Inoviridae sp. ctBZ32

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; unclassified Inoviridae

Average proteome isoelectric point is 7.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WB72|A0A5Q2WB72_9VIRU Head virion protein OS=Inoviridae sp. ctBZ32 OX=2656729 PE=4 SV=1
MM1 pKa = 7.14NRR3 pKa = 11.84DD4 pKa = 3.12RR5 pKa = 11.84VVLRR9 pKa = 11.84RR10 pKa = 11.84SSGGSVAHH18 pKa = 6.23LQRR21 pKa = 11.84VARR24 pKa = 11.84ALVGFIIGVCFAYY37 pKa = 10.32AAIVPAYY44 pKa = 9.03AQSIPATLNTQPSTPGVDD62 pKa = 3.31GFKK65 pKa = 10.9GGGVPNVQCRR75 pKa = 11.84TNKK78 pKa = 9.91YY79 pKa = 7.88QACIDD84 pKa = 3.73ADD86 pKa = 4.27SYY88 pKa = 11.57IGWTTLCFDD97 pKa = 4.31GGTVCRR103 pKa = 11.84NASGFTGDD111 pKa = 4.04RR112 pKa = 11.84TTQACEE118 pKa = 3.48TWRR121 pKa = 11.84GAVVTLTNCTTEE133 pKa = 4.16PSCPAGYY140 pKa = 8.14TLNGTTCQQYY150 pKa = 8.56TCPAGYY156 pKa = 8.19TLNGTMCDD164 pKa = 4.42PEE166 pKa = 4.9PEE168 pKa = 4.42PCPEE172 pKa = 3.85VGSFRR177 pKa = 11.84FDD179 pKa = 3.66NILTPRR185 pKa = 11.84TPVPNQAWPDD195 pKa = 3.42ARR197 pKa = 11.84TTCVDD202 pKa = 2.82GCLAMLRR209 pKa = 11.84AVSATPNAWVWSVQYY224 pKa = 11.11VDD226 pKa = 5.09GVGNPTGQCTGGISVGEE243 pKa = 4.32GPLGPDD249 pKa = 3.47TDD251 pKa = 4.17PATPEE256 pKa = 4.6DD257 pKa = 4.88DD258 pKa = 3.84PVEE261 pKa = 4.4PPTPEE266 pKa = 3.83DD267 pKa = 3.32AACIRR272 pKa = 11.84QGKK275 pKa = 8.89CPGVVNGQAVCMPCTSQPGGGSSTTTSGTTTNPDD309 pKa = 2.92GTTSTTEE316 pKa = 3.46RR317 pKa = 11.84RR318 pKa = 11.84TTTTCDD324 pKa = 2.84GGQCTTTTTTTTTTRR339 pKa = 11.84DD340 pKa = 3.42AQGNPTGTTTSTEE353 pKa = 4.03SRR355 pKa = 11.84TGPGTGPGSGGNGDD369 pKa = 3.37GDD371 pKa = 3.67GDD373 pKa = 3.82EE374 pKa = 5.0RR375 pKa = 11.84GSSFGGACAAGFACEE390 pKa = 4.86GDD392 pKa = 4.35AIQCAIARR400 pKa = 11.84EE401 pKa = 3.98QHH403 pKa = 5.93IKK405 pKa = 10.05NCRR408 pKa = 11.84FWEE411 pKa = 4.48SKK413 pKa = 10.84GGLSEE418 pKa = 4.32ADD420 pKa = 3.06AVKK423 pKa = 10.82AFDD426 pKa = 4.41DD427 pKa = 5.26ARR429 pKa = 11.84GTDD432 pKa = 3.41AKK434 pKa = 10.55KK435 pKa = 10.97LNAEE439 pKa = 3.85PVAFQNLEE447 pKa = 3.75RR448 pKa = 11.84TAFLGAGTLSDD459 pKa = 3.84VSFTVQGQTLSIPFSEE475 pKa = 4.88LVPFLQAMGLAFMAVCGVIAIGIIRR500 pKa = 11.84TGVAA504 pKa = 3.16

Molecular weight: 51.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W8R7|A0A5Q2W8R7_9VIRU Capsid protein G8P OS=Inoviridae sp. ctBZ32 OX=2656729 PE=3 SV=1
MM1 pKa = 7.59PVGLPHH7 pKa = 7.53DD8 pKa = 4.21GRR10 pKa = 11.84RR11 pKa = 11.84GRR13 pKa = 11.84AGLSRR18 pKa = 11.84ARR20 pKa = 11.84SARR23 pKa = 11.84AVTVNRR29 pKa = 11.84GNQAGGTRR37 pKa = 11.84RR38 pKa = 11.84TSGLRR43 pKa = 3.26

Molecular weight: 4.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1469

43

504

183.6

19.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.164 ± 0.987

2.451 ± 1.047

4.084 ± 0.617

3.812 ± 0.659

3.268 ± 0.2

10.211 ± 1.269

1.77 ± 0.747

3.676 ± 0.629

2.655 ± 0.588

7.624 ± 1.854

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.974 ± 0.428

1.974 ± 0.728

7.624 ± 0.983

4.152 ± 0.422

7.148 ± 1.251

5.718 ± 0.461

8.169 ± 2.121

9.258 ± 1.187

1.838 ± 0.569

1.43 ± 0.318
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski