Gordonia phage Confidence

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1A0Q4|A0A2P1A0Q4_9CAUD Uncharacterized protein OS=Gordonia phage Confidence OX=2081621 GN=47 PE=4 SV=1
MM1 pKa = 6.78TTPPPTVILPEE12 pKa = 4.12YY13 pKa = 9.66PGVTFTMLTGSGGVPGLPSNAIRR36 pKa = 11.84IVGTVVNPWYY46 pKa = 10.22EE47 pKa = 3.47PGYY50 pKa = 11.14NYY52 pKa = 10.89GQDD55 pKa = 3.41PNGFTEE61 pKa = 4.97VTDD64 pKa = 3.14PWKK67 pKa = 10.71RR68 pKa = 11.84HH69 pKa = 4.33TEE71 pKa = 4.27LIEE74 pKa = 3.91VTNTGFLAPTAPVPTEE90 pKa = 4.44PPPSSPTTEE99 pKa = 4.49PEE101 pKa = 4.07SEE103 pKa = 4.23PTPEE107 pKa = 3.95EE108 pKa = 3.97PP109 pKa = 4.99

Molecular weight: 11.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1A0S8|A0A2P1A0S8_9CAUD Uncharacterized protein OS=Gordonia phage Confidence OX=2081621 GN=67 PE=4 SV=1
MM1 pKa = 7.27TARR4 pKa = 11.84PCIEE8 pKa = 4.54PGCDD12 pKa = 2.63KK13 pKa = 10.56PARR16 pKa = 11.84TRR18 pKa = 11.84GKK20 pKa = 10.55CPACYY25 pKa = 9.93EE26 pKa = 4.13RR27 pKa = 11.84DD28 pKa = 3.32RR29 pKa = 11.84TRR31 pKa = 11.84QKK33 pKa = 11.28AYY35 pKa = 10.42GRR37 pKa = 11.84WEE39 pKa = 4.18SQHH42 pKa = 7.38VDD44 pKa = 3.06AQPVRR49 pKa = 11.84KK50 pKa = 8.93HH51 pKa = 3.99VHH53 pKa = 5.77ALRR56 pKa = 11.84EE57 pKa = 4.01AGISNKK63 pKa = 8.92RR64 pKa = 11.84LRR66 pKa = 11.84EE67 pKa = 3.98LTGVSTNTIQVLMTGRR83 pKa = 11.84PEE85 pKa = 4.32RR86 pKa = 11.84GHH88 pKa = 6.41GPSKK92 pKa = 10.44KK93 pKa = 9.72VLRR96 pKa = 11.84RR97 pKa = 11.84TADD100 pKa = 4.01RR101 pKa = 11.84ILSVPVPEE109 pKa = 4.35IAFAVASPGRR119 pKa = 11.84AVPALGTTRR128 pKa = 11.84RR129 pKa = 11.84LQALVANGYY138 pKa = 7.26SQRR141 pKa = 11.84DD142 pKa = 3.44LCRR145 pKa = 11.84RR146 pKa = 11.84LGWAWDD152 pKa = 3.77TNATDD157 pKa = 4.32LFLGRR162 pKa = 11.84AQQVTAGRR170 pKa = 11.84ARR172 pKa = 11.84AVAALFVEE180 pKa = 4.75LQMVPGTDD188 pKa = 2.75VRR190 pKa = 11.84ARR192 pKa = 11.84NRR194 pKa = 11.84ARR196 pKa = 11.84AKK198 pKa = 10.41GWLLPLDD205 pKa = 3.93WDD207 pKa = 4.36EE208 pKa = 5.48DD209 pKa = 4.47TIDD212 pKa = 4.74DD213 pKa = 4.68PNHH216 pKa = 6.18TPHH219 pKa = 6.28TPARR223 pKa = 11.84ASASGSRR230 pKa = 11.84MATTLDD236 pKa = 3.25EE237 pKa = 4.92FEE239 pKa = 3.94WLLRR243 pKa = 11.84CGEE246 pKa = 4.24VPDD249 pKa = 4.5IAAKK253 pKa = 9.71RR254 pKa = 11.84CRR256 pKa = 11.84VSLSTIRR263 pKa = 11.84IYY265 pKa = 10.76YY266 pKa = 9.12SRR268 pKa = 11.84EE269 pKa = 3.59GRR271 pKa = 11.84DD272 pKa = 3.26VPTQLAPAPALPIAYY287 pKa = 8.18PRR289 pKa = 11.84ARR291 pKa = 11.84RR292 pKa = 11.84SQVASS297 pKa = 3.46

Molecular weight: 32.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

16122

25

1884

204.1

22.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.518 ± 0.575

0.986 ± 0.154

7.052 ± 0.286

5.831 ± 0.28

2.828 ± 0.154

8.64 ± 0.442

1.966 ± 0.231

4.652 ± 0.213

3.263 ± 0.232

7.729 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.072 ± 0.116

3.002 ± 0.194

5.626 ± 0.326

3.808 ± 0.425

7.487 ± 0.329

5.421 ± 0.277

6.972 ± 0.307

6.792 ± 0.262

2.159 ± 0.168

2.196 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski