlyCys: 0.757 ± 0.03
5.166GlyAsp: 5.166 ± 0.069
4.294GlyGlu: 4.294 ± 0.069
3.696GlyPhe: 3.696 ± 0.06
8.595GlyGly: 8.595 ± 0.137
1.832GlyHis: 1.832 ± 0.041
4.83GlyIle: 4.83 ± 0.071
3.026GlyLys: 3.026 ± 0.061
8.602GlyLeu: 8.602 ± 0.101
2.238GlyMet: 2.238 ± 0.046
2.377GlyAsn: 2.377 ± 0.048
3.579GlyPro: 3.579 ± 0.058
2.915GlyGln: 2.915 ± 0.054
6.387GlyArg: 6.387 ± 0.085
4.823GlySer: 4.823 ± 0.073
5.492GlyThr: 5.492 ± 0.083
6.758GlyVal: 6.758 ± 0.091
1.664GlyTrp: 1.664 ± 0.047
2.488GlyTyr: 2.488 ± 0.056
0.0GlyXaa: 0.0 ± 0.0
His
2.613HisAla: 2.613 ± 0.061
0.173HisCys: 0.173 ± 0.012
1.279HisAsp: 1.279 ± 0.038
0.9HisGlu: 0.9 ± 0.028
0.697HisPhe: 0.697 ± 0.024
1.9HisGly: 1.9 ± 0.046
0.563HisHis: 0.563 ± 0.025
0.88HisIle: 0.88 ± 0.029
0.39HisLys: 0.39 ± 0.019
1.808HisLeu: 1.808 ± 0.044
0.403HisMet: 0.403 ± 0.02
0.406HisAsn: 0.406 ± 0.02
1.23HisPro: 1.23 ± 0.033
0.499HisGln: 0.499 ± 0.02
1.419HisArg: 1.419 ± 0.032
0.76HisSer: 0.76 ± 0.027
0.765HisThr: 0.765 ± 0.027
1.43HisVal: 1.43 ± 0.038
0.334HisTrp: 0.334 ± 0.018
0.48HisTyr: 0.48 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
8.715IleAla: 8.715 ± 0.096
0.367IleCys: 0.367 ± 0.02
4.127IleAsp: 4.127 ± 0.067
3.187IleGlu: 3.187 ± 0.057
1.38IlePhe: 1.38 ± 0.037
5.589IleGly: 5.589 ± 0.084
0.858IleHis: 0.858 ± 0.027
1.738IleIle: 1.738 ± 0.037
1.189IleLys: 1.189 ± 0.037
4.195IleLeu: 4.195 ± 0.065
0.802IleMet: 0.802 ± 0.029
1.186IleAsn: 1.186 ± 0.033
2.264IlePro: 2.264 ± 0.051
1.082IleGln: 1.082 ± 0.034
3.132IleArg: 3.132 ± 0.052
2.24IleSer: 2.24 ± 0.044
2.719IleThr: 2.719 ± 0.047
4.811IleVal: 4.811 ± 0.079
0.548IleTrp: 0.548 ± 0.023
0.894IleTyr: 0.894 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
3.876LysAla: 3.876 ± 0.074
0.123LysCys: 0.123 ± 0.012
1.527LysAsp: 1.527 ± 0.042
0.91LysGlu: 0.91 ± 0.032
0.871LysPhe: 0.871 ± 0.029
2.442LysGly: 2.442 ± 0.052
0.502LysHis: 0.502 ± 0.023
1.384LysIle: 1.384 ± 0.038
0.936LysLys: 0.936 ± 0.039
3.098LysLeu: 3.098 ± 0.057
0.737LysMet: 0.737 ± 0.026
0.69LysAsn: 0.69 ± 0.027
2.146LysPro: 2.146 ± 0.051
0.881LysGln: 0.881 ± 0.03
2.158LysArg: 2.158 ± 0.046
1.592LysSer: 1.592 ± 0.035
1.918LysThr: 1.918 ± 0.044
2.094LysVal: 2.094 ± 0.053
0.35LysTrp: 0.35 ± 0.016
0.593LysTyr: 0.593 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
14.273LeuAla: 14.273 ± 0.146
0.686LeuCys: 0.686 ± 0.024
6.297LeuAsp: 6.297 ± 0.074
4.103LeuGlu: 4.103 ± 0.064
3.454LeuPhe: 3.454 ± 0.066
8.396LeuGly: 8.396 ± 0.098
1.75LeuHis: 1.75 ± 0.049
4.724LeuIle: 4.724 ± 0.066
2.953LeuLys: 2.953 ± 0.057
8.94LeuLeu: 8.94 ± 0.136
2.011LeuMet: 2.011 ± 0.041
2.293LeuAsn: 2.293 ± 0.049
5.544LeuPro: 5.544 ± 0.082
2.431LeuGln: 2.431 ± 0.05
6.521LeuArg: 6.521 ± 0.086
5.603LeuSer: 5.603 ± 0.079
5.851LeuThr: 5.851 ± 0.07
7.622LeuVal: 7.622 ± 0.094
1.22LeuTrp: 1.22 ± 0.04
2.028LeuTyr: 2.028 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
3.169MetAla: 3.169 ± 0.062
0.128MetCys: 0.128 ± 0.011
1.008MetAsp: 1.008 ± 0.028
0.821MetGlu: 0.821 ± 0.034
0.702MetPhe: 0.702 ± 0.023
1.732MetGly: 1.732 ± 0.046
0.402MetHis: 0.402 ± 0.016
1.499MetIle: 1.499 ± 0.037
0.872MetLys: 0.872 ± 0.029
2.721MetLeu: 2.721 ± 0.053
0.657MetMet: 0.657 ± 0.027
0.648MetAsn: 0.648 ± 0.025
1.502MetPro: 1.502 ± 0.033
0.63MetGln: 0.63 ± 0.026
1.709MetArg: 1.709 ± 0.037
1.303MetSer: 1.303 ± 0.038
2.03MetThr: 2.03 ± 0.046
1.634MetVal: 1.634 ± 0.041
0.204MetTrp: 0.204 ± 0.014
0.29MetTyr: 0.29 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
3.458AsnAla: 3.458 ± 0.068
0.205AsnCys: 0.205 ± 0.013
1.489AsnAsp: 1.489 ± 0.035
0.968AsnGlu: 0.968 ± 0.026
0.904AsnPhe: 0.904 ± 0.031
2.499AsnGly: 2.499 ± 0.059
0.452AsnHis: 0.452 ± 0.018
1.223AsnIle: 1.223 ± 0.039
0.605AsnLys: 0.605 ± 0.022
2.417AsnLeu: 2.417 ± 0.056
0.477AsnMet: 0.477 ± 0.024
0.649AsnAsn: 0.649 ± 0.026
1.656AsnPro: 1.656 ± 0.044
0.726AsnGln: 0.726 ± 0.028
1.781AsnArg: 1.781 ± 0.04
1.145AsnSer: 1.145 ± 0.036
1.326AsnThr: 1.326 ± 0.039
1.928AsnVal: 1.928 ± 0.047
0.411AsnTrp: 0.411 ± 0.02
0.705AsnTyr: 0.705 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
7.288ProAla: 7.288 ± 0.094
0.28ProCys: 0.28 ± 0.016
3.73ProAsp: 3.73 ± 0.069
2.97ProGlu: 2.97 ± 0.053
1.893ProPhe: 1.893 ± 0.044
4.669ProGly: 4.669 ± 0.061
0.969ProHis: 0.969 ± 0.031
2.715ProIle: 2.715 ± 0.046
1.57ProLys: 1.57 ± 0.04
4.83ProLeu: 4.83 ± 0.072
1.241ProMet: 1.241 ± 0.03
1.358ProAsn: 1.358 ± 0.038
2.62ProPro: 2.62 ± 0.058
1.585ProGln: 1.585 ± 0.038
3.091ProArg: 3.091 ± 0.067
2.856ProSer: 2.856 ± 0.056
3.042ProThr: 3.042 ± 0.053
4.561ProVal: 4.561 ± 0.064
0.695ProTrp: 0.695 ± 0.027
1.091ProTyr: 1.091 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
4.038GlnAla: 4.038 ± 0.059
0.194GlnCys: 0.194 ± 0.014
1.379GlnAsp: 1.379 ± 0.035
0.999GlnGlu: 0.999 ± 0.031
1.062GlnPhe: 1.062 ± 0.034
2.453GlnGly: 2.453 ± 0.046
0.53GlnHis: 0.53 ± 0.025
1.675GlnIle: 1.675 ± 0.041
0.785GlnLys: 0.785 ± 0.026
2.769GlnLeu: 2.769 ± 0.053
0.797GlnMet: 0.797 ± 0.025
0.725GlnAsn: 0.725 ± 0.03
1.738GlnPro: 1.738 ± 0.043
1.033GlnGln: 1.033 ± 0.036
2.413GlnArg: 2.413 ± 0.052
1.766GlnSer: 1.766 ± 0.044
1.923GlnThr: 1.923 ± 0.046
2.295GlnVal: 2.295 ± 0.049
0.387GlnTrp: 0.387 ± 0.018
0.683GlnTyr: 0.683 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
8.976ArgAla: 8.976 ± 0.107
0.459ArgCys: 0.459 ± 0.022
4.565ArgAsp: 4.565 ± 0.062
3.398ArgGlu: 3.398 ± 0.056
3.036ArgPhe: 3.036 ± 0.059
5.171ArgGly: 5.171 ± 0.077
1.566ArgHis: 1.566 ± 0.038
4.182ArgIle: 4.182 ± 0.068
1.854ArgLys: 1.854 ± 0.042
7.684ArgLeu: 7.684 ± 0.098
2.026ArgMet: 2.026 ± 0.048
1.797ArgAsn: 1.797 ± 0.044
3.539ArgPro: 3.539 ± 0.064
2.247ArgGln: 2.247 ± 0.046
5.496ArgArg: 5.496 ± 0.092
3.621ArgSer: 3.621 ± 0.066
4.106ArgThr: 4.106 ± 0.063
4.934ArgVal: 4.934 ± 0.069
1.225ArgTrp: 1.225 ± 0.037
2.029ArgTyr: 2.029 ± 0.046
0.0ArgXaa: 0.0 ± 0.0
Ser
6.374SerAla: 6.374 ± 0.077
0.335SerCys: 0.335 ± 0.018
3.035SerAsp: 3.035 ± 0.055
2.178SerGlu: 2.178 ± 0.04
2.063SerPhe: 2.063 ± 0.045
5.365SerGly: 5.365 ± 0.072
0.871SerHis: 0.871 ± 0.031
2.721SerIle: 2.721 ± 0.048
1.409SerLys: 1.409 ± 0.034
4.936SerLeu: 4.936 ± 0.067
1.174SerMet: 1.174 ± 0.031
1.398SerAsn: 1.398 ± 0.04
2.829SerPro: 2.829 ± 0.057
1.418SerGln: 1.418 ± 0.037
3.236SerArg: 3.236 ± 0.049
2.645SerSer: 2.645 ± 0.067
2.982SerThr: 2.982 ± 0.059
4.003SerVal: 4.003 ± 0.064
0.684SerTrp: 0.684 ± 0.027
1.361SerTyr: 1.361 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
7.61ThrAla: 7.61 ± 0.094
0.367ThrCys: 0.367 ± 0.02
3.384ThrAsp: 3.384 ± 0.059
2.351ThrGlu: 2.351 ± 0.047
1.842ThrPhe: 1.842 ± 0.037
5.919ThrGly: 5.919 ± 0.082
1.006ThrHis: 1.006 ± 0.032
3.579ThrIle: 3.579 ± 0.057
1.534ThrLys: 1.534 ± 0.043
6.717ThrLeu: 6.717 ± 0.09
1.399ThrMet: 1.399 ± 0.035
1.504ThrAsn: 1.504 ± 0.054
3.966ThrPro: 3.966 ± 0.063
1.759ThrGln: 1.759 ± 0.043
3.931ThrArg: 3.931 ± 0.063
2.992ThrSer: 2.992 ± 0.052
3.486ThrThr: 3.486 ± 0.066
4.664ThrVal: 4.664 ± 0.069
0.677ThrTrp: 0.677 ± 0.026
1.304ThrTyr: 1.304 ± 0.037
0.001ThrXaa: 0.001 ± 0.001
Val
10.944ValAla: 10.944 ± 0.112
0.503ValCys: 0.503 ± 0.021
4.729ValAsp: 4.729 ± 0.064
4.143ValGlu: 4.143 ± 0.066
2.441ValPhe: 2.441 ± 0.048
6.047ValGly: 6.047 ± 0.066
1.332ValHis: 1.332 ± 0.037
3.782ValIle: 3.782 ± 0.069
2.058ValLys: 2.058 ± 0.05
6.832ValLeu: 6.832 ± 0.09
1.72ValMet: 1.72 ± 0.04
1.935ValAsn: 1.935 ± 0.05
4.058ValPro: 4.058 ± 0.07
2.081ValGln: 2.081 ± 0.046
5.313ValArg: 5.313 ± 0.077
4.193ValSer: 4.193 ± 0.058
5.157ValThr: 5.157 ± 0.072
6.007ValVal: 6.007 ± 0.072
0.89ValTrp: 0.89 ± 0.027
1.473ValTyr: 1.473 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
1.43TrpAla: 1.43 ± 0.038
0.106TrpCys: 0.106 ± 0.011
0.71TrpAsp: 0.71 ± 0.023
0.479TrpGlu: 0.479 ± 0.023
0.558TrpPhe: 0.558 ± 0.023
0.927TrpGly: 0.927 ± 0.032
0.325TrpHis: 0.325 ± 0.018
0.686TrpIle: 0.686 ± 0.028
0.422TrpLys: 0.422 ± 0.018
1.705TrpLeu: 1.705 ± 0.052
0.315TrpMet: 0.315 ± 0.017
0.467TrpAsn: 0.467 ± 0.022
0.705TrpPro: 0.705 ± 0.028
0.551TrpGln: 0.551 ± 0.023
1.321TrpArg: 1.321 ± 0.035
0.911TrpSer: 0.911 ± 0.03
0.955TrpThr: 0.955 ± 0.034
0.851TrpVal: 0.851 ± 0.033
0.276TrpTrp: 0.276 ± 0.018
0.317TrpTyr: 0.317 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.088TyrAla: 3.088 ± 0.06
0.21TyrCys: 0.21 ± 0.012
1.655TyrAsp: 1.655 ± 0.038
1.047TyrGlu: 1.047 ± 0.036
0.838TyrPhe: 0.838 ± 0.031
2.217TyrGly: 2.217 ± 0.051
0.49TyrHis: 0.49 ± 0.02
0.846TyrIle: 0.846 ± 0.027
0.587TyrLys: 0.587 ± 0.023
2.042TyrLeu: 2.042 ± 0.049
0.384TyrMet: 0.384 ± 0.018
0.624TyrAsn: 0.624 ± 0.027
1.055TyrPro: 1.055 ± 0.029
0.707TyrGln: 0.707 ± 0.033
1.926TyrArg: 1.926 ± 0.043
1.138TyrSer: 1.138 ± 0.038
1.139TyrThr: 1.139 ± 0.037
1.686TyrVal: 1.686 ± 0.044
0.351TyrTrp: 0.351 ± 0.018
0.657TyrTyr: 0.657 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.001XaaCys: 0.001 ± 0.001
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3438 proteins (1126609 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski