Tobacco virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus; unclassified Polerovirus

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V0JHW2|A0A1V0JHW2_9LUTE RNA-directed RNA polymerase OS=Tobacco virus 2 OX=1972995 PE=4 SV=1
MM1 pKa = 7.23LTIQARR7 pKa = 11.84EE8 pKa = 3.85NDD10 pKa = 3.67DD11 pKa = 3.94QIILGSLGSQRR22 pKa = 11.84MKK24 pKa = 10.93YY25 pKa = 9.9IEE27 pKa = 5.51DD28 pKa = 3.36EE29 pKa = 4.18NQNYY33 pKa = 8.21TKK35 pKa = 10.51FSSEE39 pKa = 4.59YY40 pKa = 8.59YY41 pKa = 9.92SQSSMQAVPMYY52 pKa = 9.89YY53 pKa = 10.3FNVPKK58 pKa = 10.36GQWSVDD64 pKa = 3.39ISCEE68 pKa = 4.18GYY70 pKa = 10.21QPTSSTSDD78 pKa = 3.55PNRR81 pKa = 11.84GRR83 pKa = 11.84SDD85 pKa = 3.4GMIAYY90 pKa = 10.35SNADD94 pKa = 2.79SDD96 pKa = 4.02YY97 pKa = 9.5WNVGEE102 pKa = 4.53ADD104 pKa = 3.49GVKK107 pKa = 9.88ISKK110 pKa = 9.59LRR112 pKa = 11.84NDD114 pKa = 2.86NTYY117 pKa = 10.53RR118 pKa = 11.84QGHH121 pKa = 6.79PEE123 pKa = 4.05LEE125 pKa = 4.1INSCHH130 pKa = 5.84FRR132 pKa = 11.84EE133 pKa = 4.68GQLLEE138 pKa = 4.26RR139 pKa = 11.84DD140 pKa = 3.63ATISFHH146 pKa = 8.31VEE148 pKa = 3.32APTDD152 pKa = 3.4GRR154 pKa = 11.84FFLVGPAIQKK164 pKa = 5.49TAKK167 pKa = 9.33YY168 pKa = 9.73NYY170 pKa = 8.05TISYY174 pKa = 10.17GDD176 pKa = 3.06WTDD179 pKa = 3.7RR180 pKa = 11.84DD181 pKa = 3.91MEE183 pKa = 4.99LGLITVVLDD192 pKa = 3.29EE193 pKa = 4.77HH194 pKa = 8.61LEE196 pKa = 4.34GTGSANRR203 pKa = 11.84VRR205 pKa = 11.84RR206 pKa = 11.84PPRR209 pKa = 11.84EE210 pKa = 3.32GHH212 pKa = 6.13TYY214 pKa = 8.54MASPHH219 pKa = 5.77EE220 pKa = 4.38PEE222 pKa = 4.9GKK224 pKa = 9.47PVGNKK229 pKa = 9.3PRR231 pKa = 11.84DD232 pKa = 3.45EE233 pKa = 4.47TPIQTQEE240 pKa = 4.03RR241 pKa = 11.84QPDD244 pKa = 3.65QTPSDD249 pKa = 4.17DD250 pKa = 3.7VSDD253 pKa = 4.08AGSVNSGGPTEE264 pKa = 4.13SLRR267 pKa = 11.84LEE269 pKa = 4.24FGVNSDD275 pKa = 3.13STYY278 pKa = 11.27DD279 pKa = 3.5ATVDD283 pKa = 3.44GTDD286 pKa = 3.38WPRR289 pKa = 11.84IPPPRR294 pKa = 11.84HH295 pKa = 5.24PPEE298 pKa = 4.29PRR300 pKa = 11.84VSGNSRR306 pKa = 11.84TVTDD310 pKa = 5.0FSSKK314 pKa = 11.18ADD316 pKa = 4.0LLEE319 pKa = 4.27NWDD322 pKa = 4.58AEE324 pKa = 4.39HH325 pKa = 7.23FDD327 pKa = 3.6PGYY330 pKa = 10.6SKK332 pKa = 11.2EE333 pKa = 4.24DD334 pKa = 3.24VAAATIIAHH343 pKa = 6.99GSIQDD348 pKa = 3.53GRR350 pKa = 11.84SMLEE354 pKa = 3.66KK355 pKa = 10.35RR356 pKa = 11.84EE357 pKa = 4.03EE358 pKa = 3.98NVKK361 pKa = 10.85NKK363 pKa = 8.99TSSWKK368 pKa = 9.93PPSLKK373 pKa = 10.48AVSPAIAKK381 pKa = 9.51LRR383 pKa = 11.84SIRR386 pKa = 11.84KK387 pKa = 7.69SQPLEE392 pKa = 4.04GGTLNKK398 pKa = 10.38DD399 pKa = 3.0ATDD402 pKa = 4.12GVSSIGSGSLTGGTLKK418 pKa = 10.59RR419 pKa = 11.84KK420 pKa = 8.53ATIEE424 pKa = 3.84EE425 pKa = 4.38RR426 pKa = 11.84LLQTLTTEE434 pKa = 3.67QRR436 pKa = 11.84LWYY439 pKa = 10.26EE440 pKa = 4.08NFKK443 pKa = 9.32KK444 pKa = 9.31TNPPAATQWLFEE456 pKa = 4.28YY457 pKa = 10.49QPPPQVDD464 pKa = 3.24RR465 pKa = 11.84NIAEE469 pKa = 4.33KK470 pKa = 10.39PFQGRR475 pKa = 11.84KK476 pKa = 8.39

Molecular weight: 53.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V0JHV8|A0A1V0JHV8_9LUTE Movement protein OS=Tobacco virus 2 OX=1972995 PE=3 SV=1
MM1 pKa = 6.72STVVVKK7 pKa = 10.83GNVNGGVQQPRR18 pKa = 11.84MRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84QSLRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84ANRR31 pKa = 11.84VQPVVMVTAPGQPRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84GGNRR55 pKa = 11.84RR56 pKa = 11.84SRR58 pKa = 11.84RR59 pKa = 11.84TGVPRR64 pKa = 11.84GRR66 pKa = 11.84GSSEE70 pKa = 3.73TFVFTKK76 pKa = 10.79DD77 pKa = 3.28NLVGNTQGSFTFGPSLSDD95 pKa = 3.42CPAFKK100 pKa = 10.76DD101 pKa = 4.47GILKK105 pKa = 10.29AYY107 pKa = 9.81HH108 pKa = 6.59EE109 pKa = 4.33YY110 pKa = 10.69KK111 pKa = 9.21ITSILLQFVSEE122 pKa = 4.33ASSTSSGSIAYY133 pKa = 9.43EE134 pKa = 3.86LDD136 pKa = 3.29PHH138 pKa = 6.86CKK140 pKa = 9.78VSSLQSYY147 pKa = 7.92VNKK150 pKa = 10.02FQITKK155 pKa = 10.26GGAKK159 pKa = 9.43TYY161 pKa = 7.27QARR164 pKa = 11.84MINGVEE170 pKa = 3.88WHH172 pKa = 7.28DD173 pKa = 4.24SSEE176 pKa = 4.13DD177 pKa = 3.25QCRR180 pKa = 11.84ILWKK184 pKa = 10.94GNGKK188 pKa = 10.24SSDD191 pKa = 3.53SAGSFRR197 pKa = 11.84VTIKK201 pKa = 10.78VALQNPKK208 pKa = 10.46

Molecular weight: 23.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2039

156

636

339.8

37.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.357 ± 0.903

1.667 ± 0.379

3.973 ± 0.863

6.376 ± 0.801

3.58 ± 0.4

6.719 ± 0.559

1.913 ± 0.283

4.708 ± 0.462

6.13 ± 0.811

8.092 ± 1.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.962 ± 0.27

4.414 ± 0.277

6.229 ± 0.718

4.169 ± 0.419

5.885 ± 0.982

9.564 ± 0.661

6.278 ± 0.37

5.64 ± 0.406

1.717 ± 0.119

3.629 ± 0.289
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski