Vannielia litorea

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Vannielia

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4018 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N6E734|A0A1N6E734_9RHOB Uncharacterized protein OS=Vannielia litorea OX=1217970 GN=SAMN05444002_0409 PE=4 SV=1
MM1 pKa = 7.4LLDD4 pKa = 4.08EE5 pKa = 4.68KK6 pKa = 11.35SEE8 pKa = 4.33IIAHH12 pKa = 6.62FIGLFQLGSEE22 pKa = 4.47AARR25 pKa = 11.84MRR27 pKa = 11.84EE28 pKa = 3.92EE29 pKa = 4.03FEE31 pKa = 4.07EE32 pKa = 4.5FQALKK37 pKa = 10.79NADD40 pKa = 3.84PEE42 pKa = 4.55FGSLLNITINLFSDD56 pKa = 4.05YY57 pKa = 10.57EE58 pKa = 4.24LGAFTPDD65 pKa = 2.83IQGPLYY71 pKa = 10.52FPVLPGVPGLPYY83 pKa = 10.77LPFAGPAAEE92 pKa = 4.57HH93 pKa = 6.67LWPVLPGEE101 pKa = 4.08LAEE104 pKa = 5.42LPIYY108 pKa = 9.55WSPPGYY114 pKa = 10.75LLAKK118 pKa = 9.81LQFLIPPPSSVATITVQYY136 pKa = 11.03NFLRR140 pKa = 11.84DD141 pKa = 3.24YY142 pKa = 11.03DD143 pKa = 4.07YY144 pKa = 11.82ASTSDD149 pKa = 3.64AEE151 pKa = 4.3MSFVDD156 pKa = 4.25PVAFEE161 pKa = 4.6VPLSSLQATATALQPFSAPVMPEE184 pKa = 3.57DD185 pKa = 3.48EE186 pKa = 4.22AAIAEE191 pKa = 4.42SGRR194 pKa = 11.84EE195 pKa = 3.72VSEE198 pKa = 5.1DD199 pKa = 2.8IRR201 pKa = 11.84ALEE204 pKa = 4.23SEE206 pKa = 4.65GGPGAADD213 pKa = 3.45GAVTFVAFGEE223 pKa = 4.4ATATPTLNGLAVEE236 pKa = 4.58EE237 pKa = 4.28MPKK240 pKa = 10.69LSDD243 pKa = 3.62YY244 pKa = 11.33SDD246 pKa = 3.43TFAEE250 pKa = 4.5PEE252 pKa = 4.13PEE254 pKa = 4.11PEE256 pKa = 4.15EE257 pKa = 4.13EE258 pKa = 4.33VPEE261 pKa = 5.12SPFEE265 pKa = 4.49DD266 pKa = 3.49NRR268 pKa = 11.84DD269 pKa = 3.44EE270 pKa = 5.42AEE272 pKa = 4.24EE273 pKa = 4.51DD274 pKa = 3.47DD275 pKa = 4.56HH276 pKa = 9.11PEE278 pKa = 4.22GQHH281 pKa = 4.08VTMGEE286 pKa = 3.85NLLINQANVVMNWLDD301 pKa = 3.41APVFVVMGDD310 pKa = 3.5VVVANAISQINVWNDD325 pKa = 2.85FDD327 pKa = 4.32KK328 pKa = 11.53VSGVSSLTEE337 pKa = 4.04AGSTRR342 pKa = 11.84SVNASSIEE350 pKa = 4.1HH351 pKa = 5.6VANPVSVGEE360 pKa = 4.13GAYY363 pKa = 10.49GPGQAIVTRR372 pKa = 11.84IEE374 pKa = 4.19GNVINYY380 pKa = 7.8NHH382 pKa = 6.47IQQFNFAAEE391 pKa = 4.19GDD393 pKa = 4.18VLSLLFNASEE403 pKa = 4.42TFIQTGGNTLFNIADD418 pKa = 3.52ILEE421 pKa = 4.51LGFGYY426 pKa = 10.67DD427 pKa = 4.47LIVIGGNMIDD437 pKa = 3.74VTLLTQMNVLLDD449 pKa = 3.96ADD451 pKa = 3.74SAEE454 pKa = 4.19IEE456 pKa = 4.48GEE458 pKa = 4.02FGGTMDD464 pKa = 3.85TSGNLLLNYY473 pKa = 10.11AAITEE478 pKa = 4.37VGVDD482 pKa = 3.23TTVAITEE489 pKa = 4.61AYY491 pKa = 9.97SKK493 pKa = 10.77AANKK497 pKa = 9.73VIEE500 pKa = 4.56GGEE503 pKa = 3.97PGGDD507 pKa = 2.93ILGDD511 pKa = 3.51PAYY514 pKa = 10.7LGTPVLRR521 pKa = 11.84VLYY524 pKa = 10.43IEE526 pKa = 5.33GDD528 pKa = 3.81YY529 pKa = 11.35LDD531 pKa = 3.73VQVIEE536 pKa = 4.19QVNVLGDD543 pKa = 3.53VDD545 pKa = 4.27QVALAAEE552 pKa = 4.37TLLSAEE558 pKa = 4.35GADD561 pKa = 3.52VTVLTGGNEE570 pKa = 4.22LVNIASVTDD579 pKa = 3.54HH580 pKa = 6.69GVDD583 pKa = 3.32STVYY587 pKa = 9.4TGGEE591 pKa = 4.13AYY593 pKa = 10.45SDD595 pKa = 3.34AMLYY599 pKa = 9.39QAEE602 pKa = 5.09FISNDD607 pKa = 3.47DD608 pKa = 3.75PFALASSDD616 pKa = 3.57NLASEE621 pKa = 4.74AVLFLADD628 pKa = 5.55DD629 pKa = 4.12MLSDD633 pKa = 3.74TSDD636 pKa = 3.5GPGAGLGQLVPEE648 pKa = 4.52EE649 pKa = 4.46APVDD653 pKa = 3.87VMQTLVAA660 pKa = 4.55

Molecular weight: 70.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N6ICQ0|A0A1N6ICQ0_9RHOB Putative membrane protein insertion efficiency factor OS=Vannielia litorea OX=1217970 GN=SAMN05444002_3711 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4018

0

4018

1242426

29

5927

309.2

33.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.271 ± 0.064

0.895 ± 0.014

5.439 ± 0.035

6.466 ± 0.04

3.642 ± 0.029

9.158 ± 0.043

2.003 ± 0.02

4.715 ± 0.032

2.936 ± 0.03

10.192 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.709 ± 0.021

2.355 ± 0.03

5.347 ± 0.039

2.89 ± 0.019

6.821 ± 0.044

4.884 ± 0.032

5.368 ± 0.044

7.329 ± 0.034

1.452 ± 0.018

2.131 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski