Cochliobolus sativus (strain ND90Pr / ATCC 201652) (Common root rot and spot blotch fungus) (Bipolaris sorokiniana)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Bipolaris; Bipolaris sorokiniana

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12174 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M2SVJ7|M2SVJ7_COCSN Fatty acid hydroxylase domain-containing protein OS=Cochliobolus sativus (strain ND90Pr / ATCC 201652) OX=665912 GN=COCSADRAFT_163448 PE=4 SV=1
MM1 pKa = 8.05DD2 pKa = 4.72LHH4 pKa = 8.59SDD6 pKa = 4.46DD7 pKa = 6.4GILGYY12 pKa = 10.81NDD14 pKa = 3.75GDD16 pKa = 3.6IEE18 pKa = 5.83LDD20 pKa = 3.7LEE22 pKa = 4.6PPPSYY27 pKa = 11.26GPDD30 pKa = 3.49DD31 pKa = 4.39DD32 pKa = 5.92VSIGDD37 pKa = 3.66AASANGMDD45 pKa = 4.12AQTAPGGDD53 pKa = 3.5NDD55 pKa = 5.76DD56 pKa = 4.09YY57 pKa = 11.43MVDD60 pKa = 3.38QEE62 pKa = 5.34DD63 pKa = 4.47VIEE66 pKa = 4.47EE67 pKa = 4.35DD68 pKa = 4.75YY69 pKa = 11.23SYY71 pKa = 11.43QDD73 pKa = 2.8QDD75 pKa = 2.83VDD77 pKa = 3.47VDD79 pKa = 3.62VDD81 pKa = 3.59VDD83 pKa = 3.74VDD85 pKa = 3.52VDD87 pKa = 4.04ADD89 pKa = 3.98AEE91 pKa = 4.37QPAATDD97 pKa = 4.16TIATQAEE104 pKa = 4.54AEE106 pKa = 4.38VQDD109 pKa = 4.77EE110 pKa = 4.35DD111 pKa = 4.27LLDD114 pKa = 3.92YY115 pKa = 11.35SEE117 pKa = 5.71EE118 pKa = 4.48DD119 pKa = 4.21DD120 pKa = 5.79DD121 pKa = 4.72YY122 pKa = 11.79HH123 pKa = 6.71DD124 pKa = 3.83TALRR128 pKa = 11.84SPWLQQHH135 pKa = 7.22DD136 pKa = 4.29SMQDD140 pKa = 3.04EE141 pKa = 5.07PGPQNEE147 pKa = 4.2PSQAQDD153 pKa = 3.21APVQQDD159 pKa = 4.46EE160 pKa = 4.91IPADD164 pKa = 4.0LQALMSTNTSKK175 pKa = 10.97NPQSPHH181 pKa = 5.08AHH183 pKa = 6.82PDD185 pKa = 3.33VVADD189 pKa = 5.09GSTKK193 pKa = 10.65GNNNEE198 pKa = 3.66NSPRR202 pKa = 11.84ARR204 pKa = 11.84SADD207 pKa = 3.62PNEE210 pKa = 4.47EE211 pKa = 3.95DD212 pKa = 4.06AQTNGDD218 pKa = 4.14DD219 pKa = 4.88GGITLKK225 pKa = 10.84DD226 pKa = 3.62QEE228 pKa = 4.45EE229 pKa = 4.32HH230 pKa = 6.7SSAEE234 pKa = 3.98SGHH237 pKa = 5.49NHH239 pKa = 5.76MEE241 pKa = 4.49PPSHH245 pKa = 6.08GHH247 pKa = 7.0DD248 pKa = 3.16EE249 pKa = 4.23SAQVDD254 pKa = 3.8EE255 pKa = 5.22HH256 pKa = 7.83EE257 pKa = 5.11EE258 pKa = 4.05DD259 pKa = 3.44TSFQQLPPVTVNCMGEE275 pKa = 4.2EE276 pKa = 3.65LWLFKK281 pKa = 10.76QHH283 pKa = 7.33DD284 pKa = 4.37YY285 pKa = 11.41EE286 pKa = 5.65NSGDD290 pKa = 3.59WLLADD295 pKa = 3.56VSVAKK300 pKa = 10.05TSMSDD305 pKa = 3.26LFQTCRR311 pKa = 11.84SALGHH316 pKa = 5.83EE317 pKa = 4.78VLNGMEE323 pKa = 3.48IGFRR327 pKa = 11.84FDD329 pKa = 3.73HH330 pKa = 5.78FQNMEE335 pKa = 4.12LFEE338 pKa = 5.04EE339 pKa = 4.35NTACVAISLEE349 pKa = 4.03RR350 pKa = 11.84LVGYY354 pKa = 7.91YY355 pKa = 9.9HH356 pKa = 7.65ALHH359 pKa = 6.29EE360 pKa = 4.22QDD362 pKa = 3.63

Molecular weight: 39.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M2TEI1|M2TEI1_COCSN TauD domain-containing protein (Fragment) OS=Cochliobolus sativus (strain ND90Pr / ATCC 201652) OX=665912 GN=COCSADRAFT_81895 PE=4 SV=1
MM1 pKa = 7.31LHH3 pKa = 6.5HH4 pKa = 7.21APHH7 pKa = 6.42TMHH10 pKa = 6.84ATLLSSLRR18 pKa = 11.84PSPPSSSPFLPRR30 pKa = 11.84AQRR33 pKa = 11.84IHH35 pKa = 6.15SLRR38 pKa = 11.84ARR40 pKa = 11.84TTSPRR45 pKa = 11.84THH47 pKa = 6.92LPLSFPSSAPLHH59 pKa = 4.87STIPKK64 pKa = 10.14LLLLHH69 pKa = 6.3PTLTIPLAPSTNPLWPRR86 pKa = 11.84LPLRR90 pKa = 11.84TPFPFPNPLSNVRR103 pKa = 11.84TMHH106 pKa = 7.33RR107 pKa = 11.84ITVV110 pKa = 3.42

Molecular weight: 12.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12174

0

12174

5418225

49

7203

445.1

49.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.695 ± 0.022

1.293 ± 0.009

5.569 ± 0.017

6.099 ± 0.022

3.678 ± 0.013

6.681 ± 0.022

2.474 ± 0.01

4.937 ± 0.015

5.124 ± 0.019

8.674 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.243 ± 0.009

3.788 ± 0.013

6.096 ± 0.024

4.176 ± 0.018

5.973 ± 0.02

8.094 ± 0.027

6.072 ± 0.016

6.018 ± 0.016

1.467 ± 0.008

2.848 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski