Methanoculleus taiwanensis

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanomicrobiales; Methanomicrobiaceae; Methanoculleus

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2433 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A498H2Q6|A0A498H2Q6_9EURY Acyl_transf_3 domain-containing protein OS=Methanoculleus taiwanensis OX=1550565 GN=ABH15_04610 PE=4 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84KK3 pKa = 8.94ILLISLVILACIVTLLPSAAAASEE27 pKa = 4.33LPAGGQSSTDD37 pKa = 3.19VSVSTNTSVTSTTDD51 pKa = 3.11VSVTTGAGGGAPAPNGTRR69 pKa = 11.84AVDD72 pKa = 3.24VALAIDD78 pKa = 4.45SSASMRR84 pKa = 11.84NTDD87 pKa = 4.15PDD89 pKa = 3.97DD90 pKa = 4.1LRR92 pKa = 11.84IDD94 pKa = 3.7AARR97 pKa = 11.84LFVDD101 pKa = 5.22LAANDD106 pKa = 4.15DD107 pKa = 4.06LLAIVDD113 pKa = 4.52FDD115 pKa = 3.96HH116 pKa = 7.11AVTVLEE122 pKa = 4.16PLSLVRR128 pKa = 11.84NNRR131 pKa = 11.84TALKK135 pKa = 9.51MAIDD139 pKa = 3.98QVDD142 pKa = 3.3SSGEE146 pKa = 4.09TNIGGGLLASFDD158 pKa = 3.82QLAEE162 pKa = 4.07RR163 pKa = 11.84GIPGHH168 pKa = 6.37GKK170 pKa = 8.51ATVLLTDD177 pKa = 4.49GVHH180 pKa = 5.64NTGTDD185 pKa = 3.5PLAVVPLYY193 pKa = 10.87VEE195 pKa = 4.26QGWPVYY201 pKa = 9.47TVALTDD207 pKa = 4.0FADD210 pKa = 3.8EE211 pKa = 3.91ALMRR215 pKa = 11.84EE216 pKa = 4.63IARR219 pKa = 11.84QTGGRR224 pKa = 11.84YY225 pKa = 9.14YY226 pKa = 10.87SAPTSSALLDD236 pKa = 3.16IYY238 pKa = 11.42NRR240 pKa = 11.84IKK242 pKa = 9.75QTIKK246 pKa = 10.79GQDD249 pKa = 3.3QITSEE254 pKa = 4.16TGTISVNQTVQNIVNIDD271 pKa = 3.34TSVQVVEE278 pKa = 5.61FTCLCPQTTINYY290 pKa = 8.77YY291 pKa = 9.68IYY293 pKa = 9.89YY294 pKa = 9.38PNGTRR299 pKa = 11.84VEE301 pKa = 4.59LDD303 pKa = 3.44PEE305 pKa = 4.66SPTGTEE311 pKa = 4.42SPDD314 pKa = 2.81ITYY317 pKa = 10.18ISSDD321 pKa = 3.28TYY323 pKa = 11.09QIYY326 pKa = 9.57QVNNPVVGTWIEE338 pKa = 4.3VINATDD344 pKa = 3.46ANVTVDD350 pKa = 3.4YY351 pKa = 10.51SSTVAVSATIEE362 pKa = 3.69LAAAIDD368 pKa = 3.92AFSYY372 pKa = 11.18APGEE376 pKa = 4.23PVTVTAEE383 pKa = 4.12VRR385 pKa = 11.84NEE387 pKa = 3.64TAGIEE392 pKa = 4.28NATVTAVFTLPGGEE406 pKa = 4.19NEE408 pKa = 4.37TVLLNGTGNGTYY420 pKa = 10.56AGTFSNTSTTGAYY433 pKa = 8.76QVQVRR438 pKa = 11.84STVGDD443 pKa = 3.38IVRR446 pKa = 11.84QEE448 pKa = 4.13VLDD451 pKa = 3.94FAVTEE456 pKa = 4.11EE457 pKa = 4.65APPVTPTPTMAPNATPATPNATPPPTMTLNEE488 pKa = 4.54TPTMTLNEE496 pKa = 4.72TPTMTPTPTPTVPVEE511 pKa = 4.17QPSGAFLQVTNNSADD526 pKa = 3.37QVNPDD531 pKa = 2.88ISGDD535 pKa = 3.78LVVWQDD541 pKa = 3.26NRR543 pKa = 11.84SGNSDD548 pKa = 2.86IFRR551 pKa = 11.84YY552 pKa = 10.26NLTDD556 pKa = 3.61GAVTQITTDD565 pKa = 3.64PADD568 pKa = 3.42QANPAVSGVSRR579 pKa = 11.84LIVWEE584 pKa = 4.22DD585 pKa = 3.24NRR587 pKa = 11.84NGDD590 pKa = 3.68WDD592 pKa = 3.94IFWYY596 pKa = 10.66SPFSGEE602 pKa = 4.12EE603 pKa = 4.17FPVTTAPGDD612 pKa = 3.35QRR614 pKa = 11.84YY615 pKa = 8.67PSIAFQYY622 pKa = 10.42VCWQDD627 pKa = 3.13NRR629 pKa = 11.84NGTWTAYY636 pKa = 10.16VADD639 pKa = 6.03LIPGQEE645 pKa = 4.1TGSPAGMPGSVQEE658 pKa = 4.34NPAIAFGGVDD668 pKa = 5.43GPTVWQDD675 pKa = 3.23DD676 pKa = 4.13RR677 pKa = 11.84NGSFDD682 pKa = 4.18IYY684 pKa = 10.27WSNAGTNEE692 pKa = 3.94MPVCIDD698 pKa = 4.28AGDD701 pKa = 3.76QQFPAIDD708 pKa = 3.87GLSVVWQDD716 pKa = 3.29TRR718 pKa = 11.84NGSSDD723 pKa = 3.02IYY725 pKa = 11.12LGDD728 pKa = 4.16LEE730 pKa = 4.79TGNRR734 pKa = 11.84TQITDD739 pKa = 3.48DD740 pKa = 4.47PADD743 pKa = 3.36QVYY746 pKa = 10.48PDD748 pKa = 3.74ISGDD752 pKa = 3.76FIVWQDD758 pKa = 3.36NRR760 pKa = 11.84NGNWDD765 pKa = 3.06IFLYY769 pKa = 10.79NRR771 pKa = 11.84VDD773 pKa = 3.36GTLVQVTDD781 pKa = 4.54DD782 pKa = 4.67PADD785 pKa = 3.42QIYY788 pKa = 9.96PSIAGNYY795 pKa = 9.11IVWQDD800 pKa = 3.33NRR802 pKa = 11.84SGNWDD807 pKa = 3.21IFLYY811 pKa = 10.74AIDD814 pKa = 4.51GVLPASPAEE823 pKa = 4.1TGGMPEE829 pKa = 4.64PGLPPLAANLTANVTNGTAPLTVLFNATTSGDD861 pKa = 3.44PTAWSWEE868 pKa = 4.17FGDD871 pKa = 4.31GATATGEE878 pKa = 4.1GSVQYY883 pKa = 9.81TYY885 pKa = 11.04QSPGTYY891 pKa = 7.74TARR894 pKa = 11.84LTVTNEE900 pKa = 3.72TGGNATDD907 pKa = 3.8TVIVTVTQEE916 pKa = 3.68PAEE919 pKa = 4.16NVTDD923 pKa = 3.8NQTPEE928 pKa = 3.56AALRR932 pKa = 11.84ADD934 pKa = 4.19PEE936 pKa = 4.43TGAVPLTVTFQDD948 pKa = 3.37QSTGSIDD955 pKa = 2.77EE956 pKa = 4.3WLWFFGDD963 pKa = 4.12GNASASPNATHH974 pKa = 7.16TYY976 pKa = 10.3VEE978 pKa = 4.36AGNYY982 pKa = 7.36TVTLTVSGPAGSDD995 pKa = 3.25TANGTIAVLPAIPAPEE1011 pKa = 3.79EE1012 pKa = 3.78AAGNLARR1019 pKa = 11.84AA1020 pKa = 4.07

Molecular weight: 107.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A498GWT7|A0A498GWT7_9EURY Uncharacterized protein OS=Methanoculleus taiwanensis OX=1550565 GN=ABH15_12600 PE=4 SV=1
MM1 pKa = 7.68PAKK4 pKa = 9.89QGRR7 pKa = 11.84DD8 pKa = 2.86IVQLRR13 pKa = 11.84FRR15 pKa = 11.84DD16 pKa = 3.82PGIAAPLTPARR27 pKa = 11.84PDD29 pKa = 3.43DD30 pKa = 3.89VPEE33 pKa = 4.23RR34 pKa = 11.84NAMPFEE40 pKa = 4.23YY41 pKa = 10.83GNLSGVWKK49 pKa = 10.28IAGIPKK55 pKa = 9.76RR56 pKa = 11.84SAIMRR61 pKa = 11.84QNWFCRR67 pKa = 11.84CRR69 pKa = 3.39

Molecular weight: 7.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2433

0

2433

704774

41

2405

289.7

31.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.955 ± 0.064

1.298 ± 0.023

5.631 ± 0.04

7.005 ± 0.054

3.651 ± 0.036

8.428 ± 0.056

1.945 ± 0.022

6.684 ± 0.042

3.444 ± 0.047

9.575 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.519 ± 0.026

2.715 ± 0.034

4.729 ± 0.034

2.537 ± 0.025

6.716 ± 0.054

5.332 ± 0.031

5.78 ± 0.046

8.015 ± 0.043

0.985 ± 0.02

3.058 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski