Mycobacteriaceae bacterium 1482268.1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; unclassified Mycobacteriaceae

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4776 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A0PUA0|A0A1A0PUA0_9MYCO Uncharacterized protein OS=Mycobacteriaceae bacterium 1482268.1 OX=1834080 GN=A5662_07665 PE=4 SV=1
MM1 pKa = 8.17RR2 pKa = 11.84IPRR5 pKa = 11.84AAVALTAALLLGAVGCTNQIPGTAIQDD32 pKa = 3.59PTQPPLALSDD42 pKa = 3.76DD43 pKa = 4.37GYY45 pKa = 11.37GIVAGYY51 pKa = 9.49PDD53 pKa = 4.7APVQIEE59 pKa = 5.42LYY61 pKa = 9.95TEE63 pKa = 4.28PQCGHH68 pKa = 7.05CAQLQTAYY76 pKa = 10.28GADD79 pKa = 3.33IEE81 pKa = 4.69RR82 pKa = 11.84SINLGEE88 pKa = 4.06LAVTYY93 pKa = 10.47RR94 pKa = 11.84PLTFLDD100 pKa = 3.68SPSTHH105 pKa = 6.62EE106 pKa = 3.91YY107 pKa = 8.9SARR110 pKa = 11.84VANAMFLAVGAPEE123 pKa = 4.18EE124 pKa = 4.0TDD126 pKa = 3.52EE127 pKa = 6.42ADD129 pKa = 3.33MASGPEE135 pKa = 3.81FQRR138 pKa = 11.84FVEE141 pKa = 4.65DD142 pKa = 3.56LWGHH146 pKa = 4.89QQLGGPGPSDD156 pKa = 3.12AQMAKK161 pKa = 9.0MAEE164 pKa = 4.18DD165 pKa = 3.21SGMPADD171 pKa = 3.54VADD174 pKa = 5.48RR175 pKa = 11.84IGGGDD180 pKa = 3.23SADD183 pKa = 3.34NVNIGEE189 pKa = 4.2MAAYY193 pKa = 10.27NYY195 pKa = 9.48GALIGIDD202 pKa = 4.42PITTGTPTVYY212 pKa = 10.91DD213 pKa = 4.37LDD215 pKa = 4.04TQKK218 pKa = 11.16KK219 pKa = 10.12VDD221 pKa = 3.75IQDD224 pKa = 3.7EE225 pKa = 4.3DD226 pKa = 3.56WLDD229 pKa = 3.74NLLTSAA235 pKa = 4.96

Molecular weight: 24.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A0N327|A0A1A0N327_9MYCO ATP-sulfurylase small subunit (Fragment) OS=Mycobacteriaceae bacterium 1482268.1 OX=1834080 GN=A5662_21490 PE=3 SV=1
MM1 pKa = 7.18GRR3 pKa = 11.84VIAVVVGVVIALFGLLFALQGFGVVGGSPMSNTTTWSILGPIIALIGVAIAVGAARR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84PP63 pKa = 3.12

Molecular weight: 6.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4776

0

4776

1485318

31

4200

311.0

33.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.678 ± 0.05

0.819 ± 0.011

6.428 ± 0.031

5.416 ± 0.035

3.13 ± 0.019

8.682 ± 0.03

2.189 ± 0.017

4.429 ± 0.024

2.467 ± 0.026

9.666 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.125 ± 0.013

2.364 ± 0.022

5.752 ± 0.03

2.977 ± 0.018

7.128 ± 0.037

5.43 ± 0.023

5.947 ± 0.023

8.668 ± 0.032

1.515 ± 0.013

2.191 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski