Dehalogenimonas formicexedens
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2117 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1P8F4T9|A0A1P8F4T9_9CHLR tRNA nucleotidyltransferase (CCA-adding enzyme) OS=Dehalogenimonas formicexedens OX=1839801 GN=cca PE=3 SV=1
MM1 pKa = 7.48 TKK3 pKa = 10.24 KK4 pKa = 8.73 QTLFYY9 pKa = 10.22 KK10 pKa = 10.35 VAIIAAGLLATMAMLFAAIGPTKK33 pKa = 9.96 TDD35 pKa = 3.2 AAGGSVTQTSLGWRR49 pKa = 11.84 NGPNAQVWGNGNYY62 pKa = 10.45 GEE64 pKa = 4.41 YY65 pKa = 10.26 PEE67 pKa = 5.1 GDD69 pKa = 3.16 YY70 pKa = 10.11 WQSQILITNGTSAAIDD86 pKa = 3.08 ISGFGFILDD95 pKa = 4.73 FYY97 pKa = 11.21 NSGTVGVDD105 pKa = 2.99 WVKK108 pKa = 11.11 NLAWSMTPSAYY119 pKa = 9.72 FVAGDD124 pKa = 4.35 GPSDD128 pKa = 3.67 RR129 pKa = 11.84 YY130 pKa = 10.38 PYY132 pKa = 8.64 TGHH135 pKa = 6.76 VAGDD139 pKa = 4.34 DD140 pKa = 3.46 IGLSDD145 pKa = 5.03 WKK147 pKa = 9.91 TGWTPFTPKK156 pKa = 10.02 VINMPWDD163 pKa = 4.32 PGTDD167 pKa = 3.16 SYY169 pKa = 11.08 ITTEE173 pKa = 4.15 SQTAPAASHH182 pKa = 6.23 FWGGFDD188 pKa = 4.18 VNPPPTMLQPGEE200 pKa = 3.99 SLAIYY205 pKa = 8.84 FEE207 pKa = 4.14 PHH209 pKa = 6.22 LALTFIWSHH218 pKa = 5.11 GAEE221 pKa = 4.02 NLLPQIDD228 pKa = 4.0 GVYY231 pKa = 8.96 PGSGGTPTTFDD242 pKa = 3.95 RR243 pKa = 11.84 PWTAQYY249 pKa = 10.92 NGAGFYY255 pKa = 10.21 PGSSLHH261 pKa = 6.2 GSIVYY266 pKa = 10.4 SGLKK270 pKa = 9.05 TFQLPSGAVVNGVISGYY287 pKa = 10.44 KK288 pKa = 10.44 YY289 pKa = 10.39 NDD291 pKa = 3.45 KK292 pKa = 10.85 DD293 pKa = 3.63 VSGTFTAGDD302 pKa = 3.47 VGIPGWPIKK311 pKa = 10.57 LYY313 pKa = 9.62 ATVEE317 pKa = 4.27 GVPITVSTVTDD328 pKa = 3.42 SNGFYY333 pKa = 10.5 QFTNLPYY340 pKa = 8.7 GTYY343 pKa = 9.71 SVKK346 pKa = 10.21 EE347 pKa = 3.94 APEE350 pKa = 4.19 NGASIPAGYY359 pKa = 10.53 EE360 pKa = 3.85 NFVHH364 pKa = 6.7 SFPANGTISGIVLTSPAPVSANNNFFNYY392 pKa = 8.3 GTGKK396 pKa = 8.74 LTVTKK401 pKa = 10.26 SVVLGSVVNPAGISEE416 pKa = 4.6 TFTIHH421 pKa = 3.52 VTGPGGYY428 pKa = 9.17 SHH430 pKa = 8.08 DD431 pKa = 3.5 ITFTLTNGVLQSPTSVTLEE450 pKa = 3.74 GLIPGSYY457 pKa = 8.74 TVSEE461 pKa = 4.72 DD462 pKa = 3.43 NPGAEE467 pKa = 4.02 WTVTGGGSVTVNSGQTATSTVTNTFKK493 pKa = 10.76 PGSLEE498 pKa = 3.57 ITKK501 pKa = 10.69 VIDD504 pKa = 3.45 FNGAVNSASIDD515 pKa = 3.25 EE516 pKa = 4.48 TFTVKK521 pKa = 10.25 VVGPSYY527 pKa = 10.14 PGEE530 pKa = 4.35 GTTHH534 pKa = 6.54 QFVLDD539 pKa = 3.74 NGAIVAPNPWLLTGLIPGDD558 pKa = 3.66 YY559 pKa = 10.34 TITEE563 pKa = 3.98 VDD565 pKa = 4.91 AGAEE569 pKa = 3.94 WTEE572 pKa = 4.2 TVPASAVAVSAGGVATADD590 pKa = 3.39 VTNDD594 pKa = 3.48 YY595 pKa = 11.16 VPGSLEE601 pKa = 3.57 ITKK604 pKa = 10.69 VIDD607 pKa = 3.45 FNGAVNSASIDD618 pKa = 3.25 EE619 pKa = 4.48 TFTVKK624 pKa = 10.25 VVGPSYY630 pKa = 10.14 PGEE633 pKa = 4.35 GTTHH637 pKa = 6.54 QFVLDD642 pKa = 3.74 NGAIVAPNPWLLTGLIPGDD661 pKa = 3.66 YY662 pKa = 10.34 TITEE666 pKa = 3.98 VDD668 pKa = 4.91 AGAEE672 pKa = 3.94 WTEE675 pKa = 4.2 TVPASAVAVSAGVKK689 pKa = 8.85 ATADD693 pKa = 3.44 VTNDD697 pKa = 3.48 YY698 pKa = 11.16 VPGSLEE704 pKa = 3.42 ITKK707 pKa = 10.31 IIDD710 pKa = 3.12 FNGAVNSASIDD721 pKa = 3.25 EE722 pKa = 4.48 TFTVKK727 pKa = 10.25 VVGPSYY733 pKa = 10.14 PGEE736 pKa = 4.35 GTTHH740 pKa = 6.54 QFVLDD745 pKa = 3.74 NGAIVAPNPWLLTGLIPGDD764 pKa = 3.66 YY765 pKa = 10.34 TITEE769 pKa = 3.98 VDD771 pKa = 4.91 AGAEE775 pKa = 3.94 WTEE778 pKa = 4.2 TVPASAVAVSAGVKK792 pKa = 8.85 ATADD796 pKa = 3.44 VTNDD800 pKa = 3.48 YY801 pKa = 11.16 VPGSLEE807 pKa = 3.57 ITKK810 pKa = 10.69 VIDD813 pKa = 3.45 FNGAVNSASIDD824 pKa = 3.25 EE825 pKa = 4.48 TFTVKK830 pKa = 10.25 VVGPSYY836 pKa = 10.14 PGEE839 pKa = 4.35 GTTHH843 pKa = 6.54 QFVLDD848 pKa = 3.74 NGAIVAPNPWLLTGLIPGDD867 pKa = 3.66 YY868 pKa = 10.34 TITEE872 pKa = 3.98 VDD874 pKa = 4.91 AGAEE878 pKa = 3.94 WTEE881 pKa = 4.2 TVPASAVAVSAGGVATADD899 pKa = 3.39 VTNDD903 pKa = 3.48 YY904 pKa = 11.16 VPGSLEE910 pKa = 3.57 ITKK913 pKa = 10.69 VIDD916 pKa = 3.45 FNGAVNSASIDD927 pKa = 3.25 EE928 pKa = 4.48 TFTVKK933 pKa = 10.25 VVGPSYY939 pKa = 10.14 PGEE942 pKa = 4.35 GTTHH946 pKa = 6.54 QFVLDD951 pKa = 3.74 NGAIVAPNPWLLTGLIPGDD970 pKa = 3.66 YY971 pKa = 10.34 TITEE975 pKa = 3.98 VDD977 pKa = 4.91 AGAEE981 pKa = 3.94 WTEE984 pKa = 4.2 TVPASAVAVSAGVKK998 pKa = 8.85 ATADD1002 pKa = 3.44 VTNDD1006 pKa = 3.48 YY1007 pKa = 11.16 VPGSLEE1013 pKa = 3.57 ITKK1016 pKa = 10.69 VIDD1019 pKa = 3.45 FNGAVNSASIDD1030 pKa = 3.25 EE1031 pKa = 4.48 TFTVKK1036 pKa = 10.25 VVGPSYY1042 pKa = 10.14 PGEE1045 pKa = 4.35 GTTHH1049 pKa = 6.54 QFVLDD1054 pKa = 3.74 NGAIVAPNPWLLTGLIPGDD1073 pKa = 3.66 YY1074 pKa = 10.34 TITEE1078 pKa = 3.98 VDD1080 pKa = 4.91 AGAEE1084 pKa = 3.94 WTEE1087 pKa = 4.2 TVPASAVAVSAGVKK1101 pKa = 8.85 ATADD1105 pKa = 3.44 VTNDD1109 pKa = 3.48 YY1110 pKa = 11.16 VPGSLEE1116 pKa = 3.42 ITKK1119 pKa = 10.31 IIDD1122 pKa = 3.12 FNGAVNSASIDD1133 pKa = 3.25 EE1134 pKa = 4.48 TFTVKK1139 pKa = 10.25 VVGPSYY1145 pKa = 10.14 PGEE1148 pKa = 4.35 GTTHH1152 pKa = 6.54 QFVLDD1157 pKa = 3.74 NGAIVAPNPWLLTGLIPGDD1176 pKa = 3.66 YY1177 pKa = 10.34 TITEE1181 pKa = 3.98 VDD1183 pKa = 4.91 AGAEE1187 pKa = 3.94 WTEE1190 pKa = 4.2 TVPASAVAVSAGVKK1204 pKa = 8.85 ATADD1208 pKa = 3.44 VTNDD1212 pKa = 3.48 YY1213 pKa = 11.16 VPGSLEE1219 pKa = 3.57 ITKK1222 pKa = 10.69 VIDD1225 pKa = 3.45 FNGAVNSASIDD1236 pKa = 3.25 EE1237 pKa = 4.48 TFTVKK1242 pKa = 10.25 VVGPSYY1248 pKa = 10.14 PGEE1251 pKa = 4.35 GTTHH1255 pKa = 6.54 QFVLDD1260 pKa = 3.74 NGAIVAPNPWLLTGLIPGDD1279 pKa = 3.66 YY1280 pKa = 10.34 TITEE1284 pKa = 3.98 VDD1286 pKa = 4.91 AGAEE1290 pKa = 3.94 WTEE1293 pKa = 4.2 TVPASAVAVSAGGVATADD1311 pKa = 3.39 VTNDD1315 pKa = 3.48 YY1316 pKa = 11.16 VPGSLEE1322 pKa = 3.57 ITKK1325 pKa = 10.69 VIDD1328 pKa = 3.45 FNGAVNSASIDD1339 pKa = 3.25 EE1340 pKa = 4.48 TFTVKK1345 pKa = 10.25 VVGPSYY1351 pKa = 10.14 PGEE1354 pKa = 4.35 GTTHH1358 pKa = 6.54 QFVLDD1363 pKa = 3.74 NGAIVAPNPWLLTGLIPGDD1382 pKa = 3.66 YY1383 pKa = 10.34 TITEE1387 pKa = 3.98 VDD1389 pKa = 4.91 AGAEE1393 pKa = 3.94 WTEE1396 pKa = 4.2 TVPASAVAVSAGVKK1410 pKa = 8.85 ATADD1414 pKa = 3.44 VTNDD1418 pKa = 3.48 YY1419 pKa = 11.16 VPGSLEE1425 pKa = 3.42 ITKK1428 pKa = 10.31 IIDD1431 pKa = 3.12 FNGAVNSASIDD1442 pKa = 3.25 EE1443 pKa = 4.48 TFTVKK1448 pKa = 10.25 VVGPSYY1454 pKa = 10.14 PGEE1457 pKa = 4.35 GTTHH1461 pKa = 6.54 QFVLDD1466 pKa = 3.74 NGAIVAPNPWLLTGLIPGDD1485 pKa = 3.66 YY1486 pKa = 10.34 TITEE1490 pKa = 3.98 VDD1492 pKa = 4.91 AGAEE1496 pKa = 3.94 WTEE1499 pKa = 4.2 TVPASAVAVSAGGVATADD1517 pKa = 3.39 VTNDD1521 pKa = 3.48 YY1522 pKa = 11.16 VPGSLEE1528 pKa = 3.57 ITKK1531 pKa = 10.69 VIDD1534 pKa = 3.45 FNGAVNSASIDD1545 pKa = 3.25 EE1546 pKa = 4.48 TFTVKK1551 pKa = 10.25 VVGPSYY1557 pKa = 10.14 PGEE1560 pKa = 4.35 GTTHH1564 pKa = 6.54 QFVLDD1569 pKa = 3.74 NGAIVAPNPWLLTGLIPGDD1588 pKa = 3.66 YY1589 pKa = 10.34 TITEE1593 pKa = 3.98 VDD1595 pKa = 4.91 AGAEE1599 pKa = 3.94 WTEE1602 pKa = 4.2 TVPASAVAVSAGVKK1616 pKa = 8.85 ATADD1620 pKa = 3.44 VTNDD1624 pKa = 3.48 YY1625 pKa = 11.16 VPGSLEE1631 pKa = 3.42 ITKK1634 pKa = 10.31 IIDD1637 pKa = 3.12 FNGAVNSASIDD1648 pKa = 3.25 EE1649 pKa = 4.48 TFTVKK1654 pKa = 10.25 VVGPSYY1660 pKa = 10.14 PGEE1663 pKa = 4.35 GTTHH1667 pKa = 6.54 QFVLDD1672 pKa = 3.74 NGAIVAPNPWLLTGLIPGDD1691 pKa = 3.66 YY1692 pKa = 10.34 TITEE1696 pKa = 3.98 VDD1698 pKa = 4.91 AGAEE1702 pKa = 3.94 WTEE1705 pKa = 4.2 TVPASAVAVSAGVKK1719 pKa = 8.85 ATADD1723 pKa = 3.47 VTNDD1727 pKa = 3.51 FVPGSLTITKK1737 pKa = 9.72 VVVLGAYY1744 pKa = 8.79 PFASTTTLDD1753 pKa = 3.44 FTVKK1757 pKa = 8.92 VTGPSYY1763 pKa = 10.21 PGPDD1767 pKa = 3.08 GTTLTFNLVNGVISGAQTLDD1787 pKa = 3.21 NLIPGVYY1794 pKa = 8.82 TVTEE1798 pKa = 4.15 TDD1800 pKa = 3.74 PGVAWTVTNLTGNVTVDD1817 pKa = 3.61 PGTPTATRR1825 pKa = 11.84 TITNTLKK1832 pKa = 10.63 QPNTTVTIGSDD1843 pKa = 2.83 TWEE1846 pKa = 4.28 TFPGGNVTITVTEE1859 pKa = 4.38 EE1860 pKa = 3.72 NTGDD1864 pKa = 3.58 VPLSNVSVVVTQNDD1878 pKa = 4.2 GSPLTLSAPPTSGDD1892 pKa = 3.52 ANTDD1896 pKa = 3.8 GILDD1900 pKa = 3.94 TDD1902 pKa = 4.25 EE1903 pKa = 3.95 TWTWVYY1909 pKa = 9.69 LTTISVDD1916 pKa = 3.35 TTFQATGHH1924 pKa = 5.69 GTDD1927 pKa = 4.09 PLGNDD1932 pKa = 2.75 ITVPNYY1938 pKa = 7.96 PTEE1941 pKa = 4.0 QDD1943 pKa = 3.51 DD1944 pKa = 4.48 VFVEE1948 pKa = 4.45 VNGATRR1954 pKa = 11.84 TIGFWKK1960 pKa = 7.6 THH1962 pKa = 4.69 WDD1964 pKa = 3.48 FTEE1967 pKa = 4.71 HH1968 pKa = 6.5 VFTDD1972 pKa = 3.74 PTGLNSNINLGTWGGKK1988 pKa = 6.03 TWTITTMEE1996 pKa = 4.17 QLMGLLWANTAKK2008 pKa = 10.78 NADD2011 pKa = 3.86 EE2012 pKa = 4.39 TSSSRR2017 pKa = 11.84 LTIDD2021 pKa = 2.86 QAKK2024 pKa = 9.6 IHH2026 pKa = 5.92 TAQQALAAILNDD2038 pKa = 3.38 AMAGGAPLPVTLTDD2052 pKa = 3.06 IVGVLEE2058 pKa = 4.57 GNSIGQVRR2066 pKa = 11.84 SLGSTLDD2073 pKa = 3.41 AYY2075 pKa = 11.42 NNSGDD2080 pKa = 4.58 NIALDD2085 pKa = 3.88 PSLPPTKK2092 pKa = 10.3 KK2093 pKa = 10.48 GDD2095 pKa = 3.39 EE2096 pKa = 4.37 GKK2098 pKa = 9.83 PKK2100 pKa = 10.63 DD2101 pKa = 4.07 LADD2104 pKa = 3.93 IPWANTTPEE2113 pKa = 3.88 APKK2116 pKa = 10.63 GKK2118 pKa = 10.09 KK2119 pKa = 9.15
Molecular weight: 219.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.843
IPC2_protein 3.859
IPC_protein 3.897
Toseland 3.681
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.783
Rodwell 3.719
Grimsley 3.579
Solomon 3.872
Lehninger 3.834
Nozaki 3.973
DTASelect 4.215
Thurlkill 3.706
EMBOSS 3.795
Sillero 4.012
Patrickios 2.473
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.914
Protein with the highest isoelectric point:
>tr|A0A1P8F9V0|A0A1P8F9V0_9CHLR 4Fe-4S binding domain-containing protein OS=Dehalogenimonas formicexedens OX=1839801 GN=Dform_01895 PE=4 SV=1
MM1 pKa = 7.87 PDD3 pKa = 2.77 IMSRR7 pKa = 11.84 AIIQSGGGGGYY18 pKa = 9.41 IEE20 pKa = 4.81 GGRR23 pKa = 11.84 VKK25 pKa = 10.56 NPPLQCSDD33 pKa = 3.64 SQTVAAGLQTSADD46 pKa = 3.33 PSLRR50 pKa = 11.84 QNNEE54 pKa = 3.35 RR55 pKa = 11.84 CVRR58 pKa = 11.84 GWNGGGRR65 pKa = 11.84 VKK67 pKa = 10.65 NPPLQCPGAKK77 pKa = 9.51 PGQPVSRR84 pKa = 11.84 PAQIPVFAKK93 pKa = 8.57 ITRR96 pKa = 11.84 GAFDD100 pKa = 4.89 DD101 pKa = 4.28 EE102 pKa = 4.79 PGEE105 pKa = 4.69 GGLKK109 pKa = 8.37 TRR111 pKa = 11.84 PYY113 pKa = 10.03 DD114 pKa = 3.33 TGRR117 pKa = 11.84 KK118 pKa = 8.31 PGAPGVLWRR127 pKa = 11.84 SRR129 pKa = 11.84 RR130 pKa = 11.84 RR131 pKa = 11.84 RR132 pKa = 11.84 DD133 pKa = 2.96 SGLRR137 pKa = 11.84 RR138 pKa = 11.84 NDD140 pKa = 2.9 KK141 pKa = 10.69 RR142 pKa = 11.84 HH143 pKa = 6.25 LKK145 pKa = 10.76 DD146 pKa = 3.04 EE147 pKa = 4.29 TGRR150 pKa = 11.84 AGG152 pKa = 3.39
Molecular weight: 16.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 9.589
IPC_protein 10.467
Toseland 10.877
ProMoST 10.657
Dawson 10.921
Bjellqvist 10.672
Wikipedia 11.169
Rodwell 11.067
Grimsley 10.95
Solomon 11.125
Lehninger 11.082
Nozaki 10.862
DTASelect 10.657
Thurlkill 10.862
EMBOSS 11.286
Sillero 10.862
Patrickios 10.804
IPC_peptide 11.125
IPC2_peptide 9.838
IPC2.peptide.svr19 8.768
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2117
0
2117
607201
29
2119
286.8
31.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.503 ± 0.06
1.161 ± 0.023
5.135 ± 0.034
6.152 ± 0.062
4.038 ± 0.04
8.002 ± 0.056
1.862 ± 0.021
6.676 ± 0.047
5.169 ± 0.052
9.881 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.444 ± 0.028
3.458 ± 0.031
4.792 ± 0.038
3.059 ± 0.035
5.67 ± 0.057
6.145 ± 0.039
5.581 ± 0.061
7.328 ± 0.048
1.168 ± 0.021
2.777 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here