Frigoribacterium sp. RIT-PI-h

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Frigoribacterium; unclassified Frigoribacterium

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2815 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N1M1S2|A0A0N1M1S2_9MICO Histidyl-tRNA synthetase OS=Frigoribacterium sp. RIT-PI-h OX=1690245 GN=AEQ27_13795 PE=3 SV=1
MM1 pKa = 7.25IRR3 pKa = 11.84SSTALRR9 pKa = 11.84ALALAGAASVLLAACSTASPSDD31 pKa = 3.87DD32 pKa = 3.49ASSSSSAKK40 pKa = 10.03ADD42 pKa = 3.57PAAKK46 pKa = 10.19CEE48 pKa = 4.09VGTPSTDD55 pKa = 2.92PFAIGTMLPLTGTLAYY71 pKa = 10.17LGPPAIAGANLAISDD86 pKa = 3.69INAAGGVLDD95 pKa = 4.16QPAEE99 pKa = 4.07ISTSTDD105 pKa = 3.02SGDD108 pKa = 3.94SNDD111 pKa = 3.62MTVSSNAATEE121 pKa = 4.94LIDD124 pKa = 3.98AKK126 pKa = 11.2VPVVLGAEE134 pKa = 4.37SSSVTLNVVDD144 pKa = 5.0QLTSNCIIEE153 pKa = 4.26ISPANTASSLSGYY166 pKa = 9.56SSYY169 pKa = 11.47YY170 pKa = 10.15YY171 pKa = 9.43RR172 pKa = 11.84TAPPDD177 pKa = 3.69SVQGSALGQQITADD191 pKa = 3.53GNANVAFLVFNDD203 pKa = 4.01TYY205 pKa = 11.06GTGLRR210 pKa = 11.84DD211 pKa = 3.51STQKK215 pKa = 10.78SIEE218 pKa = 4.25SSGGTVVYY226 pKa = 10.57GGTGKK231 pKa = 10.98GEE233 pKa = 4.21EE234 pKa = 4.4FPPGQTTFSSEE245 pKa = 3.56VSAAIAAEE253 pKa = 3.84PDD255 pKa = 4.02AIVILAFDD263 pKa = 3.92EE264 pKa = 4.59TKK266 pKa = 10.85SIVPEE271 pKa = 4.35LKK273 pKa = 10.26SQGWDD278 pKa = 3.06MSKK281 pKa = 11.1VYY283 pKa = 10.34MSDD286 pKa = 3.16GNTADD291 pKa = 3.5YY292 pKa = 11.13SADD295 pKa = 3.69FEE297 pKa = 5.07PGTLEE302 pKa = 4.11GAKK305 pKa = 9.21GTIPGADD312 pKa = 3.07ASADD316 pKa = 3.76FKK318 pKa = 11.5SKK320 pKa = 10.55LVAGYY325 pKa = 8.86QASEE329 pKa = 4.14GGEE332 pKa = 3.91LADD335 pKa = 4.23FSYY338 pKa = 11.03AAEE341 pKa = 4.53SYY343 pKa = 11.03DD344 pKa = 4.52AVILTALAAQAGGGTDD360 pKa = 3.37AGTIQANMAAVSGANGGTEE379 pKa = 3.95CTTYY383 pKa = 11.36ADD385 pKa = 4.44CKK387 pKa = 9.2TALDD391 pKa = 4.24AGDD394 pKa = 5.21DD395 pKa = 3.38IHH397 pKa = 6.17YY398 pKa = 7.46TGPSGIGPFNDD409 pKa = 3.4NNDD412 pKa = 3.67PSSASIGVYY421 pKa = 9.69TFDD424 pKa = 4.74ADD426 pKa = 3.77NKK428 pKa = 10.01PVYY431 pKa = 8.96QTGIEE436 pKa = 4.33GKK438 pKa = 10.17VEE440 pKa = 3.79

Molecular weight: 44.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N0KW66|A0A0N0KW66_9MICO Transcriptional regulator OS=Frigoribacterium sp. RIT-PI-h OX=1690245 GN=AEQ27_12095 PE=4 SV=1
MM1 pKa = 7.94RR2 pKa = 11.84GPAPRR7 pKa = 11.84GRR9 pKa = 11.84RR10 pKa = 11.84GRR12 pKa = 11.84ARR14 pKa = 11.84PRR16 pKa = 11.84AASRR20 pKa = 11.84AAGNGAVLGRR30 pKa = 11.84TPRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84PRR37 pKa = 11.84RR38 pKa = 11.84PRR40 pKa = 11.84RR41 pKa = 11.84ASTRR45 pKa = 11.84RR46 pKa = 11.84DD47 pKa = 3.02RR48 pKa = 11.84SAPARR53 pKa = 11.84PSARR57 pKa = 11.84ARR59 pKa = 11.84TRR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84GRR65 pKa = 11.84PGGVRR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84AGRR75 pKa = 11.84SGPGRR80 pKa = 11.84GGAAAGGGWGSWSS93 pKa = 3.24

Molecular weight: 9.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2815

0

2815

842116

38

3016

299.2

31.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.168 ± 0.055

0.45 ± 0.01

6.652 ± 0.045

5.194 ± 0.042

3.032 ± 0.029

9.321 ± 0.043

1.912 ± 0.026

3.687 ± 0.037

1.946 ± 0.032

9.927 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.627 ± 0.019

1.756 ± 0.021

5.564 ± 0.036

2.77 ± 0.031

7.254 ± 0.054

5.974 ± 0.033

6.523 ± 0.054

9.976 ± 0.058

1.389 ± 0.019

1.879 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski