Radi vesiculovirus
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D3R1W3|A0A0D3R1W3_9RHAB Glycoprotein OS=Radi vesiculovirus OX=1972566 PE=3 SV=1
MM1 pKa = 8.08 DD2 pKa = 4.74 LQRR5 pKa = 11.84 SAFEE9 pKa = 4.05 ALRR12 pKa = 11.84 NYY14 pKa = 9.87 PNLEE18 pKa = 3.93 NALADD23 pKa = 3.91 ADD25 pKa = 4.0 EE26 pKa = 4.91 SEE28 pKa = 4.64 SLRR31 pKa = 11.84 DD32 pKa = 3.57 PPSPNSSQAHH42 pKa = 4.98 EE43 pKa = 4.35 SEE45 pKa = 4.41 GEE47 pKa = 3.99 KK48 pKa = 10.52 FYY50 pKa = 10.64 LTDD53 pKa = 3.69 QLDD56 pKa = 3.89 LLEE59 pKa = 5.38 SSDD62 pKa = 5.26 EE63 pKa = 4.04 EE64 pKa = 5.69 DD65 pKa = 3.85 EE66 pKa = 5.63 DD67 pKa = 4.79 LSTDD71 pKa = 3.11 IGSSGQHH78 pKa = 5.45 QDD80 pKa = 2.76 TYY82 pKa = 11.9 SNIDD86 pKa = 3.7 DD87 pKa = 4.19 RR88 pKa = 11.84 SASNQDD94 pKa = 2.84 EE95 pKa = 4.31 DD96 pKa = 4.64 SEE98 pKa = 4.67 EE99 pKa = 3.77 EE100 pKa = 3.94 RR101 pKa = 11.84 EE102 pKa = 4.25 AYY104 pKa = 9.6 GVHH107 pKa = 6.36 FPSEE111 pKa = 4.36 KK112 pKa = 9.28 PTIDD116 pKa = 5.23 LSDD119 pKa = 4.22 LPATLQRR126 pKa = 11.84 RR127 pKa = 11.84 IFVDD131 pKa = 3.49 ISNAFKK137 pKa = 11.27 KK138 pKa = 10.28 MMEE141 pKa = 4.44 APSCQHH147 pKa = 5.7 FPVMVRR153 pKa = 11.84 GRR155 pKa = 11.84 IEE157 pKa = 5.33 LIPSSDD163 pKa = 3.39 MAIHH167 pKa = 5.26 QTPSSATTPEE177 pKa = 4.43 SEE179 pKa = 5.14 DD180 pKa = 3.99 LPPSPVKK187 pKa = 10.12 RR188 pKa = 11.84 DD189 pKa = 3.33 RR190 pKa = 11.84 LLDD193 pKa = 3.35 RR194 pKa = 11.84 FKK196 pKa = 10.27 IHH198 pKa = 6.7 HH199 pKa = 7.21 RR200 pKa = 11.84 STQDD204 pKa = 3.31 PLEE207 pKa = 4.23 TSFVEE212 pKa = 4.13 IFGSEE217 pKa = 3.94 DD218 pKa = 2.96 SARR221 pKa = 11.84 AAWGDD226 pKa = 3.26 GSLSYY231 pKa = 10.97 KK232 pKa = 10.29 EE233 pKa = 4.65 LLILGLRR240 pKa = 11.84 RR241 pKa = 11.84 KK242 pKa = 10.1 GVFNRR247 pKa = 11.84 MRR249 pKa = 11.84 IAYY252 pKa = 8.71 DD253 pKa = 3.25 LTPIFEE259 pKa = 4.36
Molecular weight: 29.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.546
IPC2_protein 4.431
IPC_protein 4.393
Toseland 4.228
ProMoST 4.482
Dawson 4.355
Bjellqvist 4.52
Wikipedia 4.228
Rodwell 4.228
Grimsley 4.139
Solomon 4.342
Lehninger 4.304
Nozaki 4.457
DTASelect 4.635
Thurlkill 4.228
EMBOSS 4.253
Sillero 4.507
Patrickios 3.986
IPC_peptide 4.355
IPC2_peptide 4.495
IPC2.peptide.svr19 4.434
Protein with the highest isoelectric point:
>tr|A0A0D3R1T8|A0A0D3R1T8_9RHAB Phosphoprotein OS=Radi vesiculovirus OX=1972566 PE=3 SV=1
MM1 pKa = 7.6 KK2 pKa = 10.18 GFKK5 pKa = 10.85 AMFSKK10 pKa = 10.63 KK11 pKa = 9.62 KK12 pKa = 9.46 SKK14 pKa = 10.03 KK15 pKa = 9.51 GKK17 pKa = 9.14 EE18 pKa = 3.62 IKK20 pKa = 10.62 AKK22 pKa = 10.84 GKK24 pKa = 10.87 GKK26 pKa = 10.24 DD27 pKa = 3.16 KK28 pKa = 11.02 NKK30 pKa = 10.43 QYY32 pKa = 11.24 DD33 pKa = 3.59 EE34 pKa = 4.7 DD35 pKa = 4.2 EE36 pKa = 4.53 RR37 pKa = 11.84 PPAYY41 pKa = 10.55 FNVAPEE47 pKa = 4.24 WGSASAPMFGYY58 pKa = 10.44 DD59 pKa = 4.53 EE60 pKa = 4.98 YY61 pKa = 11.02 IEE63 pKa = 4.43 SQSSIQSLKK72 pKa = 9.45 IQFRR76 pKa = 11.84 YY77 pKa = 9.95 SLEE80 pKa = 4.06 VTVDD84 pKa = 3.3 YY85 pKa = 10.75 PFANFFEE92 pKa = 5.04 ALQSMRR98 pKa = 11.84 NWKK101 pKa = 9.38 IDD103 pKa = 3.26 YY104 pKa = 9.19 TGYY107 pKa = 10.33 QNKK110 pKa = 9.82 KK111 pKa = 9.15 SIYY114 pKa = 8.97 NIIMMYY120 pKa = 8.01 ATKK123 pKa = 10.43 RR124 pKa = 11.84 LRR126 pKa = 11.84 AGPRR130 pKa = 11.84 GLQGRR135 pKa = 11.84 KK136 pKa = 5.82 STEE139 pKa = 3.63 YY140 pKa = 10.09 SAEE143 pKa = 3.73 GSGRR147 pKa = 11.84 YY148 pKa = 6.75 TLHH151 pKa = 7.24 HH152 pKa = 7.3 DD153 pKa = 3.92 LGDD156 pKa = 3.64 LSGMRR161 pKa = 11.84 FAEE164 pKa = 4.15 EE165 pKa = 3.84 RR166 pKa = 11.84 FVRR169 pKa = 11.84 TWQDD173 pKa = 2.89 QLRR176 pKa = 11.84 RR177 pKa = 11.84 GTINFYY183 pKa = 10.44 VWLGEE188 pKa = 3.94 TDD190 pKa = 4.28 DD191 pKa = 4.77 NSNLPPLICPGDD203 pKa = 3.6 FKK205 pKa = 11.28 DD206 pKa = 3.67 ASEE209 pKa = 4.75 FNMSCEE215 pKa = 3.64 IMGIRR220 pKa = 11.84 VEE222 pKa = 4.19 VQPDD226 pKa = 3.56 NQWVVKK232 pKa = 9.65 EE233 pKa = 4.01 AA234 pKa = 3.52
Molecular weight: 27.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.335
IPC2_protein 8.331
IPC_protein 8.273
Toseland 8.843
ProMoST 8.726
Dawson 9.165
Bjellqvist 8.975
Wikipedia 9.341
Rodwell 9.326
Grimsley 9.209
Solomon 9.268
Lehninger 9.224
Nozaki 9.004
DTASelect 8.916
Thurlkill 9.019
EMBOSS 9.297
Sillero 9.165
Patrickios 4.546
IPC_peptide 9.253
IPC2_peptide 7.571
IPC2.peptide.svr19 7.606
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3577
234
2100
715.4
81.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.284 ± 0.883
1.594 ± 0.241
6.039 ± 0.616
5.927 ± 0.58
4.389 ± 0.214
6.458 ± 0.443
2.572 ± 0.23
6.458 ± 0.651
5.563 ± 0.624
9.785 ± 1.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.544 ± 0.152
4.166 ± 0.381
4.501 ± 0.446
3.327 ± 0.342
6.346 ± 0.589
9.365 ± 0.483
5.088 ± 0.436
5.396 ± 0.593
2.041 ± 0.269
3.159 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here