Mycobacterium phage Argie

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482JG61|A0A482JG61_9CAUD Uncharacterized protein OS=Mycobacterium phage Argie OX=2517937 GN=89 PE=4 SV=1
MM1 pKa = 7.78PSYY4 pKa = 10.17TVTWEE9 pKa = 3.87IDD11 pKa = 3.46CANADD16 pKa = 4.02DD17 pKa = 4.4MLAAAEE23 pKa = 4.36FARR26 pKa = 11.84EE27 pKa = 3.98TQMATGTTAVVFTVTDD43 pKa = 3.68RR44 pKa = 11.84DD45 pKa = 3.76TGEE48 pKa = 4.08SAEE51 pKa = 4.08VDD53 pKa = 3.13LDD55 pKa = 4.87RR56 pKa = 11.84EE57 pKa = 4.58VEE59 pKa = 4.12CCRR62 pKa = 11.84DD63 pKa = 3.45CGRR66 pKa = 11.84VTRR69 pKa = 11.84VDD71 pKa = 4.23DD72 pKa = 4.27EE73 pKa = 4.62PVDD76 pKa = 3.42TDD78 pKa = 4.04AADD81 pKa = 4.66GEE83 pKa = 4.41DD84 pKa = 5.09GYY86 pKa = 11.47CGSCADD92 pKa = 3.9RR93 pKa = 11.84LYY95 pKa = 11.25EE96 pKa = 4.05EE97 pKa = 4.83VAAEE101 pKa = 4.03DD102 pKa = 3.37AALVYY107 pKa = 10.46DD108 pKa = 4.22EE109 pKa = 5.51HH110 pKa = 8.18CAICAEE116 pKa = 3.96RR117 pKa = 11.84RR118 pKa = 11.84GGNGG122 pKa = 2.82

Molecular weight: 13.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482JGL1|A0A482JGL1_9CAUD Minor tail protein OS=Mycobacterium phage Argie OX=2517937 GN=24 PE=4 SV=1
MM1 pKa = 7.17NPKK4 pKa = 9.59HH5 pKa = 6.33SKK7 pKa = 7.3WRR9 pKa = 11.84IWMRR13 pKa = 11.84HH14 pKa = 3.77SRR16 pKa = 11.84VVKK19 pKa = 10.34SGSPPLYY26 pKa = 10.59RR27 pKa = 11.84MLTFGMLCSGIAQAFDD43 pKa = 3.57RR44 pKa = 11.84EE45 pKa = 4.45APLSISSVTPTWFDD59 pKa = 3.16VVFIVWTILGALVVLVALYY78 pKa = 7.66MTDD81 pKa = 2.89EE82 pKa = 4.63SKK84 pKa = 10.79FHH86 pKa = 6.63ARR88 pKa = 11.84HH89 pKa = 6.27LGDD92 pKa = 3.81SLNLEE97 pKa = 4.57WIGLSMMLTCITVNTAAVFFFYY119 pKa = 10.62DD120 pKa = 3.97RR121 pKa = 11.84PPTSGGSWFQIMFAVWGCIRR141 pKa = 11.84LRR143 pKa = 11.84DD144 pKa = 3.29IRR146 pKa = 11.84RR147 pKa = 11.84AVRR150 pKa = 11.84QLQKK154 pKa = 11.18

Molecular weight: 17.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

19570

45

1835

212.7

23.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.947 ± 0.479

1.288 ± 0.196

6.326 ± 0.249

7.072 ± 0.305

2.453 ± 0.115

8.763 ± 0.475

1.732 ± 0.13

3.955 ± 0.232

3.117 ± 0.313

8.186 ± 0.303

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.351 ± 0.13

2.683 ± 0.16

5.692 ± 0.274

3.592 ± 0.168

6.919 ± 0.357

5.749 ± 0.2

6.045 ± 0.241

7.951 ± 0.306

2.024 ± 0.17

2.156 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski