Tsuneonella dongtanensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Erythrobacteraceae; Tsuneonella

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2897 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B2AA91|A0A1B2AA91_9SPHN Uncharacterized protein OS=Tsuneonella dongtanensis OX=692370 GN=A6F68_00545 PE=4 SV=1
MM1 pKa = 7.61RR2 pKa = 11.84KK3 pKa = 9.04IAVTAALAAALAATPSLAAEE23 pKa = 4.3KK24 pKa = 9.02TADD27 pKa = 3.52MEE29 pKa = 4.39VTLNVLNNCTLQTPAALDD47 pKa = 3.8FGTVTDD53 pKa = 3.87VTTATASGTATVNCTIDD70 pKa = 3.08TAYY73 pKa = 10.72DD74 pKa = 3.42LSISMGEE81 pKa = 3.99NEE83 pKa = 4.19SGGFRR88 pKa = 11.84RR89 pKa = 11.84MANADD94 pKa = 2.87ATAFVPYY101 pKa = 9.87YY102 pKa = 10.22IEE104 pKa = 5.07IDD106 pKa = 3.72GSSGALSGTGTGADD120 pKa = 3.65VDD122 pKa = 3.89HH123 pKa = 6.93SLAGVLSPSGPVAADD138 pKa = 3.65SYY140 pKa = 11.53ADD142 pKa = 3.65LVVVSLQYY150 pKa = 11.37

Molecular weight: 15.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B2A908|A0A1B2A908_9SPHN UDP-glucose 4-epimerase OS=Tsuneonella dongtanensis OX=692370 GN=galE PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.89GFFARR21 pKa = 11.84KK22 pKa = 7.42ATPGGRR28 pKa = 11.84KK29 pKa = 8.0VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.81KK41 pKa = 10.46LCAA44 pKa = 3.96

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2897

0

2897

930269

29

2917

321.1

34.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.269 ± 0.073

0.803 ± 0.013

6.104 ± 0.038

5.905 ± 0.045

3.646 ± 0.03

9.102 ± 0.049

1.91 ± 0.022

4.752 ± 0.03

3.045 ± 0.036

9.678 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.409 ± 0.02

2.46 ± 0.037

5.414 ± 0.035

2.857 ± 0.019

7.296 ± 0.05

5.21 ± 0.035

5.196 ± 0.046

7.287 ± 0.036

1.496 ± 0.021

2.162 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski