Parahaliea aestuarii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Halieaceae; Parahaliea

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3815 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C9A0W4|A0A5C9A0W4_9GAMM LysR family transcriptional regulator OS=Parahaliea aestuarii OX=1852021 GN=FVW59_05175 PE=3 SV=1
MM1 pKa = 7.58NLQNYY6 pKa = 9.06NLDD9 pKa = 2.85IGYY12 pKa = 10.46ALGNGMVLRR21 pKa = 11.84SITGYY26 pKa = 11.01NDD28 pKa = 3.06TDD30 pKa = 4.2LEE32 pKa = 4.39ITSVPSAADD41 pKa = 3.15VFFRR45 pKa = 11.84QDD47 pKa = 2.5NRR49 pKa = 11.84MDD51 pKa = 3.9GDD53 pKa = 3.95FMQEE57 pKa = 3.87FRR59 pKa = 11.84LEE61 pKa = 4.17MNEE64 pKa = 4.15EE65 pKa = 3.97IAGLTGVASLFYY77 pKa = 11.03GDD79 pKa = 5.0FDD81 pKa = 3.65QHH83 pKa = 7.06SEE85 pKa = 4.22SYY87 pKa = 10.07ILYY90 pKa = 8.27YY91 pKa = 10.25QAFPVQDD98 pKa = 3.49GTFDD102 pKa = 3.53NSTEE106 pKa = 3.59TWAAYY111 pKa = 10.5ADD113 pKa = 3.55FRR115 pKa = 11.84YY116 pKa = 10.35RR117 pKa = 11.84LTDD120 pKa = 3.37SLSLLFGGRR129 pKa = 11.84YY130 pKa = 9.16QDD132 pKa = 3.22DD133 pKa = 4.06TVRR136 pKa = 11.84NQADD140 pKa = 3.63VEE142 pKa = 4.51SALSGGSQYY151 pKa = 11.38DD152 pKa = 3.81LEE154 pKa = 5.05SGFDD158 pKa = 3.38MFLPKK163 pKa = 10.48AGLSLDD169 pKa = 3.23ITDD172 pKa = 4.19NQTVAATASRR182 pKa = 11.84GYY184 pKa = 9.97RR185 pKa = 11.84QGFAEE190 pKa = 4.13RR191 pKa = 11.84RR192 pKa = 11.84LGSEE196 pKa = 3.87AGLVDD201 pKa = 3.98VDD203 pKa = 5.11PEE205 pKa = 4.35FVWAYY210 pKa = 9.09EE211 pKa = 3.45IAYY214 pKa = 9.93RR215 pKa = 11.84ISALNNRR222 pKa = 11.84LLLGANVFYY231 pKa = 11.11NDD233 pKa = 3.67YY234 pKa = 9.64TDD236 pKa = 3.55QQITVTNPDD245 pKa = 3.89FAPLTNTMNAGDD257 pKa = 4.14SEE259 pKa = 4.69SWGAEE264 pKa = 3.35IEE266 pKa = 4.21AQLAFDD272 pKa = 3.9NGLRR276 pKa = 11.84IYY278 pKa = 10.92GSLGLLEE285 pKa = 4.58TEE287 pKa = 4.99LGDD290 pKa = 6.04FPAEE294 pKa = 4.14DD295 pKa = 4.7CSNGSCDD302 pKa = 3.21GNQYY306 pKa = 10.85SEE308 pKa = 4.51APEE311 pKa = 4.08VTASLGGEE319 pKa = 4.01YY320 pKa = 10.18RR321 pKa = 11.84HH322 pKa = 7.04DD323 pKa = 3.6SGFFASLAASYY334 pKa = 10.2TDD336 pKa = 2.99SFYY339 pKa = 11.22RR340 pKa = 11.84SVDD343 pKa = 3.32NADD346 pKa = 3.82DD347 pKa = 4.75LEE349 pKa = 4.94VDD351 pKa = 3.45SSFVVDD357 pKa = 4.84AKK359 pKa = 10.43IGYY362 pKa = 9.4DD363 pKa = 3.36FGHH366 pKa = 6.25FRR368 pKa = 11.84VSAYY372 pKa = 10.57ANNVFDD378 pKa = 4.78EE379 pKa = 4.97EE380 pKa = 4.88YY381 pKa = 10.06LTGISNANSAFIGDD395 pKa = 3.9PRR397 pKa = 11.84AMGVEE402 pKa = 4.2VTAAFF407 pKa = 3.91

Molecular weight: 44.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C8ZY70|A0A5C8ZY70_9GAMM Type I secretion system permease/ATPase OS=Parahaliea aestuarii OX=1852021 GN=FVW59_06940 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.52RR12 pKa = 11.84KK13 pKa = 8.26RR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.24NGRR28 pKa = 11.84LVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.02GRR39 pKa = 11.84KK40 pKa = 8.77RR41 pKa = 11.84LAAA44 pKa = 4.42

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3815

0

3815

1363449

28

3668

357.4

39.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.236 ± 0.043

1.054 ± 0.014

5.941 ± 0.03

6.245 ± 0.035

3.609 ± 0.025

8.371 ± 0.043

2.134 ± 0.017

4.577 ± 0.029

2.688 ± 0.032

10.908 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.264 ± 0.021

3.007 ± 0.026

4.873 ± 0.027

4.07 ± 0.03

6.815 ± 0.039

5.986 ± 0.031

4.869 ± 0.029

7.146 ± 0.034

1.41 ± 0.016

2.797 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski