Limosilactobacillus secaliphilus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Limosilactobacillus

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1466 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R2HZA4|A0A0R2HZA4_9LACO Uncharacterized protein OS=Limosilactobacillus secaliphilus OX=396268 GN=IV45_GL000572 PE=4 SV=1
MM1 pKa = 7.23KK2 pKa = 10.38AVVVYY7 pKa = 8.11ATITGNNEE15 pKa = 3.5DD16 pKa = 3.74VADD19 pKa = 5.08IITDD23 pKa = 3.42ALEE26 pKa = 3.87EE27 pKa = 4.44HH28 pKa = 5.99NVEE31 pKa = 4.21VEE33 pKa = 3.87EE34 pKa = 5.01SEE36 pKa = 4.71ISTADD41 pKa = 3.24VADD44 pKa = 4.54FEE46 pKa = 4.67NADD49 pKa = 3.34ICIVCPYY56 pKa = 9.68TYY58 pKa = 10.69DD59 pKa = 3.37EE60 pKa = 4.91GALPDD65 pKa = 4.3EE66 pKa = 5.07GLDD69 pKa = 3.98FYY71 pKa = 11.87DD72 pKa = 5.66DD73 pKa = 4.08LKK75 pKa = 10.69EE76 pKa = 4.22TNLKK80 pKa = 9.94GKK82 pKa = 10.16VYY84 pKa = 10.22GVAGSGDD91 pKa = 3.54TYY93 pKa = 10.53YY94 pKa = 11.38AEE96 pKa = 4.62YY97 pKa = 11.18YY98 pKa = 10.55CMAVDD103 pKa = 4.15KK104 pKa = 10.66FGKK107 pKa = 10.52AFDD110 pKa = 3.58QTGATKK116 pKa = 10.37GAEE119 pKa = 3.83NVHH122 pKa = 6.46INLTPDD128 pKa = 3.17EE129 pKa = 5.2DD130 pKa = 4.19DD131 pKa = 4.18VKK133 pKa = 11.38KK134 pKa = 10.83LDD136 pKa = 3.74QFVNDD141 pKa = 4.71LLAKK145 pKa = 10.54AKK147 pKa = 10.48

Molecular weight: 16.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R2I0A0|A0A0R2I0A0_9LACO 50S ribosomal protein L24 OS=Limosilactobacillus secaliphilus OX=396268 GN=rplX PE=3 SV=1
MM1 pKa = 7.13QEE3 pKa = 3.51LMKK6 pKa = 10.31RR7 pKa = 11.84TFQPKK12 pKa = 8.02KK13 pKa = 7.4RR14 pKa = 11.84HH15 pKa = 5.36RR16 pKa = 11.84ARR18 pKa = 11.84VHH20 pKa = 5.72GFRR23 pKa = 11.84ARR25 pKa = 11.84MSTSNGRR32 pKa = 11.84KK33 pKa = 8.4VLARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.2KK41 pKa = 10.05GRR43 pKa = 11.84KK44 pKa = 8.66VLSAA48 pKa = 4.05

Molecular weight: 5.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1466

0

1466

480622

48

6533

327.8

36.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.249 ± 0.068

0.628 ± 0.018

6.254 ± 0.077

4.826 ± 0.063

4.044 ± 0.05

6.819 ± 0.07

2.79 ± 0.037

6.328 ± 0.066

6.279 ± 0.063

9.539 ± 0.089

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.816 ± 0.044

4.831 ± 0.06

3.871 ± 0.049

5.0 ± 0.071

4.356 ± 0.066

5.753 ± 0.057

5.627 ± 0.11

7.161 ± 0.043

1.161 ± 0.024

3.667 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski