Pacific flying fox faeces associated circular DNA virus-14

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140CTW2|A0A140CTW2_9VIRU Replication-associated protein OS=Pacific flying fox faeces associated circular DNA virus-14 OX=1796009 PE=3 SV=1
MM1 pKa = 7.49NSVCTLSHH9 pKa = 7.12RR10 pKa = 11.84STSLLSATTTVTDD23 pKa = 3.38PHH25 pKa = 5.76TTTTEE30 pKa = 3.8TTRR33 pKa = 11.84PDD35 pKa = 2.99IVIRR39 pKa = 11.84PDD41 pKa = 3.38TLISANRR48 pKa = 11.84NQDD51 pKa = 3.48DD52 pKa = 3.98EE53 pKa = 4.87TGLEE57 pKa = 4.63PIPDD61 pKa = 3.28KK62 pKa = 11.2NFRR65 pKa = 11.84LNCKK69 pKa = 9.83AVFLTYY75 pKa = 8.8PQCALEE81 pKa = 4.47PEE83 pKa = 4.85HH84 pKa = 7.2VLQTILACNKK94 pKa = 8.17TKK96 pKa = 10.21HH97 pKa = 6.28CSVIVAQEE105 pKa = 3.7HH106 pKa = 6.38HH107 pKa = 6.91KK108 pKa = 11.07DD109 pKa = 3.8GNKK112 pKa = 9.62HH113 pKa = 4.28LHH115 pKa = 6.27VYY117 pKa = 10.92LEE119 pKa = 4.23FPKK122 pKa = 10.8KK123 pKa = 9.92IDD125 pKa = 3.17IKK127 pKa = 7.71TARR130 pKa = 11.84YY131 pKa = 8.76FNYY134 pKa = 8.73ITGKK138 pKa = 8.93QGNYY142 pKa = 7.83QKK144 pKa = 11.1VKK146 pKa = 10.6VRR148 pKa = 11.84GACIKK153 pKa = 9.32YY154 pKa = 8.2VCKK157 pKa = 10.09EE158 pKa = 3.72NRR160 pKa = 11.84YY161 pKa = 8.82VAHH164 pKa = 6.58QIDD167 pKa = 4.6VPTIIQNYY175 pKa = 7.86EE176 pKa = 3.58KK177 pKa = 10.46QQEE180 pKa = 4.39KK181 pKa = 10.49KK182 pKa = 10.42KK183 pKa = 10.87SRR185 pKa = 11.84NQAKK189 pKa = 9.95NKK191 pKa = 9.68RR192 pKa = 11.84VYY194 pKa = 10.74EE195 pKa = 4.21LIKK198 pKa = 10.39EE199 pKa = 4.29GKK201 pKa = 9.85KK202 pKa = 10.23YY203 pKa = 10.91EE204 pKa = 4.25EE205 pKa = 4.89LLLDD209 pKa = 4.23CTTGPYY215 pKa = 10.3CLLHH219 pKa = 6.12GNKK222 pKa = 8.76IKK224 pKa = 11.18NMITDD229 pKa = 4.16FEE231 pKa = 4.69EE232 pKa = 4.21IAMKK236 pKa = 9.79KK237 pKa = 8.6QRR239 pKa = 11.84IADD242 pKa = 4.11RR243 pKa = 11.84PAYY246 pKa = 10.12LHH248 pKa = 6.96LILPGKK254 pKa = 10.32DD255 pKa = 2.8IDD257 pKa = 4.75LLAKK261 pKa = 10.38KK262 pKa = 9.59PFKK265 pKa = 10.91SPQFWIFGPPNVGKK279 pKa = 8.97TSMLQSLFDD288 pKa = 4.12AGLKK292 pKa = 10.45GFEE295 pKa = 4.06IPINNDD301 pKa = 2.74FARR304 pKa = 11.84WDD306 pKa = 3.63DD307 pKa = 3.66EE308 pKa = 4.92LFDD311 pKa = 3.92FAYY314 pKa = 9.71IDD316 pKa = 3.69EE317 pKa = 5.06FKK319 pKa = 11.18GQLTIQFLNSFLQGSRR335 pKa = 11.84MALPGKK341 pKa = 9.61YY342 pKa = 10.31VIGGKK347 pKa = 9.3IKK349 pKa = 10.74NKK351 pKa = 10.21NIPTFILSNYY361 pKa = 8.03TPEE364 pKa = 3.85SCYY367 pKa = 10.65KK368 pKa = 10.23NKK370 pKa = 10.25SVYY373 pKa = 10.39DD374 pKa = 4.03LQPLLARR381 pKa = 11.84LDD383 pKa = 3.63IIEE386 pKa = 4.09IQKK389 pKa = 10.85FGDD392 pKa = 3.29IQVICTPPIEE402 pKa = 4.1EE403 pKa = 3.96VMMSEE408 pKa = 4.69FEE410 pKa = 4.64PIGITEE416 pKa = 5.12LYY418 pKa = 10.42SDD420 pKa = 4.04EE421 pKa = 4.82NGNIHH426 pKa = 7.04

Molecular weight: 48.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140CTW2|A0A140CTW2_9VIRU Replication-associated protein OS=Pacific flying fox faeces associated circular DNA virus-14 OX=1796009 PE=3 SV=1
MM1 pKa = 7.55NSNFNYY7 pKa = 10.12FLEE10 pKa = 4.33NYY12 pKa = 9.16KK13 pKa = 10.92LSGKK17 pKa = 9.73LLYY20 pKa = 10.25INPQLFQVFMMSGIPTNPNKK40 pKa = 9.82YY41 pKa = 9.69RR42 pKa = 11.84KK43 pKa = 9.17RR44 pKa = 11.84EE45 pKa = 3.75YY46 pKa = 10.01STISSRR52 pKa = 11.84NYY54 pKa = 10.56SNEE57 pKa = 3.62QEE59 pKa = 5.37DD60 pKa = 5.81DD61 pKa = 3.99YY62 pKa = 11.86QCLYY66 pKa = 11.12FNFRR70 pKa = 11.84RR71 pKa = 11.84QYY73 pKa = 9.27SKK75 pKa = 10.85YY76 pKa = 8.87RR77 pKa = 11.84NRR79 pKa = 11.84FPRR82 pKa = 11.84SYY84 pKa = 10.82GAYY87 pKa = 7.79TRR89 pKa = 11.84RR90 pKa = 11.84AVNVRR95 pKa = 11.84GLSYY99 pKa = 8.49PTGVRR104 pKa = 11.84GFWPGRR110 pKa = 11.84RR111 pKa = 11.84STFRR115 pKa = 11.84RR116 pKa = 11.84ITQNVRR122 pKa = 11.84TPSNRR127 pKa = 11.84IVRR130 pKa = 11.84MTYY133 pKa = 9.92SVPEE137 pKa = 3.95KK138 pKa = 10.79KK139 pKa = 10.54FIDD142 pKa = 3.67VTGTYY147 pKa = 10.71AVTAAGDD154 pKa = 3.73LTLLNGLTQGVSVISRR170 pKa = 11.84VGNKK174 pKa = 8.93IMMRR178 pKa = 11.84SLDD181 pKa = 3.82LRR183 pKa = 11.84WRR185 pKa = 11.84LTGAPFSATPTLPFNMVRR203 pKa = 11.84VMLVWDD209 pKa = 4.29AQPNGVIASAGDD221 pKa = 3.46ILQDD225 pKa = 3.55TTPGTGVVSPQQRR238 pKa = 11.84DD239 pKa = 3.44YY240 pKa = 10.68IQRR243 pKa = 11.84FRR245 pKa = 11.84IIMDD249 pKa = 3.74RR250 pKa = 11.84KK251 pKa = 8.65FTLTNLVANNGPTDD265 pKa = 4.05IIIACDD271 pKa = 3.36QMYY274 pKa = 10.76KK275 pKa = 8.89KK276 pKa = 8.7TKK278 pKa = 10.66LMVTYY283 pKa = 10.52SDD285 pKa = 4.46SNTGLIDD292 pKa = 5.36DD293 pKa = 4.78ISTGALYY300 pKa = 10.65LVTVGLHH307 pKa = 5.69AAAANQGVIEE317 pKa = 4.26YY318 pKa = 8.64FSRR321 pKa = 11.84VRR323 pKa = 11.84YY324 pKa = 9.45YY325 pKa = 11.42DD326 pKa = 3.18NN327 pKa = 4.15

Molecular weight: 37.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

753

327

426

376.5

43.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.781 ± 0.264

1.726 ± 0.703

5.312 ± 0.264

4.382 ± 1.607

4.515 ± 0.045

5.71 ± 0.642

1.726 ± 0.896

7.57 ± 1.11

7.039 ± 2.125

8.101 ± 0.48

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.39 ± 0.614

6.507 ± 0.525

5.179 ± 0.374

4.515 ± 0.148

5.976 ± 2.018

5.445 ± 1.002

7.437 ± 0.517

5.71 ± 1.028

0.664 ± 0.16

5.312 ± 0.893
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski